Corynebacterium Acnes and Other Anaerobic Diphtheroids From Human Skin

Marples, R. R.; McGinley, Kenneth J.

doi:10.1099/00222615-7-3-349

Cited by 95 publications

(53 citation statements)

References 17 publications

(14 reference statements)

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“…All the cultured bacteria were classical known members of the skin microbiota and the total c.f.u. cm À2 of the samples was of the same order as that reported previously (Evans, 1975;Marples & McGinley, 1974). The bacterial count was comparable to the results of molecular analysis, although it was not possible to describe the variation of the microbiota due to the limited number of isolates subjected to the identification procedures.…”

Section: Culture Analysissupporting

confidence: 71%

“…Previous culture-based studies have revealed that skin microbiota differ widely among different sites of the body (Marples & McGinley, 1974;Marples, 1982). To investigate the host-parasite relationship under physiologically steadystate conditions, we chose the forehead skin as the sampling site for five reasons.…”

Section: Sampling Sitementioning

confidence: 99%

“…2 3 10 6 Propionibacterium granulosum Molecular profiling of skin microbiota the genus level, the detected bacteria were classified into 24 genera, which indicates a much greater diversity than that of the formerly established resident bacteria, which consists of eight genera (Chiller et al, 2001). At the species level, the detected bacteria were classified into 19 species and 13 phylotypes, which included only 10 resident bacterial species formerly identified through culture methods (Tables 2 and 3) (Chiller et al, 2001;Marples & McGinley, 1974). All 19 species and 13 phylotypes were listed according to the total number of clones detected, as seen in Table 4.…”

Section: Bacterial Speciesmentioning

confidence: 99%

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Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling

Dekio

Hayashi

Sakamoto

et al. 2005

Journal of Medical Microbiology

131

116

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Although the micro-organisms forming the cutaneous microbiota are considered to play important roles in the modification and prevention of skin diseases, a comprehensive analysis of their composition has not yet been carried out because of difficulties in determining yet-to-be-cultured micro-organisms in the samples. Swab-scrubbed forehead skin samples of five healthy volunteers were analysed by profiling 16S rRNA genes, as well as by conventional culture methods, to provide a profile of the cutaneous microbiota that included yet-to-be-cultured bacteria from normal human skin. Cluster analyses of the 16S rRNA gene sequences indicated a marked increase in diversity compared with that derived from the culture methods. Nineteen previously recognized species and 13 novel phylotypes were obtained from the analysis of 416 clones. In addition to well-known bacteria such as Staphylococcus epidermidis and Propionibacterium acnes, phylotype A, the 16S rRNA gene of which is 97 % similar to that of Methylophilus methylotrophus, was detected in three of the five samples, in one of which it was the predominant clone. Culture-independent genetic profiling of 16S rRNA genes for detecting human cutaneous microbiota has allowed us to detect potentially novel components of the cutaneous microbiota in humans.

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Section: Culture Analysissupporting

confidence: 71%

Section: Sampling Sitementioning

confidence: 99%

Section: Bacterial Speciesmentioning

confidence: 99%

See 1 more Smart Citation

Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling

Dekio

Hayashi

Sakamoto

et al. 2005

Journal of Medical Microbiology

131

116

View full text Add to dashboard Cite

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“…A laboratory strain of Propionibacterium acnes (Type 1 of Marples & McGinley, 1974) and designated P37 was used exclusively in these investigations. The organism had previously been isolated from an acne blackhead lesion on a patient attending the Department of Dermatology outpatients clinic, Leeds General Infirmary.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Propionibacterium acnes with skin lipids in vitro

Gribbon

Cunliffe

Holland

1993

Journal of General Microbiology

135

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Propionibacterium acnes is the predominant microbial resident within the pilosebaceous follicles of sebum-rich areas of human skin. This study investigated the effects of known hydrophobic components of sebum on the physiology and nutrition of this microorganism, grown anaerobically at 33 "C, under defined conditions using continuous culture techniques. The medium used was chemically defined, comprising eight amino acids, with glucose as the main carbon energy source, and the culture pH was maintained at 5.6. The range of sebum lipids assayed was based on the C,, monounsaturated fatty acid 9-cis-octadecenoic acid (oleic acid). Stock micronized solutions were aseptically pulsed into continuous cultures in the presence and absence of glucose, and nutritional effects monitored. None of the lipid substrates significantly affected P. acnes growth either in terms of maximum specific growth rate (jzmm) or final culture biomass yield. Glycerol (3mgml-') was found to be a poor carbonlenergy source in comparison to glucose. Bacterial cells did, however, adhere with varying degrees, to the Merent lipid species, with maximum adherence occurring with the free fatty acid. This observation was confirmed by preliminary uptake experiments using ['4C]oleic acid. The interactive site for cell adherence may be the lipid-fibrillar layer associated with the cell surface of P. acnes, as discerned in electron microscopical studies. The findings of this investigation suggest that one function of the P. acnes lipase may be to aid colonization within the pilosebaceous follicle, by promoting cell adherence to components such as oleic acid.

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“…Propionibacterium acnes is the major bacterial inhabitant of the forehead and back of man (Marples & McGinley, 1974) and has been implicated in the disease acne vulgaris (Kirschbaum & Kligman, 1963). Propionibacterium acnes produces at least three exocellular enzymes: a lipase (Reisner et al, 1968), a protease (Marples & McGinley, 1974) and a hyaluronidase (Smith & Willet, 1968).…”

Section: Introductionmentioning

confidence: 99%