Cortisol and glucocorticoid receptor 2 regulate acid secretion in medaka (Oryzias latipes) larvae

Lin, Cheng Huang; Hu, Huei-Jyun; Chuang, Hsin-Ju; Tsou, Yi-Ling; Hwang, Pung‐Pung

doi:10.1007/s00360-021-01390-w

Cited by 6 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Freshwater (FW, pH 7.0) and acidic freshwater (AFW, pH 4.0) prepared from local tap water were used to determine the effects of an acidic medium. In accordance with previous studies (Lin et al, 2015;Lin et al, 2021), the acidic medium was prepared by adding H 2 SO 4 to the FW; the concentrations of other ions in the AFW were same as those in the FW (see Supplemental Table S1). Fertilized zebrafish eggs were transferred to either FW or AFW and then incubated until sampling at 3 days postfertilization (dpf).…”

Section: Acclimation Experimentsmentioning

confidence: 99%

“…In zebrafish, HA and NHE3b in HRCs are responsible for approximately 70% and 30% of the apical proton secretion, respectively (Shih et al, 2008;Shih et al, 2012). However, NHE3 is the major route for apical proton secretion in acid-secreting ionocytes of medaka (Lin C. C. et al, 2012;Lin et al, 2021). The function of other acid secretion-related transporters in ionocytes is homologous in fishes (Hsu et al, 2014;Liu et al, 2016;Yan and Hwang, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Hormonal control plays an essential role in the maintenance of the acid-base balance in fishes (Guh et al, 2015;Yan and Hwang, 2019). Some hormones such as cortisol and endothelin-1 are induced after acid exposure in fishes (Guh et al, 2014;Lin et al, 2015;Lin et al, 2021). Overexpression and pharmacological approaches suggest that increased hormone levels can regulate the capacity for acid secretion by stimulating the mRNA expression and activity of acidsecreting transporters and/or ionocyte differentiation (Cruz et al, 2013;Guh et al, 2014;Lin et al, 2015;Lin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Vitamin D regulates transepithelial acid secretion in zebrafish (Danio rerio) larvae

et al. 2022

View full text Add to dashboard Cite

Maintenance of an acid-base balance is essential for normal physiological processes in vertebrates. Freshwater fishes live in an aquatic environment with variable pH, and their buffering capacity for acid-base balance in body fluids is weak. Thus, after acid exposure, fishes secrete excess acid to prevent internal acidosis. Acid-secreting ionocytes present in the adult gills and embryonic skin are primarily responsible for acid secretion, and H+-ATPase and Na+/H+ exchanger 3 (NHE3) are the two main transporters responsible for apical acid secretion. Vitamin D is a well-known hormone involved in the maintenance of Ca2+ homeostasis and is suggested to be involved in acid-base regulation by modulating the activity and/or mRNA expression of NHE3 in mammalian models. It remains unclear whether vitamin D is involved in acid secretion in fishes. The aim of the present study was to use zebrafish as a model to determine whether vitamin D and its receptors influence acid secretion. Our results indicated that the levels of 1α, 25-dihydroxyvitamin D3 (1α,25(OH)2D3), the bioactive vitamin D, were significantly increased in 3 days post-fertilization zebrafish larvae after exposure to acidic freshwater (AFW, pH 4.0). Exogenous 1α,25(OH)2D3 (20 μg/L) incubation substantially enhanced the mRNA expression of acid-secreting transporters and acid secretion at the skin of the entire body and each H+-ATPase-rich cell (HRC), a type of acid-secreting ionocyte. Furthermore, the expression of vitamin D receptors (VDRs) was identified in HRCs of zebrafish. When both VDRa and VDRb were knocked down, acid secretion and the mRNA expression of acid-secreting transporters were significantly decreased. Moreover, double knockdown of VDRa/b prevented the increase in acid secretion induced by AFW and 1α,25(OH)2D3 treatment. This study is the first to indicate that vitamin D is involved in acid secretion in fish.

show abstract

Section: Acclimation Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin D regulates transepithelial acid secretion in zebrafish (Danio rerio) larvae

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Saliva samples will be gathered using a Salivette collection system (hygienic saliva collection; Sarstedt) [ 36 ] and stored in a –20 °C freezer until they undergo analysis using the General Cortisol ELISA Kit (RK00639; ABclonal) [ 39 ]. The participants will be provided with specific instructions not to engage in eating, drinking, toothbrushing, or strenuous physical activity within the 30-minute interval preceding each saliva sampling session.…”

Section: Methodsmentioning

confidence: 99%

Effect of Baduanjin Qigong on Sleep Quality and Hyperarousal State in Adults With Chronic Insomnia: Protocol for a Randomized Controlled Trial

Xie,

et al. 2023

JMIR Res Protoc

View full text Add to dashboard Cite

Background Chronic insomnia (CI) is a mind-body disease that is commonly defined as a state of having disturbed daytime activities due to poor nighttime sleep quality. Baduanjin qigong (BDJQG) is widely used for CI in China. However, there is little scientific evidence to evaluate its effects on the hyperarousal state, which is closely associated with improved sleep quality. Objective The objective of the trial is to assess the therapeutic effects of BDJQG on sleep quality in patients with CI. Methods A randomized controlled trial will be conducted on 86 patients, who will be divided into a BDJQG group and a cognitive behavioral therapy for insomnia group at a ratio of 1:1. Interventions in both groups will be given to the participants 7 times a week for 8 weeks, and the participants will be followed up for 4 weeks. The primary outcome is the change in the Pittsburgh Sleep Quality Index from baseline to week 8. The secondary outcomes are the changes in the Hyperarousal Scale, Insomnia Severity Index, Fatigue Scale-14, wrist actigraphy, salivary cortisol level, and functional magnetic resonance imaging from baseline to week 8. All main analyses will be carried out on the basis of the intention-to-treat principle. Results This study was funded from January 2023. As of the submission of the manuscript, there were 86 participants. Data collection began in April 2023 and will end in January 2024. Data analysis is expected to begin in January 2024, with the publication of results expected in February 2024. Conclusions This study will present data concerning the clinical effects of BDJQG on CI. The results will help to demonstrate whether BDJQG is an effective therapy for improving sleep quality in association with a decreased hyperarousal level as a possible underlying mechanism. This study will provide much-needed knowledge for complementary and alternative therapy for patients with CI. Trial Registration China Clinical Registration Agency ChiCTR2300069241; https://chictr.org.cn/bin/project/ChiCTR2300069241 International Registered Report Identifier (IRRID) PRR1-10.2196/53501

show abstract

“…Previous studies have indicated that the mRNA expression of hormone metabolism genes and hormone levels are modulated in fish upon external changes in ion levels and pH values. Modulated hormone levels are vital for the maintenance of body fluid ions and acid-base homeostasis (Guh et al, 2015;Lin and Hwang, 2016;Yan and Hwang, 2019;Lin et al, 2021). 1a,25-DihydroxyvitaminD 3 (1a,25(OH) 2 D 3 ), a bioactive vitamin D, is a steroid hormone that regulates Ca 2+ uptake in vertebrates (Lips, 2006;Lin and Hwang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Vitamin D regulates ion regulation by affecting the ionocyte differentiation in zebrafish (Danio rerio) larvae

Lin

Chang²,

Liu³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Freshwater teleosts frequently face the stress of varied ion and pH levels; therefore, they have developed related defense mechanisms to maintain the homeostasis of body-fluid ion and acid-base balance. The different subtypes of ionocytes expressed in the branchial epithelium of adult fish or the skin of larvae are the major sites for fish ion regulation. 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3), the bioactive form of vitamin D, is a steroid hormone that is involved in the regulation of Ca2+ uptake and acid secretion in teleosts. Our results revealed that 1α,25(OH)2D3 levels were not changed in zebrafish larvae upon exposure to low Na+ freshwater compared to normal freshwater. In contrast, 1α,25(OH)2D3 levels were substantially higher in fish exposed to acidic and low Ca2+ freshwater than in those exposed to normal freshwater. Some hormones regulate ion regulation and acid secretion by modulating ionocyte differentiation and/or proliferation in teleosts; however, the role of vitamin D in this process is unclear. Zebrafish larvae were used as a model in the present study to explore the effect of vitamin D on ionocyte proliferation and/or differentiation. The present study indicated that 1α,25(OH)2D3 treatment increased the number of foxi3a-positive cells, ionocyte progenitors, and mature ionocytes. However, the number of P63-positive epidermal stem cells did not change in the zebrafish larvae treated with 1α,25(OH)2D3. These results revealed that vitamin D exerts a positive effect on the number of ionocytes by increasing the differentiation of ionocytes. Increased ionocyte differentiation by vitamin D is suggested to elevate the capacity of ion regulation and acid secretion in zebrafish to cope with external stress. The present findings indicate the role of vitamin D in the regulation of ionocyte differentiation and provide new insights into the mechanisms of stress adaptation of fish.

show abstract

Cortisol and glucocorticoid receptor 2 regulate acid secretion in medaka (Oryzias latipes) larvae

Cited by 6 publications

References 48 publications

Vitamin D regulates transepithelial acid secretion in zebrafish (Danio rerio) larvae

Vitamin D regulates transepithelial acid secretion in zebrafish (Danio rerio) larvae

Effect of Baduanjin Qigong on Sleep Quality and Hyperarousal State in Adults With Chronic Insomnia: Protocol for a Randomized Controlled Trial

Vitamin D regulates ion regulation by affecting the ionocyte differentiation in zebrafish (Danio rerio) larvae

Contact Info

Product

Resources

About