Could S-Monovette Serum Gel Tubes be Used for Clinical Chemistry Analysis Instead of Serum Separator Tube II Advance?

Cat, Abdulkadir; Uçar, Kamil Taha; Ayan, Nilhan Nurlu

doi:10.7754/clin.lab.2021.210841

Cited by 3 publications

(3 citation statements)

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“…For serum sampling, blood was collected after 10 min seated in 9 ml tubes (Sarstedt S-Monovette ® Serum-Gel 9 ml, Sarstedt AG, Nümbrecht, Germany), considered equivalent from a clinical perspective to the BD Vacutainer ® SST II Plus (EU ref 367,955) and proposed in the WADA blood collection guidelines. 27 Venipuncture was realized with a 21G short manifold butterfly needle inserted into an antecubital vein (Sarstedt Safety-Multifly ® , Sarstedt AG, Nümbrecht, Germany). Serum samples were centrifuged (Z326K Centrifuge, Hermle Labortechnik, Wehingen, Germany) for 10 min at 2500 rpm.…”

Section: Sampling Methodsmentioning

confidence: 99%

“…Participants reported to the lab having avoided any physical exercise in the 2 h preceding the sampling and were asked to fill a disposable cup with their urine (Sarstedt 100 ml sterile containers, Sarstedt AG, Nümbrecht, Germany), and urine specific gravity was determined using a handheld refractometer (Pen‐Urine SG, Atago, Fukui City, Japan). For serum sampling, blood was collected after 10 min seated in 9 ml tubes (Sarstedt S‐Monovette® Serum‐Gel 9 ml, Sarstedt AG, Nümbrecht, Germany), considered equivalent from a clinical perspective to the BD Vacutainer® SST II Plus (EU ref 367,955) and proposed in the WADA blood collection guidelines 27 . Venipuncture was realized with a 21G short manifold butterfly needle inserted into an antecubital vein (Sarstedt Safety‐Multifly®, Sarstedt AG, Nümbrecht, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Variability of the urinary and blood steroid profiles in healthy and physically active women with and without oral contraception

Basile

Salamin

Krumm

et al. 2022

Drug Testing and Analysis

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The steroidal module of the athlete biological passport (ABP) targets the use of pseudo‐endogenous androgenous anabolic steroids in elite sport by monitoring urinary steroid profiles. Urine and blood samples were collected weekly during two consecutive oral contraceptive pill (OCP) cycles in 15 physically active women to investigate the low urinary steroid concentrations and putative confounding effect of OCP. In urine, testosterone (T) and epitestosterone (E) were below the limit of quantification of 1 ng/ml in 62% of the samples. Biomarkers' variability ranged between 31% and 41%, with a significantly lesser variability for ratios (except for T/E [41%]): 20% for androsterone/etiocholanolone (p < 0.001) and 25% for 5α‐androstane‐3α,17β‐diol/5ß‐androstane‐3α,17β‐diol (p < 0.001). In serum, markers' variability (testosterone: 24%, androstenedione: 23%, dihydrotestosterone: 19%, and T/A4: 16%) was significantly lower than in urine (p < 0.001). Urinary A/Etio increased by >18% after the first 2 weeks (p < 0.05) following withdrawal blood loss. In contrast, serum T (0.98 nmol/l during the first week) and T/A4 (0.34 the first week) decreased significantly by more than 25% and 17% (p < 0.05), respectively, in the following weeks. Our results outline steroidal variations during the OCP cycle, highlighting exogenous hormonal preparations as confounder for steroid concentrations in blood. Low steroid levels in urine samples have a clear negative impact on the subsequent interpretation of steroid profile of the ABP. With a greater analytical sensitivity and lesser variability for steroids in healthy active women, serum represents a complementary matrix to urine in the ABP steroidal module.

show abstract

Section: Sampling Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Variability of the urinary and blood steroid profiles in healthy and physically active women with and without oral contraception

Basile

Salamin

Krumm

et al. 2022

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…For serum sampling, blood was collected after 10 min seated in 9mL tubes (Sarstedt S-Monovette® Serum-Gel 9 mL, Sarstedt AG, Nümbrecht, Germany), considered equivalent from a clinical perspective to the BD Vacutainer® SST II Plus (EU ref 367955) proposed in the WADA blood collection guidelines. 24 Venipuncture was realized with a 21G short manifold butterfly needle inserted into an antecubital vein (Sarstedt Safety-Multifly®, Sarstedt AG, Nümbrecht, Germany). Serum samples were centrifuged (Z326K Centrifuge, Hermle Labortechnik, Wehingen, Germany) for 10 minutes at 2500 rpm.…”

Section: Sampling Methodsmentioning

confidence: 99%

Variability of the urinary and blood steroid profiles in healthy women

Basile

Salamin

Krumm

et al. 2022

Preprint

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The steroidal module of the Athlete Biological Passport (ABP) targets the use of exogenous androgenous anabolic steroids (EAAS) in elite sport by monitoring urinary steroid profiles. Urine and blood samples were collected weekly during two consecutive OCP cycles (8 weeks) in 15 physically active women to investigate the low urinary steroid concentrations and putative confounding effect of OCP. In urine, testosterone (T) and/or epitestosterone (E) were below the limit of quantification of 1 ng/mL in 62% of the samples. Biomarkers' variability ranged between 31% and 41%, with a significantly lesser variability for ratios (except for T/E (41%)): 20% for androsterone/etiocholanolone (p < 0.001) and 25% for 5alpha-androstane-3lapha,17beta-diol/5beta-androstane-3,17beta-diol (p < 0.001). In serum, markers variability (testosterone: 24%, androstenedione: 23%, dihydrotestosterone: 19% and T/A4: 16%) was significantly lower than in urine (p < 0.001). Urinary A/Etio increased by > 18% after the first two weeks (p < 0.05) following blood loss. In contrast, serum T (0.98 nmol/L during the first week), and T/A4 (0.34 the first week) decreased significantly by more than 25% and 17% (p < 0.05), respectively in the following weeks. Our results outline steroidal variations during the OCP cycle, highlighting exogenous hormonal preparations as confounder for steroid concentrations in blood. Low steroid levels in urine samples have a clear detrimental impact on the subsequent interpretation of steroidal variations for the ABP. With a greater analytical sensitivity and lesser variability for steroids in healthy active women, serum represents a complementary matrix to urine in the ABP steroidal module.

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End-user verification results of two serum separator tubes for clinical chemistry analytes according to CLSI GP34-A and CLSI GP41-A6

Abusoglu,

Ecer,

Guven

et al. 2024

Scandinavian Journal of Clinical and Laboratory Investigation

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Could S-Monovette Serum Gel Tubes be Used for Clinical Chemistry Analysis Instead of Serum Separator Tube II Advance?

Cited by 3 publications

References 0 publications

Variability of the urinary and blood steroid profiles in healthy and physically active women with and without oral contraception

Variability of the urinary and blood steroid profiles in healthy and physically active women with and without oral contraception

Variability of the urinary and blood steroid profiles in healthy women

End-user verification results of two serum separator tubes for clinical chemistry analytes according to CLSI GP34-A and CLSI GP41-A6

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