Counterbalancing anti-adhesive effects of Tenascin-C through fibronectin expression in endothelial cells

Radwańska, Agata; Grall, Dominique; Schaub, Sébastien; Divonne, Stéphanie Beghelli-de la Forest; Ciais, Delphine; Rekima, Samah; Rupp, Tristan; Sudaka, Anne; Orend, Gertraud; Obberghen-Schilling, Ellen Van

doi:10.1038/s41598-017-13008-9

Cited by 22 publications

(23 citation statements)

References 44 publications

(46 reference statements)

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“…This aspect is often overlooked in vitro and has direct repercussions in vivo since the ECM contains both pro-and anti-adhesive proteins. 75 Importantly, this is the first time that contact guidance induced by cadherins was observed. It was also shown that, in the case of spatially segregated adhesive molecules known to operate in distinct pathways, not only the balance but also the contrast in adhesive forces can generate contact guidance.…”

Section: Discussionmentioning

confidence: 99%

Cell–Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation

Saux

Wu²,

Toledo

et al. 2020

ACS Appl. Mater. Interfaces

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Contact guidance has been extensively explored using patterned adhesion functionalities that predominantly mimic cell–matrix interactions. Whether contact guidance can also be driven by other types of interactions, such as cell−cell adhesion, still remains a question. Herein, this query is addressed by engineering a set of microstrip patterns of (i) cell–cell adhesion ligands and (ii) segregated cell–cell and cell–matrix ligands as a simple yet versatile set of platforms for the guidance of spreading, adhesion, and differentiation of mesenchymal stem cells. It was unprecedently found that micropatterns of cell–cell adhesion ligands can induce contact guidance. Surprisingly, it was found that patterns of alternating cell–matrix and cell–cell strips also induce contact guidance despite providing a spatial continuum for cell adhesion. This guidance is believed to be due to the difference between the potencies of the two adhesions. Furthermore, patterns that combine the two segregated adhesion functionalities were shown to induce more human mesenchymal stem cell osteogenic differentiation than monofunctional patterns. This work provides new insight into the functional crosstalk between cell–cell and cell–matrix adhesions and, overall, further highlights the ubiquitous impact of the biochemical anisotropy of the extracellular environment on cell function.

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Section: Discussionmentioning

confidence: 99%

Cell–Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation

Saux

Wu²,

Toledo

et al. 2020

ACS Appl. Mater. Interfaces

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“…Indeed, Tenascin-C can exhibit repulsive properties, which may lead to a reduced aggregate formation. In this regard, a counterbalancing anti-adhesive effect of Tenascin-C through Fibronectin expression was reported in endothelial cells [127].…”

Section: Ecm Influence On Cluster Formation Of Rb Cellsmentioning

confidence: 97%

Expression Changes and Impact of the Extracellular Matrix on Etoposide Resistant Human Retinoblastoma Cell Lines

Reinhard

Wagner

Krämer

et al. 2020

IJMS

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Retinoblastoma (RB) represents the most common malignant childhood eye tumor worldwide. Several studies indicate that the extracellular matrix (ECM) plays a crucial role in tumor growth and metastasis. Moreover, recent studies indicate that the ECM composition might influence the development of resistance to chemotherapy drugs. The objective of this study was to evaluate possible expression differences in the ECM compartment of the parental human cell lines WERI-RB1 (retinoblastoma 1) and Y79 and their Etoposide resistant subclones via polymerase chain reaction (PCR). Western blot analyses were performed to analyze protein levels. To explore the influence of ECM molecules on RB cell proliferation, death, and cluster formation, WERI-RB1 and resistant WERI-ETOR cells were cultivated on Fibronectin, Laminin, Tenascin-C, and Collagen IV and analyzed via time-lapse video microscopy as well as immunocytochemistry. We revealed a significantly reduced mRNA expression of the proteoglycans Brevican, Neurocan, and Versican in resistant WERI-ETOR compared to sensitive WERI-RB1 cells. Also, for the glycoproteins α1-Laminin, Fibronectin, Tenascin-C, and Tenascin-R as well as Collagen IV, reduced expression levels were observed in WERI-ETOR. Furthermore, a downregulation was detected for the matrix metalloproteinases MMP2, MMP7, MMP9, the tissue-inhibitor of metalloproteinase TIMP2, the Integrin receptor subunits ITGA4, ITGA5 and ITGB1, and all receptor protein tyrosine phosphatase β/ζ isoforms. Downregulation of Brevican, Collagen IV, Tenascin-R, MMP2, TIMP2, and ITGA5 was also verified in Etoposide resistant Y79 cells compared to sensitive ones. Protein levels of Tenascin-C and MMP-2 were comparable in both WERI cell lines. Interestingly, Fibronectin displayed an apoptosis-inducing effect on WERI-RB1 cells, whereas an anti-apoptotic influence was observed for Tenascin-C. Conversely, proliferation of WERI-ETOR cells was enhanced on Tenascin-C, while an anti-proliferative effect was observed on Fibronectin. In WERI-ETOR, cluster formation was decreased on the substrates Collagen IV, Fibronectin, and Tenascin-C. Collectively, we noted a different ECM mRNA expression and behavior of Etoposide resistant compared to sensitive RB cells. These findings may indicate a key role of ECM components in chemotherapy resistance formation of RB.

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“…Remarkable is the expression of TNC in murine aortic ECs (Natarelli et al, 2018). The fact that TNC is commonly known as ECM component and TNC is not expressed in ECs (Radwanska et al, 2017) indicates a possible contamination in the reported study. Furthermore, expression of TFPI2 found in the human umbilical cord ECs (Voellenkle et al, 2016) is in agreement with previous findings (Beeravolu et al, 2016), whereas expression was not detected in the adult CaPECs.…”

Section: Discussionmentioning

confidence: 88%

Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels

et al. 2019

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Vasculature performs a critical function in tissue homeostasis, supply of oxygen and nutrients, and the removal of metabolic waste products. Vascular problems are implicated in a large variety of pathologies and accurate in vitro models resembling native vasculature are of great importance. Unfortunately, existing in vitro models do not sufficiently reflect their in vivo counterpart. The complexity of vasculature requires the examination of multiple cell types including endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), as well as vessel location in the body from which they originate. The use of canine blood vessels provides a way to study vasculature with similar vessel size and physiology compared to human vasculature. We report an isolation procedure that provides the possibility to isolate both the endothelial and smooth muscle cells from the same vessels simultaneously, enabling new opportunities in investigating vasculature behavior. Canine primary ECs and VSMCs were isolated from the vena cava, vena porta and aorta. All tissue sources were derived from three donors for accurate comparison and to reduce inter-animal variation. The isolation and purification of the two distinct cell types was confirmed by morphology, gene- and protein-expression and function. As both cell types can be derived from the same vessel, this approach allows accurate modeling of vascular diseases and can also be used more widely, for example, in vascular bioreactors and tissue engineering designs. Additionally, we identified several new genes that were highly expressed in canine ECs, which may become candidate genes for novel EC markers. In addition, we observed transcriptional and functional differences between arterial- and venous-derived endothelium. Further exploration of the transcriptome and physiology of arteriovenous differentiation of primary cells may have important implications for a better understanding of the fundamental behavior of the vasculature and pathogenesis of vascular disease.

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Counterbalancing anti-adhesive effects of Tenascin-C through fibronectin expression in endothelial cells

Cited by 22 publications

References 44 publications

Cell–Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation

Cell–Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation

Expression Changes and Impact of the Extracellular Matrix on Etoposide Resistant Human Retinoblastoma Cell Lines

Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels

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