Coupling Microfluidic Platforms, Microfabrication, and Tissue Engineered Scaffolds to Investigate Tumor Cells Mechanobiology

Millet, Martial; Messaoud, Raoua Ben; Luthold, Carole; Bordeleau, François

doi:10.3390/mi10060418

Cited by 16 publications

(12 citation statements)

References 145 publications

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“…The authors claimed that a high degree of similarity, including tissues ECM phenotypic activation, metalloproteinases (MMPs) overexpression, and endogenous collagen network architecture time evolution, was observed utilizing this microfluidic assay when compared with established in vivo models. The role of non-malignant cells in breast TME is also critical in metastasis, notably in cell migration ( Millet et al, 2019 ). Truong et al (2019) established a microfluidics-based 3D-organotypic model to characterize breast tumor invasion that is driven by stroma activation.…”

Section: Invasionmentioning

confidence: 99%

Biomimetic Microfluidic Platforms for the Assessment of Breast Cancer Metastasis

Sigdel

Gupta

Faizee

et al. 2021

Front. Bioeng. Biotechnol.

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Of around half a million women dying of breast cancer each year, more than 90% die due to metastasis. Models necessary to understand the metastatic process, particularly breast cancer cell extravasation and colonization, are currently limited and urgently needed to develop therapeutic interventions necessary to prevent breast cancer metastasis. Microfluidic approaches aim to reconstitute functional units of organs that cannot be modeled easily in traditional cell culture or animal studies by reproducing vascular networks and parenchyma on a chip in a three-dimensional, physiologically relevant in vitro system. In recent years, microfluidics models utilizing innovative biomaterials and micro-engineering technologies have shown great potential in our effort of mechanistic understanding of the breast cancer metastasis cascade by providing 3D constructs that can mimic in vivo cellular microenvironment and the ability to visualize and monitor cellular interactions in real-time. In this review, we will provide readers with a detailed discussion on the application of the most up-to-date, state-of-the-art microfluidics-based breast cancer models, with a special focus on their application in the engineering approaches to recapitulate the metastasis process, including invasion, intravasation, extravasation, breast cancer metastasis organotropism, and metastasis niche formation.

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Section: Invasionmentioning

confidence: 99%

Biomimetic Microfluidic Platforms for the Assessment of Breast Cancer Metastasis

Sigdel

Gupta

Faizee

et al. 2021

Front. Bioeng. Biotechnol.

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“…In order to get closer to the tissue environment, natural biomaterials are used in microfabrication, though they are costly and control of their physical properties cannot be performed. For instance, collagen-based scaffolds give the possibility of more physiological culture systems since it is an intrinsic extracellular matrix (ECM) component and the 3D architecture of the collagen meshwork is close to the in vivo context (Millet et al, 2019). However, more complex ECM-based hydrogels can be used.…”

Section: Mimetism Of the Intestinal Tissue Biochemistrymentioning

confidence: 99%

Human Microphysiological Models of Intestinal Tissue and Gut Microbiome

Steinway

Saleh

Koo

et al. 2020

Front. Bioeng. Biotechnol.

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The gastrointestinal (GI) tract is a complex system responsible for nutrient absorption, digestion, secretion, and elimination of waste products that also hosts immune surveillance, the intestinal microbiome, and interfaces with the nervous system. Traditional in vitro systems cannot harness the architectural and functional complexity of the GI tract. Recent advances in organoid engineering, microfluidic organs-on-a-chip technology, and microfabrication allows us to create better in vitro models of human organs/tissues. These micro-physiological systems could integrate the numerous cell types involved in GI development and physiology, including intestinal epithelium, endothelium (vascular), nerve cells, immune cells, and their interplay/cooperativity with the microbiome. In this review, we report recent progress in developing microphysiological models of the GI systems. We also discuss how these models could be used to study normal intestinal physiology such as nutrient absorption, digestion, and secretion as well as GI infection, inflammation, cancer, and metabolism.

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“…It is known that the nuclei of cancer cells are softer than those of healthy cells. On micropatterned surfaces, these nuclei show different levels of morphological distortions as a result of force transduction, which helps migration and metastasis [40][41][42][43][44]. Therefore, the deformability of cancer cells, especially their nuclei, is being studied in the discrimination of healthy and diseased cells.…”

Section: Introductionmentioning

confidence: 99%

“…Biomaterials can be used to microfabricate surfaces. Both surface chemical and physical properties can be controlled, and microfabrication allows the precise control of surface topography [41]. Using such microfabricated substrates made from biomaterials enables us to study cancer cells and their responses to their physical microenvironment [42].…”

Section: Introductionmentioning

confidence: 99%

A Cell Culture Chip with Transparent, Micropillar-Decorated Bottom for Live Cell Imaging and Screening of Breast Cancer Cells

et al. 2022

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In the recent years, microfabrication technologies have been widely used in cell biology, tissue engineering, and regenerative medicine studies. Today, the implementation of microfabricated devices in cancer research is frequent and advantageous because it enables the study of cancer cells in controlled microenvironments provided by the microchips. Breast cancer is one of the most common cancers in women, and the way breast cancer cells interact with their physical microenvironment is still under investigation. In this study, we developed a transparent cell culture chip (Ch-Pattern) with a micropillar-decorated bottom that makes live imaging and monitoring of the metabolic, proliferative, apoptotic, and morphological behavior of breast cancer cells possible. The reason for the use of micropatterned surfaces is because cancer cells deform and lose their shape and acto-myosin integrity on micropatterned substrates, and this allows the quantification of the changes in morphology and through that identification of the cancerous cells. In the last decade, cancer cells were studied on micropatterned substrates of varying sizes and with a variety of biomaterials. These studies were conducted using conventional cell culture plates carrying patterned films. In the present study, cell culture protocols were conducted in the clear-bottom micropatterned chip. This approach adds significantly to the current knowledge and applications by enabling low-volume and high-throughput processing of the cell behavior, especially the cell–micropattern interactions. In this study, two different breast cancer cell lines, MDA-MB-231 and MCF-7, were used. MDA-MB-231 cells are invasive and metastatic, while MCF-7 cells are not metastatic. The nuclei of these two cell types deformed to distinctly different levels on the micropatterns, had different metabolic and proliferation rates, and their cell cycles were affected. The Ch-Pattern chips developed in this study proved to have significant advantages when used in the biological analysis of live cells and highly beneficial in the study of screening breast cancer cell–substrate interactions in vitro.

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Coupling Microfluidic Platforms, Microfabrication, and Tissue Engineered Scaffolds to Investigate Tumor Cells Mechanobiology

Cited by 16 publications

References 145 publications

Biomimetic Microfluidic Platforms for the Assessment of Breast Cancer Metastasis

Biomimetic Microfluidic Platforms for the Assessment of Breast Cancer Metastasis

Human Microphysiological Models of Intestinal Tissue and Gut Microbiome

A Cell Culture Chip with Transparent, Micropillar-Decorated Bottom for Live Cell Imaging and Screening of Breast Cancer Cells

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