Corticosterone treatment inhibits cell proliferation in the gastric epithelium of suckling rats

Gama, Patrı́cia; Alvares, Eliana Parisi

doi:10.1007/pl00009964

Cited by 18 publications

(29 citation statements)

References 28 publications

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“…Previous observations also documented analogous changes in the stomach and colon [1, 2, 4, 5, 6, 7, 9, 10, 12, 14, 15, 28, 29]. It should be noted that a decrease in the values of all parameters is observed.…”

Section: Discussionmentioning

confidence: 71%

“…In well-oriented sections, the number of mitotic or labelled cells and the total number of cells were counted in the whole thickness of the mucosa in randomly selected fields [5, 7]. The slides were carefully moved so that the entire thickness of the gland was analyzed.…”

Section: Methodsmentioning

confidence: 99%

“…Glucocorticoid hormones play an important role in this regulation. There are much more than a hundred sources in the Medline database which have clearly proved that glucocorticoids have a strong oppressive influence on proliferative processes in the esophagus, stomach, different parts of the intestine and liver, and can lead to ulcer formation in the stomach and duodenum [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18]. At present, the situation with glucocorticoid action on proliferation in the digestive system is generally accepted.…”

Section: Introductionmentioning

confidence: 99%

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Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Гунин

Nikolaev²

2000

Digestion

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Background/Aims: It has been shown that acute or short-term treatments with glucocorticoids lead to a marked decrease in proliferation in the stomach and large intestine. The effects of more prolonged glucocorticoid treatment on cell renewal in these organs are not known. The present work was therefore undertaken to examine the proliferative activity in the stomach and colon during 2 months of glucocorticoid treatment in comparison with shorter treatments. Methods: Rats were treated with either the glucocorticoid triamcinolone acetonide or vehicle for 63, 33 or 3 days. Proliferation was assessed in the glandular epithelium of the fundal part of the stomach and in the epithelium of the colonic crypts using three criteria: the mitotic index; the bromodeoxyuridinelabelling index, and the proliferating cell nuclear antigen-labelling index (percentage of mitotic or labelled cells). Results: Treatment with glucocorticoid for 63 days resulted in a very significant increase in all proliferative parameters tested in the gastric mucosa and the colonic crypts. On the contrary, treatments with glucocorticoid for 3 or 33 days had a marked inhibitory influence on proliferation in these tissues. Conclusion: As opposed to treatments for 3 or 33 days, glucocorticoid treatment for 2 months leads to an increase in the number of cycling cells in the gastric and colonic mucosae.

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Section: Discussionmentioning

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Гунин

Nikolaev²

2000

Digestion

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“…These maturation changes have been correlated with enhanced glucocorticoid levels (26) and increased epithelial cell migration rate (26). We have shown that plasma corticosteroid levels are highly elevated in food-deprived rats aged 18 and 22 days (27). However, administration of corticosterone inhibited epithelial proliferation of the stomach of fed suckling rats (27) but no effect could be demonstrated in the small intestine (28).…”

Section: Discussionmentioning

confidence: 93%

“…We have shown that plasma corticosteroid levels are highly elevated in food-deprived rats aged 18 and 22 days (27). However, administration of corticosterone inhibited epithelial proliferation of the stomach of fed suckling rats (27) but no effect could be demonstrated in the small intestine (28). Higher levels of corticosterone were also obtained for suckling milkdeprived rats receiving prostaglandin E 2 (PDGE 2 ) (29).…”

Section: Discussionmentioning

confidence: 99%

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Palanch

Alvares

1998

Braz J Med Biol Res

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Food deprivation has been found to stimulate cell proliferation in the gastric mucosa of suckling rats, whereas the weanling period has been reported to be unresponsive in terms of proliferative activity. In the present study we analyze regional differences in the effect of milk or food deprivation on cell proliferation of the epithelia of the esophagus and of five segments of small intestine in suckling, weanling and newly weaned Wistar rats of both sexes. DNA synthesis was determined using tritiated thymidine to obtain labeling indices (LI); crypt depth and villus height were also determined. Milk deprivation decreased LI by 50% in the esophagus (from 15 to 8.35%) and small intestine (from 40 to 20%) of 14-day-old rats. In 18-day-old rats, milk and food deprivation decreased LI in the esophagus (from 13 to 5%) and in the distal segments of the small intestine (from 36-40 to 24-32%). In contrast, the LI of the epithelia of the esophagus (5%) and of all small intestine segments (around 30%) of 22-day-old rats were not modified by food deprivation. Crypt depth did not change after treatment (80 to 120 µm in 14-and 22-day-old rats, respectively). Villus height decreased in some small intestine segments of unfed 14-(from 400 to 300 µm) and 18-day-old rats (from 480 to 360 µm). The results show that, contrary to the stomach response, milk deprivation inhibited cell proliferation in the esophagus and small intestine of suckling rats, demonstrating the regional variability of each segment of the gastrointestinal tract in suckling rats. In newly weaned rats, food deprivation did not alter the proliferation of these epithelia, similarly to the stomach, indicating that weanling is a period marked by the insensitivity of gastrointestinal epithelia to dietary alterations.

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Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments

et al. 2000

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Glucocorticoids take part in the intense morphofunctional modifications that occur in the gastric mucosa during fetal and postnatal development. Two studies were designed to evaluate corticoids role in gastric cell proliferation and apoptosis in developing rats: in vivo, using suckling animals; in vitro, using gastric explants obtained from 20‐day fetuses. These explants were cultured in DMEM/F12 medium treated or not with 50 ng/ml of corticosterone; after 22 hr, vincristine was added to the medium for 2 hr to block mitosis. The metaphasic index decreased significantly after the 24‐hr treatment (controls: 1.52 ± 0.53; treated: 0.40 ± 0.21) and apoptotic cells were visualized under light and electron microscopy. Fifteen‐day‐old rats were treated with hydrocortisone (25 mg/Kg) for 3 days, and injected with BrDU (100 mg/Kg) 1 hr before sacrifice on the 18th day. BrDu‐labeled and non‐labeled cells were counted to determine the labeling index of epithelial cells. As apoptotic cells are rapidly eliminated, other animals were treated for only 2–3 hr. Sections were investigated for the presence of apoptotic cells, using morphological criteria and TUNEL labeling. Hydrocortisone significantly reduced the labeling index (controls: 15.6 ± 1.6 vs. treated: 11.7 ± 1.1), besides altering the body weight gain. Hydrocortisone treatment doubled the number of apoptotic cells after 2 hr, and quadruplicated it after 3 hr. The results demonstrated that glucocorticoids inhibit cell proliferation in the gastric epithelium of fetuses and suckling rats and increase apoptotic rates, suggesting the exit from cell cycle. Anat Rec 260:213–221, 2000. © 2000 Wiley‐Liss, Inc.

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Corticosterone treatment inhibits cell proliferation in the gastric epithelium of suckling rats

Cited by 18 publications

References 28 publications

Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments

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