Cost-Effective Media for Production of Lactic Acid Bacteria Isolated from Poultry in Kuwait

Al-Mansour, Hamad; Alonaizi, Thnayan; Kishk, Mohamed; Hadi, Ahmad Mir; Al-Surrayai, Tahani; Alawadi, Mohamed; Varughese, Siby

doi:10.3923/ijps.2019.598.603

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…Studies on the potential of alternative media in nurturing microbial growth were conducted byNarh et al (2018), which used rice bran as an alternative medium, and Banagan et al(2012), which used coconut water. Additionally, the studies conducted byAlmansour et al (2019) andBurns et al (2008) showed comparable and even greater LAB growth in their formulated alternative media compared to the MRS medium.…”

mentioning

confidence: 89%

“…To address the economic issue that is persistently limiting the large-scale utilization of LAB, researchers conducted numerous studies on potential cost-effective alternative media for the cultivation of LAB. These include yeast extract, molasses, and tomato serum (Almansour et al 2019); corn steep liquor (Téllez-Luis et al 2003); whey and buttermilk (Burns et al 2008); de-lipidated egg yolk and yeast autolysate (Djeghri-Hocine et al 2007); papain-hydrolyzed whey (Ziadi et al 2010); cassava bagasse and sugarcane bagasse (John et al 2006); and ram horn (Kurbanoglu 2004). Most studies use molasses and corn steep liquor as nitrogen and carbohydrate sources, but there are also studies that explore the use of other cheap materials such as wheat flour hydrolysate, wheat bran, lactose, whey permeate, hydrolysates of fish viscera, wheat stillage (Lee et al 2013), ammonium bisulfate (Manzoor et al 2017, cheese whey and wheat germ extract (Papizadeh et al 2020), rice bran extract (Narh et al 2018), andcoconut water (Banagan et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Low-cost Media from Agro-industrial Wastes for the Cultivation and Metabolite Production of Lactic Acid Bacteria

Saguibo¹,

Pérez²,

Brion³

et al. 2022

Philipp J Sci

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Lactic acid bacteria (LAB) are the most well-documented, “generally regarded as safe” microorganisms used in food fermentation, cosmetics, and pharmaceuticals. Many probiotic strains are utilized for the production of beneficial metabolites, such as bacteriocins and lactic acid, which influence the preservation, organoleptic characteristics, and nutritional properties of food. However, these strains are usually cultured using complex and expensive media, which significantly contribute to the cost of the final product. Thus, this study aims to develop a low-cost alternative medium (LCAM) for the growth and metabolite production of industrially important LAB strains. After several preliminary experiments, an LCAM containing rice bran extract, waste coconut (mature) water, baker’s yeast extract, table sugar, and salts was subjected to a statistically-guided optimization study for growth support of a model LAB strain Lactobacillus plantarum BS. Based on the second-order response mathematical model, the optimized medium composition was 35% rice bran extract and 58% waste coconut water, 1% baker’s yeast extract, 1% table sugar, and 5% salt solution (LCAM A) plus a modified version without baker’s yeast and table sugar (LCAM B). Predicted values were acceptable at 95% (8.91 and 8.88 log CFU/mL) and highly comparable (102% relativity) with the commercial complex medium used as control. Both LCAM formulations were found to support the growth and metabolite production of different LAB genera (L. plantarum BS, Lactococcus lactis NCDO 497, Enterococcus faecalis M4-3, Streptococcus thermophilus BIOTECH 1555, and Pediococcus acidilactici BIOTECH 1068). The LCAM supported the metabolite production of these LAB strains comparable with the commercial medium but is 7.4–7.6-fold cheaper than the cheapest commercial medium. This significant cost reduction has a huge prospect on the large-scale utilization of LAB. Nevertheless, further studies are necessary to enhance the nutritive composition of LCAM for other genera of LAB.

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mentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Low-cost Media from Agro-industrial Wastes for the Cultivation and Metabolite Production of Lactic Acid Bacteria

Saguibo¹,

Pérez²,

Brion³

et al. 2022

Philipp J Sci

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“…So, it is important to develop alternative low-cost media for industrial applications like production of starter cultures and probiotics. Low-cost cultivation media from locally available materials such as mushrooms, tomatoes, corn, fish viscera hydrolysate, sweet potatoes, whey, buttermilk, pineapple peels, chick pea, cabbage, wheat, barley, and sugar beet molasses have been reported [ 8 – 18 ]. Sorghum is a low-cost cereal found in almost all parts of the world.…”

Section: Introductionmentioning

confidence: 99%

Sorghum Malt Extract as a Growth Medium for Lactic Acid Bacteria Cultures: A Case of Lactobacillus plantarum MNC 21

Byakika

Mukisa

Byaruhanga

2020

International Journal of Microbiology

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Cultivation of lactic acid bacteria cultures is vital for research and commercial production of fermented foods. However, the conventional growth media used are generally costly. Malt extracts from four sorghum varieties (SESO 1, SESO 3, Epuripur, and Eyera) were evaluated as alternative low-cost growth media for Lactobacillus plantarum MNC 21. Saccharified sorghum malt extracts were inoculated with 4 log cfu/mL MNC 21 and incubated at 30°C for 24 h. MRS broth was the reference medium. Microbial counts, pH, titratable acidity (TA), free amino nitrogen (FAN), and total sugars were measured. Maximum microbial counts in the extracts and MRS broth were 9 and 10 log cfu/mL, respectively. Maximum growth rate in the extracts was 0.7–0.9 log cfu/mL/h and 0.8 log cfu/mL/h in MRS broth. The final pH of the extracts was 3.5–3.6, with an overall increase in TA of 1.2% in Epuripur and 0.2% in other varieties. Final pH and TA of MRS broth were 4.1 and 1.3%, respectively. Total sugars dropped by 95.2% and FAN by 2.1% in MRS broth. In contrast, total sugars and FAN dropped by 5.6–9.1% and 24.9–32.7% respectively, in the extracts. Sorghum malt extracts can be adopted as alternative low-cost growth media for lactic acid bacteria cultures.

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Cost-Effective Media for Production of Lactic Acid Bacteria Isolated from Poultry in Kuwait

Cited by 2 publications

References 6 publications

Low-cost Media from Agro-industrial Wastes for the Cultivation and Metabolite Production of Lactic Acid Bacteria

Low-cost Media from Agro-industrial Wastes for the Cultivation and Metabolite Production of Lactic Acid Bacteria

Sorghum Malt Extract as a Growth Medium for Lactic Acid Bacteria Cultures: A Case of Lactobacillus plantarum MNC 21

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