Cotton Plants Expressing a Hemipteran-Active Bacillus thuringiensis Crystal Protein Impact the Development and Survival of Lygus hesperus (Hemiptera: Miridae) Nymphs

Baum, James A.; Sukuru, Uma R.; Penn, S. R.; Meyer, Steven E.; Subbarao, Shubha; Shi, Xiaohong; Flasiński, Stanisław; Heck, Gregory R.; Brown, Robert D.; Clark, Thomas L.

doi:10.1603/ec11207

Cited by 72 publications

(67 citation statements)

References 31 publications

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“…The relatively low toxicity of Bt toxins against hemipteran pests has thus far prevented their application for management of these sap-sucking pests (4) although a Cry toxin with potential for use against Lygus hesperus has now been identified (24). We have demonstrated the use of a synthetic peptide to increase toxin binding to the aphid gut.…”

Section: Discussionmentioning

confidence: 99%

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Chougule

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Although transgenic crops expressing Bacillus thuringiensis (Bt) toxins have been used successfully for management of lepidopteran and coleopteran pest species, the sap-sucking insects (Hemiptera) are not particularly susceptible to Bt toxins. To overcome this limitation, we demonstrate that addition of a short peptide sequence selected for binding to the gut of the targeted pest species serves to increase toxicity against said pest. Insertion of a 12-aa pea aphid gut-binding peptide by adding to or replacing amino acids in one of three loops of the Bt cytolytic toxin, Cyt2Aa, resulted in enhanced binding and toxicity against both the pea aphid, Acyrthosiphon pisum, and the green peach aphid, Myzus persicae. This strategy may allow for transgenic plant-mediated suppression of other hemipteran pests, which include some of the most important pests of global agriculture.aphid management | biotechnology | insect resistance

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Section: Discussionmentioning

confidence: 99%

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Chougule

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Los datos del crecimiento micelial y porcentaje de inhibición se obtuvieron de la forma mencionada en el punto anterior. (Baum et al, 2012;Berry et al, 2012). The mortality assessment was performed every 24 h for five days after the start of the experiment.…”

Section: Bioensayos De Antagonismo In Vitrounclassified

“…Cada tratamiento se integró con 30 ninfas. Cabe mencionar que las bacterias B. thuringiensis y L. sphaericus se evaluaron con base en la actividad insecticida demostrada en trabajos previos (Baum et al, 2012;Berry et al, 2012). La evaluación de la mortalidad se realizó cada 24 h durante los cinco días posteriores del inicio del experimento.…”

Section: Bacterial Growth Kinetics In Two Culture Mediaunclassified

“…Otras investigaciones han mostrado que las rizobacterias bacteria on the weight of the nymph 4 to the nymph 5 regarding the control ( (Baum et al, 2012). This means that there may be toxic proteins against bedbugs, but we have to investigate how such proteins interact with the digestive tract of bedbugs.…”

Section: Mortality and Weight Reduction Of L Zonatus By The Effect Omentioning

confidence: 99%

“…Por tal razón, las toxinas de B. thuringiensis no interaccionaron con el tracto digestivo del insecto; sin embargo, recientemente se ha logrado expresar una proteína insecticida de 35 kDa de dicha bacteria que afecta la supervivencia y el desarrollo de la chinche Lygus hesperus (Knight) en plantas transgénicas de algodón (Baum et al, 2012). Lo anterior significa que pueden existir proteínas tóxicas contra chinches, pero se tiene que investigar la manera de que tales proteínas interaccionen con el tracto digestivo de las chinches.…”

Section: Mortality and Weight Reduction Of L Zonatus By The Effect Ounclassified

See 2 more Smart Citations

Activity of rhizobacteria of Jatropha curcas against Fusarium verticillioides and Leptoglossus zonatus

Hernández-Guerra¹,

Castrejón-Gómez²,

Valle³

et al. 2016

rchscfa

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First transgenic trait for control of plant bugs and thrips in cotton

Akbar

Gowda

Ahrens

et al. 2018

Pest Management Science

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BACKGROUND Plant bugs (Lygus spp.) and thrips (Thrips spp.) are two of the most economically important insect pest groups impacting cotton production in the USA today, but are not controlled by current transgenic cotton varieties. Thus, seed or foliar‐applied chemical insecticides are typically required to protect cotton from these pest groups. Currently, these pests are resistant to several insecticides, resulting in fewer options for economically viable management. Previous publications documented the efficacy of transgenic cotton event MON 88702 against plant bugs and thrips in limited laboratory and field studies. Here, we report results from multi‐location and multi‐year field studies demonstrating efficacy provided by MON 88702 against various levels of these pests. RESULTS MON 88702 provided a significant reduction in numbers of Lygus nymphs and subsequent yield advantage. MON 88702 also had fewer thrips and minimal injury. The level of control demonstrated by this transgenic trait was significantly better compared with its non‐transgenic near‐isoline, DP393, receiving insecticides at current commercial rates. CONCLUSION The level of efficacy demonstrated here suggests that MON 88702, when incorporated into existing IPM programs, could become a valuable additional tool for management of Lygus and thrips in cotton agroecosystems experiencing challenges of resistance to existing chemical control strategies. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Cotton Plants Expressing a Hemipteran-Active Bacillus thuringiensis Crystal Protein Impact the Development and Survival of Lygus hesperus (Hemiptera: Miridae) Nymphs

Cited by 72 publications

References 31 publications

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Activity of rhizobacteria of Jatropha curcas against Fusarium verticillioides and Leptoglossus zonatus

First transgenic trait for control of plant bugs and thrips in cotton

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