Introduction
Gastric cancer occurred in China and even the whole East Asia with high incidence. The objective of this study was to investigate the role of
IL-17
in gastric cancer cells mediated by
LCN2
binding to
SLPI
.
Methods
The expression of
LCN2
and
SPLI
in gastric cancer cells and transfection effects were confirmed by RT-qPCR analysis. The proliferation, clone formation ability, invasion, migration, apoptosis, and cell cycle of gastric cancer cells were in turn detected by CCK-8 assay, clone formation assay, transwell assay, wound healing assay, and flow cytometry analysis. The combination between
LCN2
and
SLPI
was determined by co-immunoprecipitation assay. The expression of
Caspase-3, Bcl-2, cyclinB1, cyclinD1, MMP9,
and
SLPI
in gastric cancer cells was detected by Western blot analysis.
Results
LCN2
and
SPLI
exhibited the highest levels in AGS cells, and thus AGS cells were selected for the next experiments. Down-regulation of
LCN2
suppressed the proliferation and clone formation ability of AGS cells treated with
IL-17
.
IL-17
promoted the invasion and migration of AGS cells, which was partially reversed by the down-regulation of
LCN2
. Down-regulation of
LCN2
mediated by
IL-17
promoted apoptosis and suppressed the cell cycle of AGS cells.
Discussion
Down-regulation of
LCN2
mediated by
IL-17
suppressed the proliferation and suppressed the migration and invasion and cell cycle of gastric cancer cells by targeting
SLPI
.