Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell

Wu, Chunyuan; Li, Zhuang; Zhou, Shungui; Li, Fangbai; He, Jun

doi:10.1099/ijs.0.020909-0

Cited by 80 publications

(47 citation statements)

References 32 publications

(28 reference statements)

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“…Microbial growth supported by iron reduction has been observed at pH values up to 11 (Ye et al 2004;Pollock et al 2007;Wu et al 2011) suggesting that the brown soil with a pH value >12 is a rather marginal environment for iron reducing bacteria. However an average pH value does not fully characterise the geochemical environment of a soil, where there may be microenvironments.…”

Section: Discussionmentioning

confidence: 97%

Biogeochemical Reduction Processes in a Hyper-Alkaline Leachate Affected Soil Profile

Burke

Mortimer

Palaniyandi

et al. 2012

Geomicrobiology Journal

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Hyperalkaline surface environments can occur naturally or because of contamination by hydroxide-rich wastes. The high pH produced in these areas has the potential to lead to highly specialised microbial communities and unusual biogeochemical processes. This paper reports an investigation into the geochemical processes that are occurring in a buried, saturated, organicrich soil layer at pH 12.3. The soil has been trapped beneath calcite precipitate (tufa) that is accumulating where highly alkaline leachate from a lime kiln waste tip is emerging to atmosphere. A population of anaerobic alkaliphilic bacteria dominated by a single, unidentified species within the Comamonadaceae family of -proteobacteria has established itself near the top of the soil layer. This bacterial population appears to be capable of nitrate reduction using electron donors derived from the soil organic matter. Below the zone of nitrate reduction a significant proportion of the 0.5N HCl extractable iron (a proxy for microbial available iron) is in the Fe(II) oxidation state indicating there is increasing anoxia with depth and suggesting that microbial iron reduction is occurring.

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Section: Discussionmentioning

confidence: 97%

Biogeochemical Reduction Processes in a Hyper-Alkaline Leachate Affected Soil Profile

Burke

Mortimer

Palaniyandi

et al. 2012

Geomicrobiology Journal

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“…nov (Wu et al 2010), and Geobacter metallireducens (Lovley et al 1996b). To the best of our knowledge, this study, for the first time reported a strain of P. aeruginosa could utilize AQDS as the sole electron acceptor for their growth under anaerobic conditions.…”

Section: Effect Of Electron Donors On Aqds Reduction By Strain Pah-1mentioning

confidence: 94%

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Wang

et al. 2011

J Soils Sediments

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Purpose The anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) has great significance to PAHs' natural attenuation in contaminated sites. Previous studies mainly focused on anaerobic PAH degradation by mixed cultures with nitrate, sulfate, or Fe(III) oxides as electron acceptors, and the roles of pure cultures in the process was rarely reported. The aim of this paper is to isolate a pure culture that is capable of degrading phenanthrene anaerobically with anthraquinone-2,6-disulphonate (AQDS) as the sole electron acceptor and evaluate its environmental functions. Materials and methods A strain of pure culture was isolated from anodic solution of a microbial fuel cell via enrichment procedure with phenanthrene and AQDS under anaerobic conditions. Using the single colony isolation technique, a distinct colony was obtained and identified by the phenotypic and phylogenetic analysis. Its ability to reduce AQDS and degrade phenanthrene was conducted in serum bottles by standard anaerobic techniques (purged with 80% N 2 −20% CO 2 ). The concentration of AH 2 QDS and fructose was quantified by UV-vis spectrophotometer at 450 and 210 nm, respectively. Cells number was determined by direct plate counting on aerobic LB agar medium. The concentration of phenanthrene was determined using HPLC. Results and discussion Strain PAH-1 was identified as Pseudomonas aeruginosa. It could oxidize fructose or glucose to reduce AQDS and can support microbial growth by conserving energy from fructose to AQDS. It has the ability to degrade phenanthrene directly with AQDS as the sole electron acceptor (46.5% removal), and the microbial process may be AQDS dependent. The addition of small organic substances (fructose) could enhance the anaerobic biodegradation of phenanthrene from 46.5% to 56.7%. The anaerobic degradation of phenanthrene fits the pseudo-firstorder kinetics, giving the rate constants of 0.0233/day (R 2 =0.934) and 0.0328/day (R 2 =0.933) for non-fructose set and fructose set, respectively. Conclusions We successfully isolated a facultative anaerobe, P. aeruginosa strain PAH-1. This study was the first paper reporting that a pure culture (strain PAH-1) has the ability to anaerobically degrade phenanthrene with AQDS as the sole electron acceptor. The finding also explores the environmental significance of the Pseudomonas genus.

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“…For the reconstruction of phylogenetic trees almost full-length 16S rRNA gene sequences (>1400 bp) that were closely related to strain VDS11 T were retrieved from GenBank and validated using the EzTaxon-e Server (Kim et al, 2012). Strain VDS11 T showed highest sequence similarity with Corynebaterium humireducens MFC-5 T (98.7 %, 1401 bp) (Wu et al, 2011) and Corynebacterium nasicanis 2673/12 T (98.4 %, 1382 bp) (Baumgardt et al, 2015). The percentage pair wise sequence similarity of strain VDS11…”

mentioning

confidence: 99%

Corynebacterium pollutisoli sp. nov., isolated from hexachlorocyclohexane-contaminated soil

Negi

Singh

Schümann

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell

Cited by 80 publications

References 32 publications

Biogeochemical Reduction Processes in a Hyper-Alkaline Leachate Affected Soil Profile

Biogeochemical Reduction Processes in a Hyper-Alkaline Leachate Affected Soil Profile

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Corynebacterium pollutisoli sp. nov., isolated from hexachlorocyclohexane-contaminated soil

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