Corynebacterium parvum enhances colonic cancer in dimethylhydrazine-treated rats

Cruse, J. P.; Lewin, M. R.; Clark, C. G.

doi:10.1038/bjc.1978.94

Cited by 17 publications

(2 citation statements)

References 18 publications

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“…Animals were allowed free access to tap water during an initial 2 weeks accli matization period, but thereafter were randomly allocated to one of three groups: Group D (n = 12) received DMH 40 mg/kg body weight/rat/week s.c. for 10 weeks plus fresh water ad libitum. The DMH was prepared as described previouly [7], Group DAX (n = 24) received DMH as above plus Aludrox 20 mg/kg body weight/rat/day in their drinking water. This dosage was selected to approximate, on a body weight basis, the therapeutic dosage of Aludrox used in a 70-kg man [3], Group AX (n = 12) received equivalent vol umes of saline subcutaneously for 10 weeks plus Aludrox as above in their drinking water.…”

Section: Methodsmentioning

confidence: 99%

“…They were weighed weekly and inspected daily for signs of illness or colonic disease. Moribund animals were isolated to prevent cannibalism and either died spontaneously or were pain lessly killed for humane reasons when they fulfilled objective criteria for terminal killing [7], Complete necropsies, including the selection of specimens for routine histological pro cessing and examination, were done on all animals. Colonic tumours were classified histo logically as carcinoma only if they demonstrated invasion through the muscularis mucosae, according to the criteria of .Uarson [13).…”

Section: Methodsmentioning

confidence: 99%

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Experimental Evidence against the Bile Salt Theory of Colon Carcinogenesis

Cruse¹,

Lewin²,

Ferulano³

et al. 1981

Eur Surg Res

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According to the bile salt theory of colon carcinogenesis, therapeutic agents which increase the delivery of bile salts to the large intestine may promote colon cancer. The possibility that aluminium hydroxide (Aludrox), a bile salt binding agent, might facilitate colon carcinogenesis in vivo was tested experimentally, using the dimethylhydrazine (DMH)-induced rat colon cancer model. 48 Wistar rats were randomly allocated to 1 of 3 groups, all fed the same standard diet. Two groups received a course of ten weekly DMH injections. One was allowed fresh drinking water ad libitum whilst the other received Aludrox in their drinking water. A third group received weekly saline injections plus Aludrox in their drinking water. After 1 year’s observation, there were no significant differences between the groups of DMH-injected rats given drinking water with or without Aludrox in respect of survival, necropsy incidence of primary or metastatic colon cancer, or in the total number of colon tumours per group. The results provide reassurance that Aludrox does not promote colon cancer and tend to contradict the bile salt theory of colon carcinogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Experimental Evidence against the Bile Salt Theory of Colon Carcinogenesis

Cruse¹,

Lewin²,

Ferulano³

et al. 1981

Eur Surg Res

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Experimental adenomas and carcinomas of the large intestine behave as distinct entities: Most carcinomas arisede novo in flat mucosa

Maskens

Dujardin-Loits

1981

Cancer

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Detailed histologic analyses were performed on tumors of the large intestine obtained in 152 dimethylhydrazine (DMH)-treated rats. Of a total 539 glandular neoplasms, 45 were benign; 494 (92%) were locally invasive; of which 222 (41%) were invading the muscularis propria. One-hundred-forty-one tumors were smaller than or equal to 3mm in diameter. Among those, 127 (90%) were invasive. In addition to macroscopic nodules, several appeared after longer latency periods than did serial sections of flat mucosa. The benign polyps usually appeared after longer latency periods than did carcinomas. A review of the literature indicates that in the majority of rat experiments most or all DMH-induced tumors were frequently reported. All these data constitute strong evidence that most experimental adenocarcinomas do arise de novo in flat mucosa, i.e., without a prior adenoma stage. However, most DMH-induced tumors in mice were reported to be adenomas, either alone or coexisting with carcinomas. It is suggested that "de novo arising carcinomas" and adenomatous polyps, which are both inducible by the same carcinogens, and which can coexist in some experimental systems, nonetheless constitute independent and distinct pathologic entities; they can be separated by genetic susceptibility.

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Inhibition of experimental colorectal cancer by razoxane (ICRF-159)

Gilbert¹,

Thompson²,

Slavin³

et al. 1984

British Journal of Surgery

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Large bowel cancer induced in rats by dimethylhydrazine (DMH) closely resembles human disease both histologically and clinically. In this study it has been used to assess the antimitotic drug razoxane (ICRF-159). Dimethylhydrazine induced benign colorectal tumours after 20 weeks and adenocarcinomas from 30 weeks. Razoxane was given from week 25 until the end of experiment (week 35) to see if this drug could inhibit the development of tumours. Animals were randomly allocated to four groups: DMH + razoxane (26), DMH + control (26), control + razoxane (25), control + control (25). There were fewer malignant colorectal tumours in rats receiving razoxane than in the controls (10 vs. 24; P = 0.04). Some animals developed malignant tumours of the small intestine as well as the large bowel and the number of malignant tumours for the whole intestinal tract was also reduced in animals receiving razoxane (15 vs. 32; P = 0.025). This study demonstrates inhibition of the development of malignant tumours in rats by razoxane. This finding may have relevance to colorectal cancer in man where razoxane has been used in disseminated disease and as an adjuvant to surgery.

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Corynebacterium parvum enhances colonic cancer in dimethylhydrazine-treated rats

Cited by 17 publications

References 18 publications

Experimental Evidence against the Bile Salt Theory of Colon Carcinogenesis

Experimental Evidence against the Bile Salt Theory of Colon Carcinogenesis

Experimental adenomas and carcinomas of the large intestine behave as distinct entities: Most carcinomas arisede novo in flat mucosa

Inhibition of experimental colorectal cancer by razoxane (ICRF-159)

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