Cost of transport is increased after cold exposure in<i>Monodelphis domestica</i>: training for inefficiency

Schaeffer, Paul J.; Villarin, Jason J; Pierotti, David J.; Kelly, Daniel P.; Lindstedt, Stan L.

doi:10.1242/jeb.01703

Cited by 24 publications

(20 citation statements)

References 48 publications

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“…thereby indicating an increase in muscle energetics (Schaeffer et al 2005). The concordant upregulation of the muscle-specific UCP3 implied a molecular explanation for thermogenic processes seen in M. domestica.…”

Section: Introductionmentioning

confidence: 77%

“…Previously, the lack of BAT in marsupials led to suggestions of a thermogenic role for skeletal muscle (Kabat et al 2003;May 2003;Schaeffer et al 2003Schaeffer et al , 2005. The upregulation of UCP3 in Monodelphis domestica in response to cold mimics the regulation of UCPl, suggesting that UCP3 may uncouple myotubular mitochondria and produce heat, thereby compensating for the lack of BAT.…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial Proton Conductance in Skeletal Muscle of a Cold‐Exposed Marsupial, Antechinus flavipes, Is Unlikely to Be Involved in Adaptive Nonshivering Thermogenesis but Displays Increased Sensitivity toward Carbon‐Centered Radicals

Jastroch

Withers²,

Stoehr³

et al. 2009

Physiological and Biochemical Zoology

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The organs and molecular mechanisms contributing to adaptive thermogenesis in marsupials are not known because some species apparently lack brown adipose tissue (BAT). The increased oxidative capacity and presence of uncoupling protein 3 (UCP3) in skeletal muscle led to speculations on whether uncoupled respiration sustains endothermy in the cold, as found for BAT. Here, we investigated the role of mitochondrial proton conductance in the small Australian marsupial Antechinus flavipes during cold exposure. Although there was a tendency toward higher oxidative capacity in skeletal muscle, indicating metabolic adjustments to the cold, we observed no change in basal proton conductance of isolated myotubular and liver mitochondria. In eutherians, 4-hydroxynonenal (HNE) is an activator of mitochondrial uncoupling mediated by UCP3 and ANT (adenine nucleotide translocase). In the marsupial A. flavipes, proton conductance in myotubular mitochondria could be induced by HNE selectively in the cold-acclimated group. Induced uncoupling activity could be attributed to the ANT as judged by inhibition with carboxyatractylate, while GDP, a putative inhibitor of rodent UCP3, had no detectable effects on marsupial UCP3. In contrast to previous expectations, basal proton conductance in the myotubular mitochondria of marsupials does not contribute to adaptive thermogenesis, as found for eutherian BAT. Increased sensitivity of proton conductance to HNE by the ANT suggests a greater requirement for mild uncoupling activity that may convey protection from lipid peroxidation and mitigate reactive oxygen species production during cold stress.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Mitochondrial Proton Conductance in Skeletal Muscle of a Cold‐Exposed Marsupial, Antechinus flavipes, Is Unlikely to Be Involved in Adaptive Nonshivering Thermogenesis but Displays Increased Sensitivity toward Carbon‐Centered Radicals

Jastroch

Withers²,

Stoehr³

et al. 2009

Physiological and Biochemical Zoology

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“…Investigations into the evolutionary causes of this observed interspecific variation in terrestrial NCOT is likely to be fruitful. Possible correlates of variation in NCOT include climate and habitat characteristics: chronic cold exposure causes an increase in NCOT in goats Capra aegagrus hircus (Schaeffer et al, 2001) and short-tailed opossums Monodelphis domestica (Schaeffer et al, 2005), and climate has repeatedly been shown to be an important correlate of variation in energetics (White et al, 2008;Speakman and Król, 2010;White and Kearney, in press); and species from open habitats have higher maximum running speeds than those from less open habitats and arboreal species (Garland et al, 1988;White et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Comparative energetics of mammalian locomotion: Humans are not different

Halsey

White

2012

Journal of Human Evolution

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“…Reversible glutathionation of UCP3 in response to ROS and redox state provides a dynamic post-translational mechanism for regulating mitochondrial uncoupling. It also provides insight into findings from several studies that show that the UCP3 level is inversely related to exercise efficiency in humans and other animals (Mogensen et al, 2006;Schaeffer et al, 2005;Schrauwen et al, 1999).…”

Section: Uncoupling Factors In Musclementioning

confidence: 94%

Mitochondria to motion: optimizing oxidative phosphorylation to improve exercise performance

Conley

2016

Journal of Experimental Biology

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Mitochondria oxidize substrates to generate the ATP that fuels muscle contraction and locomotion. This review focuses on three steps in oxidative phosphorylation that have independent roles in setting the overall mitochondrial ATP flux and thereby have direct impact on locomotion. The first is the electron transport chain, which sets the pace for oxidation. New studies indicate that the electron transport chain capacity per mitochondria declines with age and disease, but can be revived by both acute and chronic treatments. The resulting higher ATP production is reflected in improved muscle power output and locomotory performance. The second step is the coupling of ATP supply from O 2 uptake (mitochondrial coupling efficiency). Treatments that elevate mitochondrial coupling raise both exercise efficiency and the capacity for sustained exercise in both young and old muscle. The final step is ATP synthesis itself, which is under dynamic control at multiple sites to provide the 50-fold range of ATP flux between resting muscle and exercise at the mitochondrial capacity. Thus, malleability at sites in these subsystems of oxidative phosphorylation has an impact on ATP flux, with direct effects on exercise performance. Interventions are emerging that target these three independent subsystems to provide many paths to improve ATP flux and elevate the muscle performance lost to inactivity, age or disease.

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Cost of transport is increased after cold exposure inMonodelphis domestica: training for inefficiency

Cited by 24 publications

References 48 publications

Mitochondrial Proton Conductance in Skeletal Muscle of a Cold‐Exposed Marsupial, Antechinus flavipes, Is Unlikely to Be Involved in Adaptive Nonshivering Thermogenesis but Displays Increased Sensitivity toward Carbon‐Centered Radicals

Mitochondrial Proton Conductance in Skeletal Muscle of a Cold‐Exposed Marsupial, Antechinus flavipes, Is Unlikely to Be Involved in Adaptive Nonshivering Thermogenesis but Displays Increased Sensitivity toward Carbon‐Centered Radicals

Comparative energetics of mammalian locomotion: Humans are not different

Mitochondria to motion: optimizing oxidative phosphorylation to improve exercise performance

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