In this paper, we have numerically and experimentally investigated the radiation mode of 45° tilted fiber grating (45° TFG). By introducing the axial distributions into the volume current method, we have established a three-dimension theoretical model to analyze spatial distributions of the radiation mode of 45° TFG, and measured the radiation mode spatial distributions in experiment. Both numerical and experimental results show that the radiation mode of 45° TFG exhibits an exponential reduction along the fiber axial direction, and a quasi-Gaussian profile along the radial direction of fiber. Additionally, we have also measured the detailed polarization dependent azimuthal intensity distributions of radiation modes of both sand p-polarized core modes. The degree of polarization (DOP) of radiation mode is around 99.886%, which is independent of the length and the polarization extinction ratio (PER) of the grating. Moreover, for the first time, we have experimentally observed a very weak p-polarized component existing in the radiation mode, which has matched well with the simulation results.