Four systemic, four non-systemic and two combined fungicides at different concentrations were tested in vitro through poison food technique against Aspergillus flavus (Link), a causal organism of afla rot of groundnut. All the fungicides with their respective concentration were found inhibitory to the radial growth of A. flavus. Among all the systemic fungicides the highest growth inhibition of 99.99 per cent was recorded with tebuconazole 25% EC at all concentration (100, 250 and 500 ppm) followed by carbendazim 50% WP at 500 ppm (99.99%), 250 ppm (85.64%) and 100 ppm (82.64%) and hexaconazole 5% EC at 500 ppm (99.99%), 250 ppm (84.75%) and 100 ppm (77.58%). Among non-systemic fungicides, the highest growth inhibition of 90.88, 86.01 and 81.19 per cent were recorded with mancozeb 75% WP at 1500, 1000 and 500 ppm concentration, respectively. Among the combined fungicides, the highest growth inhibition 99.99 per cent was recorded with Carbendazim 12% + Mancozeb 63% WP at concentrations of 1500, 1000 and 500 ppm, respectively.