Cotton Genomics

Paterson, Andrew H.

doi:10.1007/978-3-642-04796-1_3

Cited by 1 publication

(3 citation statements)

References 119 publications

(112 reference statements)

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“…For many crops, the generation of stable transgenic plants is largely limited to a few genetic backgrounds. In cotton transformation, a persistent challenge has been the genotype specificity of cotton somatic embryogenesis, which is the most efficient transformation method (Trolinder, 2009; Paterson, 2010). Cotton transformation is limited to highly embryogenic cultivars, such as Coker 312 and Coker 5110 (Trolinder, 2009; Paterson, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…In cotton transformation, a persistent challenge has been the genotype specificity of cotton somatic embryogenesis, which is the most efficient transformation method (Trolinder, 2009; Paterson, 2010). Cotton transformation is limited to highly embryogenic cultivars, such as Coker 312 and Coker 5110 (Trolinder, 2009; Paterson, 2010). For utilization of VIGS as a tool for cotton gene function studies and functional genomics, the ability to silence genes readily in various genotypes is desirable.…”

Section: Resultsmentioning

confidence: 99%

“…, 2000). Although such technologies are being developed in cotton in order to understand the features that make cotton unique (Paterson, 2010), they have not yet been widely and successfully deployed in cotton functional genomics due to the technical challenge of transformation, long growth cycle and large genome size. In addition, these functional genomic approaches have certain drawbacks, such as the large populations required to disrupt a gene of interest, gene targeting bias and complications of multiple mutations or insertions, lack of obvious phenotypes due to the presence of large gene families, gene duplication and polyploidy, and lethality caused by complete loss of function (Borevitz and Ecker, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt

et al. 2011

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SUMMARYCotton is an important cash crop worldwide, and is a significant source of fiber, feed, foodstuff, oil and biofuel products. Considerable effort has been expended to increase sustainable yield and quality through molecular breeding and genetic engineering of new cotton cultivars. Given the recent availability of the whole-genome sequence of cotton, it is necessary to develop molecular tools and resources for large-scale analysis of gene functions at the genome-wide level. We have successfully developed an Agrobacterium-mediated virusinduced gene silencing (VIGS) assay in several cotton cultivars with various genetic backgrounds. The genes of interest were potently and readily silenced within 2 weeks after inoculation at the seedling stage. Importantly, we showed that silencing GhNDR1 and GhMKK2 compromised cotton resistance to the infection by Verticillium dahliae, a fungal pathogen causing Verticillium wilt. Furthermore, we developed a cotton protoplast system for transient gene expression to study gene functions by a gain-of-function approach. The viable protoplasts were isolated from green cotyledons, etiolated cotyledons and true leaves, and responded to a wide range of pathogen elicitors and phytohormones. Remarkably, cotton plants possess conserved, but also distinct, MAP kinase activation with Arabidopsis upon bacterial elicitor flagellin perception. Thus, using gene silencing assays, we have shown that GhNDR1 and GhMKK2 are required for Verticillium resistance in cotton, and have developed high throughput loss-of-function and gain-of-function assays for functional genomic studies in cotton.

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