Coupling between DNA replication, segregation, and the onset of constriction in Escherichia coli

Krishnan, Sriram Tiruvadi; Männik, Jaana; Kar, Prathitha; Lin, Jie; Amir, Ariel; Männik, Jaan

doi:10.1016/j.celrep.2022.110539

Cited by 18 publications

(42 citation statements)

References 73 publications

(119 reference statements)

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“…Similarly, nucleoid separation could also occur in a previous generation. This was showcased in DAPI demographs of the parent strain grown in the richest nutrient conditions, where cells were already born with two separated nucleoids (Figure 3C), as described before 53 .…”

Section: Resultssupporting

confidence: 67%

“…Throughout our analysis, we did not find any direct connection between DNA replication and division events across strains and media, supporting the idea that the DNA replication and cell division cycles operate largely independently. This is important as a wealth of models have been proposed to explain if and how cell division is controlled by DNA replication 13,21,39,44,53,66,67,77–80 . DNA replication and cell constriction are, however, indirectly connected by virtue of their dependence on (different aspects of) cell size, which may help explain the appearance of conflicting results across previous studies.…”

Section: Discussionmentioning

confidence: 99%

Section: Points Of Control and Integrationmentioning

confidence: 99%

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Apparent simplicity and emergent robustness in bacterial cell cycle control

Govers

Campos

Tyagi

et al. 2023

Preprint

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To examine how bacteria achieve robust cell proliferation across diverse conditions, we developed a method that quantifies 77 cell morphological, cell cycle and growth phenotypes of a fluorescently-labeled Escherichia coli strain and >800 gene deletion derivatives under multiple nutrient conditions. This approach revealed extensive phenotypic plasticity and deviating mutant phenotypes were often found to be nutrient-dependent. From this broad phenotypic landscape emerged simple and robust unifying rules (laws) that connect DNA replication initiation, nucleoid segregation, FtsZ-ring formation, and cell constriction to specific aspects of cell size (volume, length, or added length). Furthermore, completion of cell division followed the initiation of cell constriction after a constant time delay across strains and nutrient conditions, identifying cell constriction as a key control point for cell size determination. Our work provides a systems-level understanding of the design principles by which E. coli integrates cell cycle processes and growth rate with cell size to achieve its robust proliferative capability.

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Section: Resultssupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Points Of Control and Integrationmentioning

confidence: 99%

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Apparent simplicity and emergent robustness in bacterial cell cycle control

Govers

Campos

Tyagi

et al. 2023

Preprint

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“…The initial increase in the amount of FtsN at midcell was accompanied by an increase in the midcell phase signal ( Fig. 1A and B , bottom), indicating the onset of constriction approximately coincided with FtsN midcell recruitment as concluded before ( 16 , 18 , 20 , 40 ).…”

Section: Resultsmentioning

confidence: 64%

“…To investigate the recruitment kinetics of FtsN to the divisome in live cells, we used quantitative fluorescence microscopy of a functional N-terminal fusion of Ypet to FtsN (Ypet-FtsN) ( 40 ). We studied the kinetics of FtsN relative to the formation of the Z ring.…”

Section: Resultsmentioning

confidence: 99%

Cell Cycle-Dependent Recruitment of FtsN to the Divisome in Escherichia coli

Männik

Pichoff

Lutkenhaus

2022

mBio

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Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

Wang,

Chen,

Jin

et al. 2023

Advanced Science

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Prokaryotic genomes are generally organized in haploid. In synthetic biological research, efficient chassis cells must be constructed to produce bio‐based products. Here, the essential division of the ftsZ gene to create functional polyploid E. coli is regulated. The artificial polyploid E. coli containing 2–4 chromosomes is confirmed through PCR amplification, terminator localization, and flow cytometry. The polyploid E. coli exhibits a larger cell size, and its low pH tolerance and acetate resistance are stronger than those of haploid E. coli. Transcriptome analysis shows that the genes of the cell's main functional pathways are significantly upregulated in the polyploid E. coli. These advantages of the polyploid E. coli results in the highest reported L‐threonine yield (160.3 g L−1) in fed‐batch fermentation to date. In summary, an easy and convenient method for constructing polyploid E. coli and demonstrated its application in L‐threonine production is developed. This work provides a new approach for creating an excellent host strain for biochemical production and studying the evolution of prokaryotes and their chromosome functions.

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Coupling between DNA replication, segregation, and the onset of constriction in Escherichia coli

Cited by 18 publications

References 73 publications

Apparent simplicity and emergent robustness in bacterial cell cycle control

Apparent simplicity and emergent robustness in bacterial cell cycle control

Cell Cycle-Dependent Recruitment of FtsN to the Divisome in Escherichia coli

Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

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