Coulometric determination of the free organic acid content in wild-rose fruit

Абдуллина, С. Г.; Агапова, Н. М.; Лира, О. А.; Хазиев, Р. Ш.; Kodryanu, N. P.

doi:10.1007/s11094-011-0617-8

Cited by 2 publications

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“…It is very promising to determine the total OA content in the fruits of mountain ash, wild rosehips (pharmacopoeia species) and European dogwood by electrogenerated hydroxide ions. 19,20 It is established that malic acid interacts with electrogenerated hydroxide ions quickly and quantitatively in a 1: 2 ratio. The similarity of the curves of titration of malic acid and aqueous extract from rosehips suggests that in the aqueous extract, the amount of OA is titrated under the conditions found (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Sergunova¹,

Sorokina²,

Марахова³

2019

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Background: Organic acids (malic, citric, tartaric, oxalic, acetic, formic, isovaleric, ascorbic acids) make up a large group of biologically active substances and play an important role in plant and human metabolism. They are found in large quantities in the fruits of Rosaceae family medicinal plants that included in State Pharmacopoeia of the Russian Federation. Standardization of crude herbal drugs containing organic acids by modern physicochemical methods is a high-priority task. Materials and methods: The determination of total organic acids amount was carried out in aqueous extracts from different fruits of Rosaceae family plants by galvanostatic coulometry and potentiometry methods. Galvanostatic coulometry was performed with the help of the "Expert-006" coulometer with a current of 5 mA (integrated pH meter). Iodine as an electrogenerated titrant was used for ascorbic acid determination; electro generation of hydroxide ions was carried out for determination of total organic acids amount. A potentiometer "Aquilon pH-410" with attached glass and silver chloride electrodes was used for potentiometric determination of total organic acids amount. Individual organic acids have been determined by reverse-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UV) method. The following conditions were established: Gilson HPLC system, Alltech OA-1000 Organic Acids (6.5×300 mm, 9 μm) chromatography column, a gradient elution mode, component A of the mobile phase is 98% (0.1% phosphoric acid, 10 mM KH 2 PO 4 , solution in water) with 2% acetonitrile, component B is acetonitrile, the eluent feed rate is 1 ml/min. Results: Modern physicochemical methods for the analysis of biologically active substances, organic acids, for quality control of crude herbal drugs and medicinal herbal preparations, are developed and discussed. The optimal conditions for the qualitative and quantitative organic acid analysis are selected and described taking into account modern pharmacopoeial requirements. Conclusion: Galvanostatic coulometry and potentiometry methods, as well as RP-HPLC-UV, can be successfully used in the quality control of crude herbal drugs and medicinal herbal preparations, specifically fruits of Rosaceae family plants. Development and validation of analytical methods for monitoring the content of this BAS group is an important research area in the pharmacopoeial standardization of crude herbal drugs.

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Section: Resultsmentioning

confidence: 99%

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Sergunova¹,

Sorokina²,

Марахова³

2019

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“…Ascorbic acid content was determined by titration with electrically generated iodine. The titration end point was determined by amperometrically [21].…”

Section: Determination Of Bas Contentmentioning

confidence: 99%

Influence of Conservation Method (Freezing and Drying) on Composition and Content of Biologically Active Substances in Rosaceae Fruits (Crude Herbal Drugs)

Sergunova¹

2019

Asian J Pharm Clin Res

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Objective: Now, five crude herbal drugs (CHD) of fruit morphological group (fruits) related to Rosaceae family included in the Russian State Pharmacopoeia XI and XIII editions: Fructus Rosae (Rosa cinnamomea L. and other species), Fructus Crataegi (Crataegus sanguinea Pall. and other species), Fructus Sorbi (Sorbus aucuparia L.), Fructus Rubi (Rubus idaeus L.), and Fructus Aroniae (Aronia melanocarpa [Michx.] Elliott).The aim of this study was to investigate the effect of freezing and oven drying on the composition and content of individual groups of biologically active substances (BAS) in Rosaceae fruits.Methods: The groups of BAS were investigated by UV spectrophotometry (flavonoids and anthocyanins), galvanostatic coulometry titration (organic acids), gravimetry (polysaccharides), and titration with potassium permanganate (tannins).Results: The relation between content of BAS (total free organic acids, total flavonoids, total anthocyanins, total tannins, and total polysaccharides) and fruits conservation method was determined. In frozen fruits total polysaccharides, total free organic acids, ascorbic acid content decreases by 5–7%, total flavonoids and total tannins content - 10–15%, total anthocyanins content - 25–30%. Fruits oven drying at 60–80°C leads to more significant losses of total anthocyanins, ascorbic acid, and total free organic acids.Conclusion: The study of BAS stability in frozen and dried fruits was lasting for 12 months. The duration of shelf life for frozen fruits is recommended. Freezing as a conservation method allows to preserve more BAS in Rosaceae fruits. Frozen CHD can be proposed as an alternative to fresh and dried СРВ for the preparation of allopathic and homeopathic medicines.

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Coulometric determination of the free organic acid content in wild-rose fruit

Cited by 2 publications

References 1 publication

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Influence of Conservation Method (Freezing and Drying) on Composition and Content of Biologically Active Substances in Rosaceae Fruits (Crude Herbal Drugs)

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