Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis

Bernard, Kathryn; Pacheco, Ana Luisa; Loomer, Courtney; Burdz, Tamara; Wiebe, Deborah; Huynh, Chris; Kaplen, Brynn; Olson, Adam B.; Cnockaert, Margo; Eguchi, Hiroshi; Kuwahara, Tomomi; Nakayama‐Imaohji, Haruyuki; Shiota, Hiroshi; Boudewijns, Michaël; Hoecke, Frederik Van; Vandamme, Peter

doi:10.1099/ijsem.0.001059

Cited by 38 publications

(27 citation statements)

References 24 publications

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“…mast is a known skin commensal; however, its presence in the conjunctiva has also been described (Bernard et al, 2016). Our findings showed that C. mast can actively colonize ocular tissue and rather than maintaining its better known rod-like morphology, it appeared to take on a filamentous morphology similar to Corynebacterium spp.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study we identified Corynebacterium mastitidis ( C. mast ) as a candidate resident commensal and dissected the immunological interactions that led to an enhancement of host defense at the ocular surface. Using a mouse model, we demonstrated that C. mast , a commensal organism found in both humans and mice (Bernard et al, 2016; Eguchi et al, 2008; Kobayashi et al, 2015), was able to uniquely colonize the ocular surface and caused γδ T cells within the ocular mucosa to produce interleukin 17 (IL-17). This interaction resulted in the production and release of antimicrobial molecules into the tears and confered protection from invasive Candida albicans and Pseudomonas aeruginosa infection of the ocular surface.…”

Section: Introductionmentioning

confidence: 99%

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An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells

et al. 2017

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Summary Mucosal sites such as the intestine, oral cavity, nasopharynx, and vagina all have associated commensal flora. The surface of the eye is also a mucosal site, but proof of a living, resident ocular microbiome remains elusive. Here, we used a mouse model of ocular surface disease to reveal that commensals were present in the ocular mucosa and had functional immunological consequences. We isolated one such candidate commensal, Corynebacterium mastitidis and showed that this organism elicited a commensal-specific interleukin 17 response from γδ T cells in the ocular mucosa that was central to local immunity. The commensal-specfic response drove neutrophil recruitment and the release of antimicrobials into the tears, and protected the eye from pathogenic Candida albicans or Pseudomonas aeruginosa infection. Our findings provide direct evidence that a resident commensal microbiome exists on the ocular surface and identify the cellular mechanisms underlying its effects on ocular immune homeostasis and host defense.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells

et al. 2017

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“…Analysis was performed with Biotyper 4.2 software and compared with the Bruker Daltonics Biotyper database versions 7311, 7854 or 8468. Mass spectra profiles (MSPs) were prepared for all NML strains shown in Table 1 using methods outlined previously [24]. that data is summarized in Table S1, (available in the online version of this article).…”

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confidence: 99%

Description of Eikenella halliae sp. nov. and Eikenella longinqua sp. nov., derived from human clinical materials, emendation of Eikenella exigua Stormo et al. 2019 and emendation of the genus Eikenella to include species which are strict anaerobes

Bernard

Burdz

Wiebe

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, Kingella (HACEK) group genus Eikenella contained a single species, Eikenella corrodens, for many years. In November 2019, Eikenella exigua was described after recovery from a brain abscess and blood culture in Norway. Coincidentally, characterization of 22 Gram-negative bacteria resembling Eikenella from 17 Canadian patients had been underway. Seven isolates from five patients were conclusively identifiable as E. corrodens . One (NML 120819) was deemed to represent a species of the genus Eikenella most closely related to E. corrodens . Fourteen isolates had 97.6 to 98.8% similarities to E. corrodens by 16S rRNA gene sequencing, forming three distinct groups by genome analyses. The largest contained ten anaerobic isolates from eight patients recovered from blood, brain, bone and other abscesses; upon re-evaluation, this group was found to be most consistent with E. exigua . A second facultatively anaerobic clade consisted of two ocular isolates from one patient and a sinus isolate from a second patient. The third taxon consisted of a single strictly anaerobic blood culture isolate. The novel taxa, like E. corrodens , were poorly reactive biochemically and difficult to discern from each other phenotypically and chemotaxonomically, including by cellular fatty acids. MALDI-TOF (Bruker) and whole-genome sequencing were used to further characterize isolates. Draft genomes for the strains had similar DNA G+C contents (55.38–58.53 mol%) while sizes varied from 1.82 Mb to 2.54 Mb. We propose here emendations of the genus Eikenella and the species Eikenella exigua , as well as describing Eikenella halliae sp. nov. NML 130454T (=LMG 30894T=NCTC 14180T) and Eikenella longinqua sp. nov. NML 02-A-017T (=LMG 30896T=NCTC 14179T), on the basis of these findings.

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“…These close taxonomic relationships were then assessed by whole genome sequence (WGS) analysis. Each strain was subjected to whole genome sequencing using a paired-end Nextera library and the Illumina MiSeq platform, as outlined previously [6]. Contigs from strain NML120412 were further assembled into scaffolds using mate pair reads with SSpace version 2.2.0 as described previously [7].…”

mentioning

confidence: 99%

Corynebacterium godavarianum Jani et al. 2018 and Corynebacterium hadale Wei et al. 2018 are both later heterotypic synonyms of Corynebacterium gottingense Atasayar et al. 2017, proposal of an emended description of Corynebacterium gottingense Atasayar et al. 2017

Bernard

Pacheco

Burdz

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Seven strains of an unidentifiable Corynebacterium species recovered from blood cultures, urine or cerebrospinal fluid over 26 years, closest to but differentiated from Corynebacterium imitans by 16S rRNA gene and partial rpoB gene sequencing, were studied. In November 2017, Atasayar et al. described a blood culture isolate as Corynebacterium gottingense sp. nov., which had >99 % similarity by 16S rRNA gene sequencing to the Canadian strains. In January 2018, Jani et al. described Corynebacterium godavarianum sp. nov., recovered from the Godavari River, India, which also had >99 % similarity by 16S/rpoB sequencing to the Canadian strains and C. gottingense. In May 2018, Wei et al. described Corynebacterium hadale recovered from hadopelagic water; this too had >99 % similarity by 16S rRNA gene sequencing to C. gottingense , C. godavarianum and the Canadian strains. C. gottingense DSM 103494T and C. godavarianum LMG 29598T were acquired and whole genome sequencing was performed (not previously done). Results were compared with genomes from C. hadale (GenBank accession NQMQ01) and the Canadian isolates. We found that these ten genomes formed a single taxon when compared using digital DNA–DNAhybridization, average nucleotide identity using blastn and average amino acid identity criteria but exhibited some subtle biochemical and chemotaxonomic differences. Heuristically, we propose that C. godavarianum and C. hadale are later heterotypic synonyms of, and the Canadian isolates are identifiable as, C. gottingense . We provide an emended description of Corynebacterium gottingense Atasayar et al. 2017; genomes ranged from 2.48 to 2.69 Mb ( C. gottingense DSM 103494T, 2.62 Mb) with G+C content of 65.1–65.6 mol% (WGS), recovered from clinical and environmental sites.

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Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis

Cited by 38 publications

References 24 publications

An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells

An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells

Description of Eikenella halliae sp. nov. and Eikenella longinqua sp. nov., derived from human clinical materials, emendation of Eikenella exigua Stormo et al. 2019 and emendation of the genus Eikenella to include species which are strict anaerobes

Corynebacterium godavarianum Jani et al. 2018 and Corynebacterium hadale Wei et al. 2018 are both later heterotypic synonyms of Corynebacterium gottingense Atasayar et al. 2017, proposal of an emended description of Corynebacterium gottingense Atasayar et al. 2017

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