The emergence of insecticides resistance by malaria vectors poses a threat to the deployment of bed nets for malaria control in Nigeria and Gombe State specifically. This study aimed at evaluating the resistance status of insecticides recommended by World Health Organization Pesticide Evaluation Scheme for control of major malaria vector Anopheles gambiae sl. Immature stages of Anopheles were collected from ten (10) communities between January -December, 2022 and reared to adulthood. Twenty-five (25) batches of glucose fed female 2-5 days old Anopheles were exposed to each of the four (4) WHO diagnostic tubes (replicate) containing deltametthrin (0.05%), DDT (4%), bendiocarb (0.1%), pirimiohos-methyl (0.25%) impregnated paper for onehour knockdown mortality and final mortality was recorded 24 hours' post-exposure. The enzyme that involve in breaking the resistance gene was also investigated using piperonyl butoxide (PBO). Death and alive mosquitoes were identified using morphological keys. All the test was conducted at the temperature 25-33˚C and relative humidity 60-80%. Resistance and susceptible mosquitoes was classified following World Health Organization criteria; mortality of 98-100 % indicating susceptible, 90-97 possible resistance that required confirmation and <90 indicating resistance. Anopheles were susceptible to pirimiphos-methyl (0.25%) and suspected resistant to bendiocarb (0.1%) (93-96%) but highly resistant with mortality (<90%) to DDT (4%) (3-74%) and deltamethrin (0.05%) (2 -88%) in six of the study communities. Pre-exposure of Anopheles to PBO allow deltamethrin (0.05%) to restore its' susceptibility (≥98). The increased resistance to deltamethrin in Anopheles could endanger the efficacy of LLINs used for malaria vector control.