Coupled sodium and chloride transport into plasma membrane vesicles prepared from dogfish rectal gland

Eveloff, Jill; Kinne, Rolf; Kinne-Saffran, Evamaria; Murer, Heini; Silva, Patricio; Epstein, Franklin H.; Stoff, Jeffrey S.; Kinter, William B.

doi:10.1007/bf00584439

Cited by 88 publications

(34 citation statements)

References 13 publications

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“…The isolated perfused elasmobranch rectal gland is a tubular epithelial structure that has been widely used as a model for sodium coupled secondary active chloride transport (24,25). Previous studies have indicated striking similarities between chloride transport in this tissue and the mammalian thick ascending limb of Henle's loop (17,24,26). The present studies demonstrate the presence ofa high affinity inhibitory Al adenosine receptor in the rectal gland that potently inhibits hormone-stimulated chloride secretion.…”

Section: Introductionsupporting

confidence: 59%

“…Previous studies have underscored the similarities between chloride secretion in tubules of the rectal gland and chloride reabsorption in the mammalian medullary thick ascending limb of Henle's loop (mTAL) (17,24,26). Our laboratory recently has demonstrated a high density of A1 receptor binding sites in rat-mTAL using high resolution in vitro autoradiography (48).…”

Section: Cptmentioning

confidence: 99%

See 1 more Smart Citation

A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP.

Kelley

Poeschla²,

Barron³

et al. 1990

J. Clin. Invest.

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In the in vitro perfused rectal gland of the dogfish shark (Squalus acanthias), the adenosine analogue 2-chloroadenosine (2Clado) completely and reversibly inhibited forskolinstimulated chloride secretion with an IC50 of 5 nM. Other Al receptor agonists including cyclohexyladenosine (CHA), N-ethylcarboxamideadenosine (NECA) and R-phenylisopropyladenosine (R-PIA) also completely inhibited forskolin stimulated chloride secretion. The "S" stereoisomer of PIA (S-PIA) was a less potent inhibitor of forskolin stimulated chloride secretion, consistent with the affinity profile of PIA stereoisomers for an Al receptor. The adenosine receptor antagonists 8-phenyltheophylline and 8-cyclopentyltheophylline completely blocked the effect of 2Clado to inhibit forskolin-stimulated chloride secretion. When chloride secretion and tissue cyclic (c)AMP content were determined simultaneously in perfused glands, 2Clado completely inhibited secretion but only inhibited forskolin stimulated cAMP accumulation by 3440%, indicating that the mechanism of inhibition of secretion by 2Clado is at least partially cAMP independent. Consistent with these results, Al receptor agonists only modestly inhibited (9-15%) forskolin stimulated adenylate cyclase activity and 2Clado markedly inhibited chloride secretion stimulated by a permeant cAMP analogue, 8-chlorophenylthio cAMP (8CPT cAMP). These findings provide the first evidence for a high affinity Al adenosine receptor that inhibits hormone stimulated ion transport in a model epithelia. A major portion of this inhibition occurs by a mechanism that is independent of the cAMP messenger system. (J. Clin. Invest.

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Section: Introductionsupporting

confidence: 59%

Section: Cptmentioning

confidence: 99%

A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP.

Kelley

Poeschla²,

Barron³

et al. 1990

J. Clin. Invest.

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“…Thus, 14). Furosemide is also known to inhibit carriermediated cation-anion cotransport processes, such as Na+-Cl-cotransport in plasma membranes of the dogfish rectal gland (15). None of these agents has been reported to inhibit nonionic diffusion in any experimental system.…”

Section: Resultsmentioning

confidence: 99%

pH gradient-stimulated transport of urate and p-aminohippurate in dog renal microvillus membrane vesicles.

Blomstedt¹,

Aronson²

1980

J. Clin. Invest.

112

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“…This provides indirect evidence that coupled transport of Na+ and Cl-is important in lacrimal gland fluid secretion. That furosemide is an inhibitor of coupled transport of Na+ and Cl-has been demonstrated in basolateral plasma membrane vesicles prepared from the dogfish rectal gland (Eveloff et al 1978) and the brush border of flounder intestine (Frizzell et al 1979b). …”

Section: Discussionmentioning

confidence: 99%

“…A model which incorporates such an indirect role has been suggested for the dog-fish rectal gland (Silva, Stoff, Field, Fine, Forrest & Epstein, 1977) and is based on following rationale: baso-laterally located (Nat + K+)-activated ATPase directly provides the energy for extruding Na+ from the cell against its electrochemical gradient and, thereby, indirectly energizes transcellular Cl-movement mediated by a NaCl cotransport system located at the basolateral plasma membrane. To determine whether a similar model could account for lacrimal gland electrolyte and water secretion, the effects of ouabain and furosemide (an inhibitor of coupled transport of Na+ and Cl-(Eveloff, Kinne, Kinne-Saffran, Silva, Epstein, Stoff & Kinter, 1978;Frizzell, Smith, Vosburgh & Field, 1979b)) on ACh-induced secretion were studied. In addition, the (Na++K+)-activated ATPase was localized by the autoradiographic technique developed by Stirling (1972).…”

Section: Introductionmentioning

confidence: 99%

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

Dartt¹,

Møller²,

Poulsen³

1981

The Journal of Physiology

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SUMMARY1. The rate of acetylcholine (ACh)-induced fluid secretion was measured from the main excretory duct of rabbit lacrimal glands perfused in vivo with Krebs Henseleit bicarbonate solutions.2. Perfusion with ouabain (10-5 M) decreased the rate of lacrimal gland fluid secretion to 23 % of the control value.3. Perfusion with furosemide (10-4 and 1O-3 M), which has been shown to inhibit the coupled transport of Na+ and Cl-, reversibly decreased the rate of secretion to 43 and 33 % of the control value respectively. 6. It is concluded that ACh-induced secretion of electrolytes and water is dependent upon (Na+ + K+)-activated ATPase. In addition, coupled transport of Na+ and Cl-appears to be involved in secretion.7. Basolateral location of the (Na+ + K+)-activated ATPase implies that it plays an indirect role in electrolyte and water secretion. A possible role may be to energize a secondary active transport of Cl-that is mediated by a NaCl cotransport system.

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Coupled sodium and chloride transport into plasma membrane vesicles prepared from dogfish rectal gland

Cited by 88 publications

References 13 publications

A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP.

A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP.

pH gradient-stimulated transport of urate and p-aminohippurate in dog renal microvillus membrane vesicles.

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

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