Could 68Ga-DOTA-UBI-29-41 Help Identify Chronic Osteomyelitis on PET/CT Images? A Pilot Study

Nogueira, Solange Amorim; de Barboza, Marycel F.; Dell’Aquila, Adriana M.; Santos, Durval C. B; Osawa, Akemi

doi:10.1097/rlu.0000000000004866

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References 6 publications

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“…This case is part of a pilot project, led by the co-authors of this study, which aims to confirm the presence of bacteria in patients with chronic osteomyelitis using PET/CT images with [ 68 Ga]Ga-DOTA-UBI 29-41 . In this study, from the seven cases studied, only one case was negative, because the patient was under antibiotic therapy [ 42 ]. These findings are consistent with a study that presented the first PET/CT images with [ 68 Ga]Ga-NOTA-UBI 29-41 in patients.…”

Section: Discussionmentioning

confidence: 99%

Radiosynthesis Standardization and Preclinical Assessment of the [68Ga]Ga-DOTA-Ubiquicidin29-41: A Translational Study Targeting Differential Diagnosis of Infectious Processes

Miranda,

Fuscaldi,

Mejia

et al. 2023

Pharmaceuticals

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Human bacterial infections significantly contribute to the increase in healthcare-related burdens. This scenario drives the study of novel techniques for the early and precise diagnosis of infectious processes. Some alternatives include Nuclear Medicine- and Molecular Imaging-based strategies. However, radiopharmaceuticals that are available for routine assessments are not specific to differentiating infectious from aseptic inflammatory processes. In this context, [68Ga]Ga-DOTA-Ubiquicidin29-41 was synthesized using an automated module and radiochemical; in vivo and in vitro studies were performed. The radiopharmaceutical remained stable in saline (up to 180 min) and in rodent serum (up to 120 min) with radiochemical purities > 99 and 95%, respectively. Partition coefficient and serum protein binding at 60 min were determined (−3.63 ± 0.17 and 44.06 ± 1.88%, respectively). Ex vivo biodistribution, as well as in vivo microPET/CT images in mice, showed rapid blood clearance with renal excretion and reduced uptake in other organs in Staphylococcus aureus-infected animals. Higher uptake was observed in the target as compared to the non-target tissue (p < 0.0001) at 60 min post administration. The presented in-human clinical case demonstrates uptake of the radiopharmaceutical by Staphyloccocus aureus bacteria. These results indicate the potential of [68Ga]Ga-DOTA-Ubiquicidin29-41 as a radiopharmaceutical that can be obtained in a hospital radiopharmacy for the diagnosis of infectious processes using PET/CT.

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Section: Discussionmentioning

confidence: 99%