Corynebacterium thomssenii sp. nov., a Corynebacterium with N/acetyl- -glucosaminidase activity from human clinical specimens

Abstract

A strain of a previously undescribed non-lipophilic coryneform bacterium was isolated from pleural fluids of a patient with chronic renal failure, stroke and pneumonia. Slow fermentative acid production from glucose, maltose and sucrose, and strong N-acetyl-p-glucosaminidase activity were the most characteristic features of the bacterium. Chemotaxonomic characterization unambiguously indicated that the organism belonged to the genus Corynebacterium. The results of comparative 165 rRNA gene sequence analysis revealed that the isolate represented a new species within the genus, for which the name Corynebacterium thomssenii sp. nov. is proposed. The type strain is DSM 44276.

Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra)

“…Amino acid and cell-wall sugar analyses were performed according to described procedures (Staneck & Roberts, 1974). Menaquinone and cellular fatty acid composition were determined as described by Zimmermann et al (1998).…”

Xylanibacterium ulmi gen. nov., sp. nov., a novel xylanolytic member of the family Promicromonosporaceae

“…Cell-wall sugar analysis was performed according to described procedures (Staneck & Roberts, 1974). Menaquinone and cellular fatty acid compositions were analysed as described by Zimmermann et al (1998). Peptidoglycan and cell-wall amino acids were determined as described by Schleifer (1985) and Schleifer & Kandler (1972).…”

Characteristics of Rare or Recently Described Corynebacterium Species Recovered from Human Clinical Material in Canada

“…The 16S rRNA gene sequences for 20 of 73 strains reported here have been deposited in GenBank and are further described in Table 1. Identification was based on characteristics described in standard texts (10,11) as well as for C. argentoratense (26), C. bovis (13,19), C. confusum (9), C. coyleae (17), C. durum (21,24), C. falsenii (29), C. glucuronolyticum (14), C. imitans (12), C. kroppenstedtii (3), C. mucifaciens (15), C. riegelii (16), C. simulans (31), C. sundsvallense (4), C. thomssenii (33), and a black-pigmented Corynebacterium taxon group (28). Where all strains from a single taxon group had been sequenced, the percent identity shown is the lowest value obtained, with others being higher with respect to percent identity to the GenBank accession number for the reference strain of the species.…”