Corticosteroid therapy in avian muscular dystrophy: Evaluation by magnetic resonance relaxation times

Misra, Lalith K.; Entrikin, Richard K.

doi:10.1016/0014-4886(88)90096-9

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…Acceleration of the rate of fusion of quiescent myoblasts, so-called satellite cells (Bischoff, 1990), with DMD muscle fibers would enhance muscle regeneration. This hypothesis is supported by numerous reports (Askanas et al, 1986;Braun et al, 1989;Guerriero and Florini, 1980;Kaplan et al, 1990;Misra and Entrikin, 1988;Brown, 1991), although Hardiman et al (1992) found that PDN inhibited myogenesis in muscle cultures in six of eight Duchenne/Becker patients. This result is, however, tentatively explained by the authors, who among other hypotheses, suggest that the stimulating effect of PDN takes place at an early age.…”

Section: Discussionmentioning

confidence: 64%

“…The effect of glucocorticoids on the differentiation of many cell types and on the regulation of gene expression is well documented (Burnstein and Cidlowski, 1989). We and others (Askanas et al, 1986;Braun et al, 1989;Guerriero and Florini, 1980;Kaplan et al, 1990;Misra and Entrikin, 1988;Sklar and Brown;199 1) have described the stimulating effects of glucocorticoids on the in vitro differentiation of skeletal muscle cells. It was therefore of interest to study the effect of PDN in mdx mice.…”

Section: Introductionmentioning

confidence: 91%

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Prednisolone enhances myogenesis and dystrophin‐related protein in skeletal muscle cell cultures from mdx mouse

Passaquin¹,

Metzinger²,

Leger

et al. 1993

J of Neuroscience Research

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The differentiation of skeletal muscle cells from mdx mice which lack dystrophin expression was examined after glucocorticoid treatment, namely alpha-methylprednisolone (PDN). Primary skeletal muscle cell cultures were established from newborn mdx, congenic C57BL/10, and allogenic BALB/C mice. We show that PDN promotes the myogenesis of both mdx- and control mice-derived cultures as determined by 1) the number of myotubes, 2) acetylcholine receptors, and 3) dystrophin and dystrophin-related protein levels. These results support the hypothesis that PDN could enhance the myogenesis of satellite cells and increase dystrophin-related protein expression in DMD treated patients.

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Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 91%

Prednisolone enhances myogenesis and dystrophin‐related protein in skeletal muscle cell cultures from mdx mouse

Passaquin¹,

Metzinger²,

Leger

et al. 1993

J of Neuroscience Research

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“…In mdx mice, however, Weller et al (1991) found that PDN did not change the in vivo prevalence of fibre necrosis and regeneration. (2) A direct effect on myogenesis has been noted (Guerriero & Florini, 1980;Askanas et al, 1986;Misra & Entrikin, 1988;Braun et al, 1989;Kaplan et al, 1990;Sklar & Brown, 1991;Passaquin et al, 1993;; an acceleration of the rate of fusion of quiescent myoblasts, so-called satellite cells (Bischoff, 1990), with DMD muscle fibres would therefore enhance muscle regeneration. (3) Protection of the newly formed myotubes by antioxidant action and/or inhibition of myotube apoptosis (Sklar & Brown, 1991).…”

Section: Discussionmentioning

confidence: 99%

Modulation by prednisolone of calcium handling in skeletal muscle cells

Metzinger

Passaquin

Leijendekker

et al. 1995

British J Pharmacology

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1 Increased calcium (Ca2") influx has been incriminated as a potential pathological mechanism in the chronic skeletal muscle degeneration exhibited by Duchenne muscular dystrophy (DMD) patients. We have studied the influence of the glucocorticoid a-methylprednisolone (PDN), the only drug known to have a beneficial effect on the degenerative course of DMD, on Ca2" handling in the C2 skeletal muscle cell line. 2 PDN, when added 3 days (when myoblasts start to fuse into myotubes) after cell seeding, led to a 2 to 4 fold decrease in cellular Ca21 uptake. This decrease was independent of the extracellular Ca2+ concentration applied to cells. The effect took at least 24 h in order to become established (PDN of l0-5 M) and took longer for lower PDN concentrations (ECm of ca. 10-6 M at day 5, 10-6.5 M at day 7 and i0-7 5 M at day 9 in culture). 3 Cellular calcium accumulation was also decreased in PDN-treated myotubes exposed to 45Ca2+-containing medium for 1 to 6 days. 4 No effect of PDN was seen on 45Ca2+ efflux; a decrease in the amount of 45Ca2+released was observed due to the reduction of cellular 45Ca2+ loading.

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“…Although MRI distinguishes from CT as high resolution and radiation free technique (1,10,18,19,20), the MR studies on avian are mostly focused on the clinical cases (8,14,21,26). Some of the researches has been carried out to calculate the different tissue densities such as fat or water ratios in the body by using MR techniques (9,29).…”

Section: Introductionmentioning

confidence: 99%

High resolution 3D magnetic resonance imaging of the visceral organs in chicken (Gallus domesticus) by 3 Tesla MR unit and 15-channel transmit coil

OTO¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Imaging studies conducted on the modern imaging techniques for birds are limited and probably insufficient for the clinicians. As in mammals, magnetic resonance (MR) imaging (MRI) can be used as a convenient method for the diagnosis and treatment of the avian diseases. In this study, the whole bodies of 2 male and 2 female chickens were imaged by a 3 Tesla superconductive magnet and 15-channel transmit-receive birdcage coil. After acquisition of three dimensional (3D) T1, T2 and proton density weighted (W) MR images; bodies were frozen in same position with the one in imaging process and sliced from matching sections with original and reformatted MR images. Anatomic structures were identified and labeled in both MR images and cadaver sections. After that, 3D multiplanar reconstruction was performed on the MR images. On T1W images, it was observed that the anatomical details were superior due to the high geometric resolution. On T2W images, the tissue contrast differences and fluid filled ducts were clearly detected. On three orthogonal and oblique planes reformatted and maximum intensity projection (MIP) colored images, the anatomic details were more clearly determined and the tissues were more easily distinguished from each other with high geometric and contrast resolution. The aim of this study was to define MRI features of the tissues, and to provide an overview of MRI anatomy of the avian body structures. Besides, the most convenient sequences for the avian MRI were also designated.

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Corticosteroid therapy in avian muscular dystrophy: Evaluation by magnetic resonance relaxation times

Cited by 5 publications

References 21 publications

Prednisolone enhances myogenesis and dystrophin‐related protein in skeletal muscle cell cultures from mdx mouse

Prednisolone enhances myogenesis and dystrophin‐related protein in skeletal muscle cell cultures from mdx mouse

Modulation by prednisolone of calcium handling in skeletal muscle cells

High resolution 3D magnetic resonance imaging of the visceral organs in chicken (Gallus domesticus) by 3 Tesla MR unit and 15-channel transmit coil

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