Cotransfer of two linked human genes into cultured mouse cells.

Abstract

Two linked human genes which code for the expression of cytosol thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7

Chromosome-mediated gene transfer between closely realted strains of cultured mouse cells.

“…The physical nature of the unstable material is not known; from other experiments, it is known to correspond to a chromosomal fragment so small that two closely linked genes (coding for thymidine kinase and galactokinase) are cotransferred only 25% of the time (5). Loss of the fragment might result from failure to replicate, or failure to undergo effective spindle attachment (nondisjunction).…”

“…The transfer of genes from one type of cultured mammalian cell to another by means of isolated metaphase chromosomes has been reported by several groups of investigators (1)(2)(3)(4)(5). In all cases, the experimental design has been the classical one of microbial genetics: a mutant line of recipient cells is exposed to wild-type donor genetic material and plated in a selective medium, such that clones can arise only from cells of two types: those in which the mutant phenotype has reverted, or those in which the corresponding wild-type donor gene has been taken up and expressed.…”

Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.

“…As has been found by others (1,(3)(4)(5)(6)(7), the chromosome fragments tend to be lost on culture of recipient cells, in the absence of selection, although stable transferents have also been isolated. Evidence is also presented that the chromosome fragments are integrated into different chromosome size classes in different transferent clones.…”

Unstable transformation of mouse 3T3 cells by transfection with DNA from normal human lymphocytes.

“…Phenotypic stabilization due to extended selection has been reported after DNA-or chromosome-mediated gene transfer (Degnen et al, 1976;Willecke et al, 1976Willecke et al, , 1979Klobutcher et al, 1981;Scangos et al, 1981) and has been shown to be due to integration of the transgenomes into recipient chromosomes (Perucho et al, 1980;Robins et al, 1981). Recently, Murray et al (1983) showed that a transferred transforming gene was segregated from transformants when the selection pressure was lifted.…”