Current nonhuman models for bronchopulmonary dysplasia have not included perinatal infection. We studied the effects of antenatal Ureaplasma urealyticum (Uu) infection in the 125-d immature baboon. Ten 125-d gestation (term ϭ 185 d) baboon dams were delivered after intra-amniotic inoculation with Uu. Serial blood and tracheal aspirate samples were analyzed for Uu colony-forming units, IL-6, IL-8, and cell counts. Physiologic parameters were serially recorded. Lung histology was examined after 14 d of ventilation and compared with unexposed controls. All Uu-exposed animals had Ͼ4 ϫ 10 2 CFU in tracheal aspirate at 24 h. Four of nine Uu animals remained heavily colonized [(ϩ) Uu] at necropsy (Ͼ6 ϫ 10 3 ). Five animals had negative or low tracheal colony-forming units. All Uu animals had significant increases for white blood cells, IL-6, and IL-8 in amniotic and fetal lung fluid. Compared with controls, (ϩ) Uu animals had significantly higher fraction of inspired oxygen, airway pressures, oxygenation index, and ventilation efficiency index between 48 and 240 h and had significantly elevated tracheal IL-6 and IL-8 concentrations between 72 and 240 h. Compared with controls (Ϫ) Uu animals had significantly better oxygenation index and ventilation efficiency index scores between 48 and 144 h. Lung histopathology in both Uu groups showed more severe bronchiolitis and interstitial pneumonitis compared with controls. Two patterns of disease were observed after Uu perinatal infection. Persistent colonization manifested a picture consistent with acute pneumonitis, worse lung function from 2 to 10 d, and prolonged elevated tracheal cytokines. Colonized animals that subsequently cleared Uu from the lung demonstrated early improved lung function compared with unexposed controls yet still manifested mixed bronchiolitis and interstitial pneumonitis at necropsy. Inherent immune system responses may determine outcome of perinatal Ureaplasma colonization. The pathogenesis of bronchopulmonary dysplasia (BPD) is incompletely understood, but pulmonary inflammation seems to play a critical key role (1-3). Pulmonary inflammation may be initiated by various insults, including hyperoxia, volutrauma, and infection. Recent reports suggest an important role for both postnatal and perinatal infection in the pathogenesis of BPD (4 -7).Evidence for microbial invasion of the amniotic cavity has been found in up to 80% of preterm births (8
ABSTRACT
797(Uu)(9 -11). The presence of Uu in the preterm respiratory tract has been correlated with elevated cellular and molecular markers of inflammation and associated with an increased risk for BPD (7,(12)(13)(14). Current nonhuman models for BPD are limited by the lack of perinatal infection as a confounding factor. A previous pilot study from this center demonstrated worse clinical and histologic hyaline membrane disease after Uu colonization of the 140-d preterm baboon (15). However, Ureaplasma colonization was conferred postnatally, and all animals were exposed to hyperoxia [fraction of inspired oxy...