Corydalis edulis Maxim. Promotes Insulin Secretion via the Activation of Protein Kinase Cs (PKCs) in Mice and Pancreatic β Cells

Zheng, Jiao; Zhao, Yunfang; Lun, Qixing; Song, Yuelin; Shi, She‐Po; Gu, Xiao-Pan; Pan, Bo; Qu, Changhai; Li, Jun; Tu, Pengfei

doi:10.1038/srep40454

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…The HIT-T15 cells were a suitable clone for screening plant extracts for sulfonylurea-like activity via insulin secretion and sulfonylurea receptor 1 (SUR1)-binding studies [81]. In another study, HIT-T15 cells treated with whole plant extracts of Corydalis edulis displayed enhanced glucose-independent insulin secretion [136].…”

Section: Screening Plants and Natural Products For Antidiabetic Activitymentioning

confidence: 99%

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

2019

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Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence of diabetes, and the soaring cost of managing diabetes and its complications underscores an urgent need for safer, more efficient and affordable alternative treatments. Over 1200 plant species are reported in ethnomedicine for treating diabetes and these represents an important and promising source for the identification of novel antidiabetic compounds. Evaluating medicinal plants for desirable bioactivity goes hand-in-hand with methods in analytical biochemistry for separating and identifying lead compounds. This review aims to provide a comprehensive summary of current methods used in antidiabetic plant research to form a useful resource for researchers beginning in the field. The review summarises the current understanding of blood glucose regulation and the general mechanisms of action of current antidiabetic medications, and combines knowledge on common experimental approaches for screening plant extracts for antidiabetic activity and currently available analytical methods and technologies for the separation and identification of bioactive natural products. Common in vivo animal models, in vitro models, in silico methods and biochemical assays used for testing the antidiabetic effects of plants are discussed with a particular emphasis on in vitro methods such as cell-based bioassays for screening insulin secretagogues and insulinomimetics. Enzyme inhibition assays and molecular docking are also highlighted. The role of metabolomics, metabolite profiling, and dereplication of data for the high-throughput discovery of novel antidiabetic agents is reviewed. Finally, this review also summarises sample preparation techniques such as liquid–liquid extraction, solid phase extraction, and supercritical fluid extraction, and the critical function of nuclear magnetic resonance and high resolution liquid chromatography–mass spectrometry for the dereplication, putative identification and structure elucidation of natural compounds from evidence-based medicinal plants.

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Section: Screening Plants and Natural Products For Antidiabetic Activitymentioning

confidence: 99%

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

2019

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“…Glucagon-like peptide-1 (GLP-1), an endogenous glucoselowering hormone, also stimulates insulin secretion through the PLC/PKC-dependent pathway in isolated islets (40). The extract from Corydalis edulis Maxim., a widely grown plant in China, was found to enhance insulin secretion by the activation of PKC in pancreatic β cells (41). The results provide evidence for the protective role of PTF against PA-induced impairment of GSIS involving GPR40 signaling in INS-1 cells.…”

Section: Discussionmentioning

confidence: 62%

Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells

Feng

Duan

2017

International Journal of Molecular Medicine

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Prolonged elevated levels of free fatty acids (FFAs) contribute to the impairment of insulin secretion function of pancreatic β cells, a hallmark of type 2 diabetes, which is partly attributed to the dysfunction of G-protein-coupled receptor 40 (GPR40) signaling. Pollen Typhae total flavone (PTF), an extract from a Chinese herbal medicine named Pollen Typhae, has been reported to effectively treat type 2 diabetes, but the underlying mechanisms remain to be fully elucidated. In the present study, palmitic acid (PA), a saturated fatty acid, severely impaired glucose-stimulated insulin secretion (GSIS) in a time-dependent manner in INS-1 cells, and PTF treatment prevented the impairment in a dose-dependent manner. Moreover, PTF improved insulin secretion function in rats presenting with type 2 diabetes induced by a high-fat diet and low-dose streptozotocin. Furthermore, PA exposure for 24 h decreased the protein expression of GPR40, phospholipase C (PLC)β1, PLCβ3, and protein kinase C (PKC), and inhibited the activity of PLC and PKC stimulated by GW9508, a GPR40 agonist. In addition, PTF enhanced the protein expression of GPR40 and to a certain extent strengthened the protein expression of PKC, increased cellular levels of triphosphoinositide (IP3) and diacylglycerol (DAG), and promoted GW9508-stimulated activity of PLC and PKC reduced by PA in INS-1 cells, which were blocked by PLC inhibitor U-73122 and PKC inhibitor staurosporine, respectively. Additionally, the improvement in PA-induced impairment of GSIS by PTF in INS-1 cells was restrained by U-73122, staurosporine, and calcium channel inhibitor nifedipine, respectively. The results indicate that PTF exerts a protective role against PA-induced impairment of GSIS involving GPR40 signaling in INS-1 cells.

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“…HIT-T15 pancreatic islet cells were provided by Prof. Guan Y. F. (Peking University, Beijing, People’s Republic of China). The procedure for the assay of insulin secretion was the same as previously described, and each experiment was performed three times . Gliclazide (100 μM) was utilized as a positive control.…”

Section: Methodsmentioning

confidence: 99%

LC-MS-Guided Isolation of Insulin-Secretion-Promoting Monoterpenoid Carbazole Alkaloids from Murraya microphylla

Zheng

et al. 2018

J. Nat. Prod.

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Fifteen new structurally unique monoterpenoid carbazole alkaloids, including two pairs of epimers (1/2 and 3/4), three pairs of enantiomers (6a/6b, 7a/7b, and 8a/8b), and five optically pure analogues (5, 9−12), were obtained from a 95% aqueous EtOH extract of Murraya microphylla by a combination of bioassay-and LC-MS-guided fractionation procedures. Their structures were established based on NMR and HRESIMS data interpretation. The absolute configuration of compound 1 was determined via X-ray crystallographic data analysis and for all compounds by comparison of experimental and calculated ECD data. Compounds 1−5 were assigned as five new thujane−carbazole alkaloids, and compounds 6−12 as 10 new menthene−carbazole alkaloids linked through an ether or carbon−carbon bond. Compounds 1−12 promoted insulin secretion in the HIT-T15 cell line, 1.9−3.1-fold higher than the gliclazide control at 100 μM.

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Corydalis edulis Maxim. Promotes Insulin Secretion via the Activation of Protein Kinase Cs (PKCs) in Mice and Pancreatic β Cells

Cited by 10 publications

References 38 publications

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells

LC-MS-Guided Isolation of Insulin-Secretion-Promoting Monoterpenoid Carbazole Alkaloids from Murraya microphylla

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