Corticotropin-releasing factor and neuropeptide Y mRNA levels are elevated in the preoptic area of socially subordinate rainbow trout

Doyon, Christian; Gilmour, Kathleen M.; Trudeau, Vance L.; Moon, Thomas W.

doi:10.1016/s0016-6480(03)00195-3

Cited by 137 publications

(89 citation statements)

References 60 publications

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“…These disparate npy responses suggest that Lep effects on npy expression may vary with nutritional status. In rainbow trout, npy expression is high in the telencephalon, preoptic area, and optic tectum, while relatively lower in the mediobasal hypothalamus (Doyon et al 2003). The Npy cell bodies in the diencephalon are mainly detected in the nucleus ventromedial thalamus and the ventral nucleus posterioris periventricularis , Pérez et al 2013, while Npy-containing fibers can be found throughout the brain, including the hypothalamus (Danger et al 1991).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of Npy, beta-endorphin, and adrenocorticotropic hormone has been demonstrated in the lateral tuberal hypothalamic region in various fish species (Meek & Nieuwenhuys 1998). In rainbow trout, npy expression is high in the telencephalon, preoptic area and optic tectum, and relatively lower in the mediobasal hypothalamus (Doyon et al 2003). While the Pomc genes (pomca1, pomca2, pomcb) are highly expressed in the rainbow trout hypothalamus (Leder & Silverstein 2006), and the Pomc cell bodies are found in the NLT region (Suzuki et al 1997, Tollemer et al 1999.…”

Section: Introductionmentioning

confidence: 99%

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Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Gong

Jönsson

Björnsson

2016

Journal of Molecular Endocrinology

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Leptin (Lep) is an anorexigenic hormone and regulates appetite-related neuropeptides in mammals. A number of neuropeptides have also been linked to appetite regulation in teleost fish, but Lep signaling activation and effects on appetite-regulating neurons are poorly elucidated in early vertebrates. This study uses cellular, tissue and organismal approaches to elucidate the acute, central Lep action in rainbow trout. The results demonstrate that Lep activates phosphorylation of protein kinase B (Akt) and signal transducer and activator of transcription 3 in rainbow trout hypothalamus-derived cells, and that the phosphatidylinositol-3-kinase (Pi3k) inhibitor LY294002 can suppress the Lep-induced Akt phosphorylation. Intracerebroventricular (ICV) Lep administration strongly suppresses food intake at the doses of 0.05 and 0.5 µg Lep fish −1 . At low dose, Lep stimulates hypothalamic transcription of anorexigenic cocaine-and amphetamineregulated transcript (Cart) and orexigenic neuropeptide Y. At high dose, Lep stimulates hypothalamic transcription of anorexigenic proopiomelanocortin (Pomc) A1, A2, and B, while coinjection with LY294002 reverses this upregulation. The data suggest that the anorexigenic action of Lep in rainbow trout is mediated through stimulation of the anorexigenic neuropeptides Pomc and Cart. Furthermore, ICV Lep treatment increases phosphor-Akt-immunoreactive cells in the nucleus lateralis tuberis, periventricular zone along infundibulum, and lateral recess surrounded by nucleus anterior tuberis, while LY294002 inhibits this effect. Lep receptor-immunoreactive cells are also predominant in these regions. These results demonstrate that Lep activates the Pi3k-Akt pathway in the lateral tuberal hypothalamus of rainbow trout for acute appetite regulation, indicating the conservation of anorexigenic Lep action in the mediobasal hypothalamus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Gong

Jönsson

Björnsson

2016

Journal of Molecular Endocrinology

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“…After acclimation, the divider was removed and the fish were allowed to interact for 8 or 24 h. During this time, social ranks were determined through indirect behavioral observations using mirrors placed at an angle above the tanks. Dominance was measured by assigning points for aggressive behavior, position in the tank, and food acquisition as per Doyon et al (2003) such that the fish within a pair with the higher score was identified as dominant and the fish with the lower score as subordinate. Fish were fed daily throughout the experiment.…”

Section: Experimental Designmentioning

confidence: 99%

“…As seen in the PVN of mammals (for review see Bakshi & Kalin 2000), exposure to a variety of stressors in teleosts can elicit an increase in the expression of CRF in the POA (Doyon et al 2003, Huising et al 2004. In the CNSS, while an increase in osmolality is associated with increase in both CRF and UI gene expression , physical restraint transiently activates CRF but not UI neurons (Lu et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Heads or tails? Stressor-specific expression of corticotropin-releasing factor and urotensin I in the preoptic area and caudal neurosecretory system of rainbow trout

Bernier¹,

Alderman²,

Bristow³

2007

Journal of Endocrinology

101

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Corticotropin-releasing factor (CRF)-and urotensin I (UI)-expressing cells of the preoptic area (POA) and caudal neurosecretory system (CNSS) are considered key contributors to the regulation of the stress response in fish; however, the expression pattern of these neurons to environmental and social challenges have not been compared in a single study. Therefore, we characterized in rainbow trout (Oncorhynchus mykiss) the central distribution of CRF and UI expression and quantified the POA and CNSS mRNA levels of both transcripts in response to hyperammonemia, hypoxia, isolation, or subordination. The tissue distribution demonstrated that the POA and the CNSS are dominant sites of CRF and UI expression. Comparison of the plasma cortisol levels in response to the diverse treatments showed that subordination was the most severe stressor followed by hyperammonemia, isolation, and hypoxia. In the POA, with the exception of subordination that had no effect on UI expression, all stressors resulted in increase in CRF and UI mRNA levels. In the CNSS, while hyperammonemia was associated with increase in CRF and UI mRNA levels, and hypoxia induced an increase in CRF expression, isolation caused a decrease in the expression of both transcripts, and subordination had no effect. Independent of the stressor, we found strong positive correlations between CRF and UI expression in the POA and the CNSS, and no correlation in the expression of either gene between regions. Overall, the results demonstrate that the contribution of POA and CNSS CRF and UI neurons to the stress response in rainbow trout is stressor-, time-, and region-specific.

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“…A third CRH receptor (CRH3) was found in the catfish, Ameiurus nebulosus (Arai et al 2001). However, because of the close structural and pharmacological similarity between the catfish CRH1 and CRH3 receptors, it was likely that the third receptor is the result of the extra genome duplication that occurs in a number of teleost lineages (Wolfe 2001, Mungpakdee et al 2008, Mulley et al 2009), including, for example, the white sucker (Morley et al 1991), goldfish and rainbow trout (Doyon et al 2003), where two Crh peptides are present. Interestingly, two CRH forms have been identified in the chondrichthyan, C. milii, although the identification of the CRH receptors has not been established (Nock et al 2011).…”

Section: Corticotrophinmentioning

confidence: 99%

MOLECULAR EVOLUTION OF GPCRS: CRH/CRH receptors

Lovejoy¹,

Chang²,

Lovejoy³

et al. 2014

Journal of Molecular Endocrinology

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Corticotrophin-releasing hormone (CRH) is the pivotal neuroendocrine peptide hormone associated with the regulation of the stress response in vertebrates. However, CRH-like peptides are also found in a number of invertebrate species. The origin of this peptide can be traced to a common ancestor of lineages leading to chordates and to arthropods, postulated to occur some 500 million years ago. Evidence indicates the presence of a single CRH-like receptor and a soluble binding protein system that acted to transduce and regulate the actions of the early CRH peptide. In vertebrates, genome duplications led to the divergence of CRH receptors into CRH1 and CRH2 forms in tandem with the development of four paralogous ligand lineages that included CRH; urotensin I/urocortin (Ucn), Ucn2 and Ucn3. In addition, taxon-specific genome duplications led to further local divergences in CRH ligands and receptors. Functionally, the CRH ligand-receptor system evolved initially as a molecular system to integrate early diuresis and nutrient acquisition. As multicellular organisms evolved into more complex forms, this ligand-receptor system became integrated with the organismal stress response to coordinate homoeostatic challenges with internal energy usage. In vertebrates, CRH and the CRH1 receptor became associated with the hypothalamo-pituitary-adrenal/interrenal axis and the initial stress response, whereas the CRH2 receptor was selected to play a greater role in diuresis, nutrient acquisition and the latter aspects of the stress response.

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Corticotropin-releasing factor and neuropeptide Y mRNA levels are elevated in the preoptic area of socially subordinate rainbow trout

Cited by 137 publications

References 60 publications

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway

Heads or tails? Stressor-specific expression of corticotropin-releasing factor and urotensin I in the preoptic area and caudal neurosecretory system of rainbow trout

MOLECULAR EVOLUTION OF GPCRS: CRH/CRH receptors

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