Abstract:A non-refined, organic pumpkin seed oil (PSO) was chosen for the
isolation and structure verification of the rare vitamin E compound
γ-tocomonoenol (γ-T1). Initial measurements indicated the presence of
~0.4 mg γ-T1 per 100 g pumpkin seed oil. Saponification
of ~2 L pumpkin seed oil, followed by repeated
countercurrent chromatography (CCC) with the solvent system
n-hexane/benzotrifluoride/acetonitrile (10:3.5:6.5,
v/v/v) and silica gel column chromatography enabled
the isolation of 6.8 mg γ-T1 with a purity of… Show more
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