Could cryopreserved human semen samples be stored at -80°C?

Vaz, Carlos R; Lamim, Tamara; Salvador, Rafael Alonso; Batschauer, A.P.B.; Amaral, Vera Lucia Lângaro; Til, David

doi:10.5935/1518-0557.20180016

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…It has been reported in dog and mouse that the use of ultra‐freezer at −80°C was as effective as LN in preserving frozen‐thawed sperm fertility for at least 45 days and 1 year respectively (Pezo et al., 2017; Raspa et al., 2018). On the contrary, this storage temperature is supposed to be detrimental to cryopreserved human semen samples throughout 96 hr of storage (Vaz et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Successful short‐term sperm cryopreservation in brown‐marbled grouper (Epinephelus fuscoguttatus) with the utility of ultra‐freezer (−80°C)

Yang

Chen

Fan

et al. 2022

Reprod Domestic Animals

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Production of cryopreserved semen in fish generally requires liquid nitrogen (LN), which is not always easily available in remote areas. To reduce reliance on LN, the aim of the present study was to evaluate whether electric freezer could be a feasible LNfree alternative to cryopreserve brown-marbled grouper sperm. After loading, semen straws were put directly into freezers (−30 or −80°C) for freezing and then transferred to LN for storage. Compared with the conventional LN vapour freezing (straws were put horizontally 3 cm above the surface of LN), there was a significant reduction in all tested post-thaw sperm quality parameters in samples frozen at −30°C for 10 min, including kinetic parameters (total motility: 85.0% vs. 48.6%), viability (84.7% versus 51.7%), high mitochondrial membrane potential (86.4% vs. 63.7%), ATP content (106.9 nM/10 9 cells vs. 72.9 nM/10 9 cells) and hatching rate (86.3% vs. 45.7%), accompanied with an increasing lipid peroxidation level (MDA content: 11.9 nM/10 9 cells vs. 4.9 nM/10 9 cells). In contrast, frozen with −80 °C ultra-freezer (10 min or 12 hr) produced similar sperm quality parameters to those using LN, except that temporary storage (12 hr) at −80°C yielded lower average path velocity. In conclusion, this study confirmed that −80°C ultra-freezer is an effective alternative to LN for sperm freezing in brown-marbled grouper.

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Section: Discussionmentioning

confidence: 99%

Successful short‐term sperm cryopreservation in brown‐marbled grouper (Epinephelus fuscoguttatus) with the utility of ultra‐freezer (−80°C)

Yang

Chen

Fan

et al. 2022

Reprod Domestic Animals

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“…Buna göre -80°C sonrası sperm motilite, vitalite ve mitokondriyal aktivitelerde zamanla anlamlı azalma gözlenmiştir. [52] Spermin saklandığı süreden ziyade dondurma ve çözme işlemlerinin sperm kalitesindeki azalmayla ilişkili olduğu düşünülmektedir. Sperm dondurma işleminde sadece motilite değil akrozom reaksiyonu, mitokondrial aktivite ve morfolojinin de bozulduğu görülmektedir.…”

Section: öZunclassified

Sperm dondurma: Güncel gelişmeler

Erdemır¹

2019

Androl Bul

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F ertilite için gerekli olan spermin bazı durumlarda sınırlı olarak elde edildiği ya da gelecekte azalacağı bilindiği için spermin gerektiğinde kullanılmak üzere dondurularak saklanması gündeme gelmiştir. Fertilite ile ilişkili mücadele ve gayretler çiftlerin ruh sağlığını bozduğu için bu stresi azaltabilmesi açısından da sperm dondurma işlemi büyük önem arzetmektedir. [1,2] İlk olarak 1940'lı yıllarda veteriner hekimler tarafından uygulanan sperm dondurma işlemi spermlerin-80°C ile-196°C arasında gerektiğinde tekrar kullanılmak üzere saklanmasını içermekte olup insanlarda 1950'li yıllardan beri yapılmaktadır. [3] Başlangıçta kullanımı sadece intrauterin inseminasyon (IUI) için sınırlı olan sperm dondurma ile ilişkili endikasyonlar günümüzde ol-ABSTRACT Sperm cryopreservation involves the cooling of semen samples and their storage at-196°C in liquid nitrogen. This technique has been used widely since the 1960s to treat couples with infertility. Cancer is the major indication for sperm cryopreservation. However currently, the scope of clinical indications used for sperm cryopreservation has expanded widely. In this context, sperm cryopreservation is used in patients with retrograde ejaculation, severe olgoospermia, metabolic disorders, spinal cord injury, cranial tumors. Cryopreservation process involves cooling, freezing, and thawing steps. Cryopreservation of sperm may negatively affect on membrane lipid composition, acrosome status, sperm motility, vitality and DNA damage. To prevent of this negative effects several cryoprotectants have been used. In general the pregnancy rates after sperm cryopreservation are changes between 12% and 35.2%. In this review current advances have been evaluated in the era of sperm cryopreservation.

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“…In 2012, Sanchez et al (2012) concluded that there were no significant differences in sperm progressive motility, the integrity of mitochondrial membrane potential (MMP) or DNA fragmentation for vitrified swim-up human sperm either at −196 °C under liquid nitrogen or at -80 °C. However, Vaz et al (2018) found storage of human sperm at −80 °C freezer up to 96 hours was detrimental to sperm viability. Therefore, the impact of −80 °C freezer on human sperm needs to be further explored.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Efficient Method to Cryopreserve Human Ejaculated and Testicular Spermatozoa in −80°C Freezer

Wang

Bai

et al. 2022

Front. Genet.

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Human autologous sperm freezing involves ejaculated sperm, and testicular or epididymal puncture sperm freezing, and autologous sperm freezing is widely used in assisted reproductive technology. In previous studies, researchers have tried to cryopreserve sperm from mammals (rats, dogs, etc.) using a −80°C freezer and have achieved success. It is common to use liquid nitrogen vapor rapid freezing to cryopreserve human autologous sperm. However, the operation of this cooling method is complicated, and the temperature drop is unstable. In this study, we compared the quality of human ejaculation and testicular sperm after liquid nitrogen vapor rapid freezing and −80°C freezing for the first time. By analyzing sperm quality parameters of 93 ejaculated sperm and 10 testicular sperm after liquid nitrogen vapor rapid freezing and −80°C freezing, we found reactive oxygen species (ROS) of sperm of the −80°C freezer was significantly lower than liquid nitrogen vapor rapid freezing. Regression analysis showed that progressive motility, ROS, and DNA fragmentation index (DFI) in post-thaw spermatozoa were correlated with sperm progressive motility, ROS, and DFI before freezing. For the freezing method, the −80°C freezer was positively correlated with the sperm progressive motility. Among the factors of freezing time, long-term freezing was negatively correlated with sperm progressive motility and ROS. Although freezing directly at −80°C freezer had a slower temperature drop than liquid nitrogen vapor rapid freezing over the same period, the curves of the temperature drop were similar, and slight differences in the freezing point were observed. Furthermore, there were no statistically significant differences between the two methods for freezing testicular sperm. The method of direct −80°C freezing could be considered a simplified alternative to vapor freezing for short-term human sperm storage. It could be used for cryopreservation of autologous sperm (especially testicular sperm) by in vitro fertilization centers.Clinical Trial Registration: (website), identifier (ChiCTR2100050190).

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Could cryopreserved human semen samples be stored at -80°C?

Cited by 3 publications

References 15 publications

Successful short‐term sperm cryopreservation in brown‐marbled grouper (Epinephelus fuscoguttatus) with the utility of ultra‐freezer (−80°C)

Successful short‐term sperm cryopreservation in brown‐marbled grouper (Epinephelus fuscoguttatus) with the utility of ultra‐freezer (−80°C)

Sperm dondurma: Güncel gelişmeler

A Simple and Efficient Method to Cryopreserve Human Ejaculated and Testicular Spermatozoa in −80°C Freezer

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