Coupling live-cell imaging and in situ isolation of the same single cell to profile the transient states of predicted drug-tolerant cells

Bian, Benjamin; Paquet, Agnès; Arguel, Marie-Jeanne; Meyer, Mickael; Peyre, Ludovic; Chalabi, Asma; Péré, Marielle; Lebrigand, Kévin; Waldmann, Rainer; Barbry, Pascal; Hofman, Paul; Roux, Jérémie

doi:10.1016/j.xpro.2022.101600

Cited by 1 publication

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References 8 publications

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“…Arc-well, a high-throughput single-cell DNA sequencing method compatible with formalin-fixed and paraffin-embedding materials, is a good example [102]. Fate-seq, an approach to isolate single cells in their transient states of drug sensitivity or tolerance before profiling, has also been proposed [103]. The authors believe that, in the future, the application of a single-cell sequencing technique will help researchers to find and solve the heterogeneity of the diapause-like DTP state so as to better treat tumor drug resistance.…”

Section: Perspectives and Conclusionmentioning

confidence: 99%

Diapause-like Drug-Tolerant Persister State: The Key to Nirvana Rebirth

Chen,

Jin

2024

Medicina

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Cancer is one of the leading causes of death in the world. Various drugs have been developed to eliminate it but to no avail because a tumor can go into dormancy to avoid therapy. In the past few decades, tumor dormancy has become a popular topic in cancer therapy. Recently, there has been an important breakthrough in the study of tumor dormancy. That is, cancer cells can enter a reversible drug-tolerant persister (DTP) state to avoid therapy, but no exact mechanism has been found. The study of the link between the DTP state and diapause seems to provide an opportunity for a correct understanding of the mechanism of the DTP state. Completely treating cancer and avoiding dormancy by targeting the expression of key genes in diapause are possible. This review delves into the characteristics of the DTP state and its connection with embryonic diapause, and possible treatment strategies are summarized. The authors believe that this review will promote the development of cancer therapy.

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Section: Perspectives and Conclusionmentioning

confidence: 99%