Corynebacterium pseudotuberculosis biovar ovis: evaluación de la sensibilidad antibiótica in vitro

Gallardo, Adriana Alicia; Toledo, Rocío Andrea; Pasayo, Ramón A. González; Azevedo, Vasco; Robles, Carlos Alejandro; Paolicchi, Fernando Alberto; Belchior, Silvia Graciela Estevao

doi:10.1016/j.ram.2018.12.001

Cited by 8 publications

(8 citation statements)

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“…Performing resistance testing of isolates can provide guidance for the use of antibiotics. At present, there have been limited studies on antibiotic susceptibility of C. pseudotuberculosis in alpaca, with most research focusing on horse ( Foley et al, 2004 ; Rhodes et al, 2015 ), sheep ( Gallardo et al, 2019 ), and goat ( Connor et al, 2000 ; Abebe and Sisay, 2015 ) isolates. The minimum inhibitory concentration (MIC) of the antimicrobial agent showed no significant differences between the isolates from horses and cows compared to those from sheep and goats, except in the case of amikacin ( Costa et al, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

et al. 2023

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Corynebacterium pseudotuberculosis is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, C. pseudotuberculosis biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore technology. The three strains exhibited resistance to aztreonam, fosfomycin, and nitrofurantoin. The median lethal doses (LD50) of strains G1, S2 and BA3 in experimentally infected mice was 1.66 × 105 CFU, 3.78 × 105 CFU and 3.78 × 105 CFU, respectively. The sequencing of strain G1 resulted in the assembly of a chromosomal scaffold comprising 2,379,166 bp with a G + C content of 52.06%. Genome analysis of strain G1 revealed the presence of 48 virulence genes and 5 antibiotic resistance genes (ARGs). Comparative genomic analysis demonstrates a high degree of genetic similarity among C. pseudotuberculosis strains, in contrast to other Corynebacterium species, with a clear delineation between strains belonging to the two biovars (ovis and equi). The data of the present study contribute to a better understanding of the properties of C. pseudotuberculosis biovar equi strains and the potential risk they pose to alpacas and other livestock, as well as the necessity of ongoing surveillance and monitoring of infectious diseases in animals.

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Section: Discussionmentioning

confidence: 99%

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

et al. 2023

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“…According to the MIC values and EUCAST clinical breakpoints for Corynebacterium spp., antibiotic resistance of the C. pseudotuberculosis strains from our study was observed only in terms of benzylpenicillin (72.7%), and 30.3% of the strains were classified as intermediate to meropenem according to CLSI. Strains of C. pseudotuberculosis that are less sensitive or resistant to some β-lactam antibiotics have also been reported in samples originating from small ruminants in Spain [48], Kosovo [49] and Egypt [50]. Antibiotics for the treatment of CLA are used in the Czech Republic principally on small or hobby sheep and goat farms or in the case of valuable breeding animals.…”

Section: Phenotype Analysesmentioning

confidence: 99%

Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study

Markova,

Langova,

Babak

et al. 2024

Microorganisms

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Caseous lymphadenitis (CLA) is a worldwide disease of small ruminants caused by Corynebacterium pseudotuberculosis, a facultative intracellular pathogen that is able to survive and multiply in certain white blood cells of the host. In this study, 33 strains of C. pseudotuberculosis were isolated from sheep and goats suffering from CLA on nine farms in the Czech Republic. All these strains were tested for their antibiotic susceptibility, ability to form a biofilm and resistance to the effects of commonly used disinfectant agents. To better understand the virulence of C. pseudotuberculosis, the genomes of strains were sequenced and comparative genomic analysis was performed with another 123 genomes of the same species, including ovis and equi biovars, downloaded from the NCBI. The genetic determinants for the virulence factors responsible for adherence and virulence factors specialized for iron uptake and exotoxin phospholipase D were revealed in every analyzed genome. Carbohydrate-Active Enzymes were compared, revealing the presence of genetic determinants encoding exo-α-sialidase (GH33) and the CP40 protein in most of the analyzed genomes. Thirty-three Czech strains of C. pseudotuberculosis were identified as the biovar ovis on the basis of comparative genome analysis. All the compared genomes of the biovar ovis strains were highly similar regardless of their country of origin or host, reflecting their clonal behavior.

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“…Esta é uma enfermidade contagiosa, cuja característica mais importante é a presença de granulomas em linfonodos internos e externos e em órgãos como pulmão, rim e fígado dos animais acometidos, caracterizando as duas formas clínicas da doença (SÁ et al, 2021) A LC apresenta grande importância devido aos consideráveis prejuízos econômicos, bem como à dificuldade do tratamento e a ausência de vacinas eficazes e seguras, o que justifica a necessidade de estudos mais aprofundados sobre a doença e seu agente etiológico (SOUZA, et al, 2011). (GALLARDO et al, 2019), ocorre a formação de biofilme bacteriano, uma barreira formada por uma cápsula de tecido fibroso que envolve a lesão, fazendo com que os antibióticos tenham uma baixa eficácia contra esse patógeno (BEZERRA et al, 2021). Além disso, a resistência deste patógeno aos antibióticos tem crescido nos últimos anos (EL DAMATY et al, 2023).…”

Section: Linfadenite Caseosaunclassified

Ciências Rurais em Foco - Volume 10

2023

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Modo de acesso: World Wide Web Inclui bibliografia 1. Agricultura 2. Meio Ambiente 3. Zootecnia 4. Ciências Agrárias I. REDIN, Ezequiel II. Título. CDD-630 Sônia Márcia Soares de Moura -CRB 6/1896 O conteúdo deste livro está licenciado sob a Licença de Atribuição Creative Commons 4.0. Com ela é permitido compartilhar o livro, devendo ser dado o devido crédito, não podendo ser utilizado para fins comerciais e nem ser alterada. O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos seus respectivos autores www.poisson.com.br

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Corynebacterium pseudotuberculosis biovar ovis: evaluación de la sensibilidad antibiótica in vitro

Cited by 8 publications

References 11 publications

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study

Ciências Rurais em Foco - Volume 10

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