Cost-effective and disposable label-free voltammetric immunosensor for sensitive detection of interleukin-6

Cancelliere, Rocco; Tinno, Alessio Di; Lellis, A. M. Di; Contini, G.; Micheli, Laura; Signori, Emanuela

doi:10.1016/j.bios.2022.114467

Cited by 30 publications

(16 citation statements)

References 36 publications

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“…For further immune score analysis, the immune-associated genes were extracted from differentially expressed genes ( Figure 4 C and Supplementary Table S2 ). The heatmap showed that chemokines and cytokines including CXCL5, IL12A, IL-6, IL19, CCL18, CCL25, and CCL7; immune regulators including CTLA4, AIM2, LAG3, PDCD1, ICOSLG, IDO1, and GSDMB; and human leukocyte antigens including HLA-DPA3, HLAD-DRB5, HLA-DOB, HLA-V, and HLA-DQB2 were significantly upregulated in the anthracycline-S group [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

Liu

Gao

et al. 2022

Biomolecules

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Anthracycline is a mainstay of treatment for breast cancer patients because of its antitumor activity. However, anthracycline resistance is a critical barrier in treating breast cancer. Thus, it is of great importance to uncover the molecular mechanisms underlying anthracycline resistance in breast cancer. Herein, we integrated transcriptome data, genetic alterations data, and clinical data of The Cancer Genome Atlas (TCGA) to identify the molecular mechanisms involved in anthracycline resistance in breast cancer. Two hundred and four upregulated genes and 1376 downregulated genes were characterized between the anthracycline-sensitive and anthracycline-resistant groups. It was found that drug resistance-associated genes such as ABCB5, CYP1A1, and CYP4Z1 were significantly upregulated in the anthracycline-resistant group. The gene set enrichment analysis (GSEA) suggested that the P53 signaling pathway, DNA replication, cysteine, and methionine metabolism pathways were associated with anthracycline sensitivity. Somatic TP53 mutation was a common genetic abnormality observed in the anthracycline-sensitive group, while CDH1 mutation was presented in the anthracycline-resistant group. Immune infiltration patterns were extremely different between the anthracycline-sensitive and anthracycline-resistant groups. Immune-associated chemokines and cytokines, immune regulators, and human leukocyte antigen genes were significantly upregulated in the anthracycline-sensitive group. These results reveal potential molecular mechanisms associated with anthracycline resistance.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

Liu

Gao

et al. 2022

Biomolecules

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“…In recent years, several methods including nanoparticle tracking analysis, western blotting, enzyme-linked immunosorbent assay, − fluorescence, colorimetric, electrochemical, surface-enhanced Raman scattering and CL assays were developed for exosome detection. Thereinto, the CL technique is worthy of attention for its advantages of a wide linear range, high sensitivity, and low costs. , However, as far as we know, only a few CL-based assays of exosomes have been developed. Moreover, most of them are based on labeling technology, which faces drawbacks of operational complexity, high costs, and slow speed.…”

mentioning

confidence: 99%

“…Thereinto, the CL technique is worthy of attention for its advantages of a wide linear range, high sensitivity, and low costs. 23,24 However, as far as we know, only a few CL-based assays of exosomes have been developed. Moreover, most of them are based on labeling technology, which faces drawbacks of operational complexity, high costs, and slow speed.…”

mentioning

confidence: 99%

N-(4-Aminobutyl)-N-ethylisopropanol and Co²⁺ Dual-Functionalized Core–Shell Fe₃O₄@Au/Ag Magnetic Nanomaterials with Strong and Stable Chemiluminescence for a Label-Free Exosome Immunosensor

Wang,

Shu,

Wang

et al. 2023

Anal. Chem.

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Recently, magnetic beads (MBs) are moving toward chemiluminescence (CL) functional magnetic nanomaterials with a great potential for constructing label-free immunosensors. However, most of the CL-functionalized MBs suffer from scarce binding sites, easy aggregation, and leakage of CL reagents, which will ultimately affect the analytical performance of immunosensors. Herein, by using core–shell Fe3O4@Au/Ag magnetic nanomaterials as a nanoplatform, a novel N-(4-aminobutyl)-N-ethylisopropanol (ABEI) and Co2+ dual-functionalized magnetic nanomaterial, namely, Fe3O4@Au/Ag/ABEI/Co2+, with strong and stable CL emission was successfully synthesized. Its CL intensity was 36 and 3.5 times higher than that of MB@ABEI-Au/Co2+ and ABEI and Co2+ dual-functionalized chemiluminescent MBs previously reported by our group, respectively. It was found that the excellent CL performance of Fe3O4@Au/Ag/ABEI/Co2+ could be attributed to the enrichment effect of the Au/Ag shell and the synergistic enhance effect of the Au/Ag shell and Co2+. A related CL mechanism has been proposed. Afterward, based on the intense and stable CL emission of Fe3O4@Au/Ag/ABEI/Co2+, a sensitive and effective label-free CL immunosensor for exosome detection was established. It exhibited excellent analytical performance with a wide detection range of 3.1 × 103 to 3.1 × 108 particles/mL and a low detection limit of 2.1 × 103 particles/mL, which were better than the vast majority of the reported CL immunosensors. Moreover, the proposed label-free CL immunosensor was successfully used to detect exosomes in human serum samples and enabled us to distinguish healthy persons and lung cancer patients. It has the potential to be a powerful tool for exosome study and early cancer diagnosis.

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“…23,24 Nevertheless, this study achieves the LOD required for IL-6 detection in a short test time comparable to those of recent electrochemical label-free This journal is © The Royal Society of Chemistry 2023 sensors. 27,28 This shows its potential as a device for POCT, and we intend to expand this sensor into a system using various specimens by optimizing structures of the membrane pore and graed polymer.…”

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confidence: 99%

Design of a highly sensitive and versatile membrane-based immunosensor using a Cu-free click reaction

et al. 2023

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Cost-effective and disposable label-free voltammetric immunosensor for sensitive detection of interleukin-6

Cited by 30 publications

References 36 publications

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

N-(4-Aminobutyl)-N-ethylisopropanol and Co²⁺ Dual-Functionalized Core–Shell Fe₃O₄@Au/Ag Magnetic Nanomaterials with Strong and Stable Chemiluminescence for a Label-Free Exosome Immunosensor

Design of a highly sensitive and versatile membrane-based immunosensor using a Cu-free click reaction

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Cost-effective and disposable label-free voltammetric immunosensor for sensitive detection of interleukin-6

Cited by 30 publications

References 36 publications

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

Comprehensive Analysis of Transcriptomics and Genetic Alterations Identifies Potential Mechanisms Underlying Anthracycline Therapy Resistance in Breast Cancer

N-(4-Aminobutyl)-N-ethylisopropanol and Co2+ Dual-Functionalized Core–Shell Fe3O4@Au/Ag Magnetic Nanomaterials with Strong and Stable Chemiluminescence for a Label-Free Exosome Immunosensor

Design of a highly sensitive and versatile membrane-based immunosensor using a Cu-free click reaction

Contact Info

Product

Resources

About

N-(4-Aminobutyl)-N-ethylisopropanol and Co²⁺ Dual-Functionalized Core–Shell Fe₃O₄@Au/Ag Magnetic Nanomaterials with Strong and Stable Chemiluminescence for a Label-Free Exosome Immunosensor