Corticospinal Tract Tracing in the Marmoset with a Clinical Whole-Body 3T Scanner Using Manganese-Enhanced MRI

Demain, Boris; Davoust, Carole; Plas, Benjamin; Bolan, Faye; Boulanouar, Kader; Renaud, Luc; Darmana, R.; Vaysse, Laurence; Vieu, Christophe; Loubinoux, Isabelle

doi:10.1371/journal.pone.0138308

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…Since the globus pallidus was only directly injured in two animals, remote lenticular fasciculus injury in the other animals likely reflects degeneration of cortico-striatalthalamo-cortical loop axons. Our hypothesis is supported by retrograde tracing studies in marmosets showing that the primary motor cortex receives thalamic input from ventral anterior and ventrolateral nuclei [44], [45] and is consistent with the observed putamen atrophy, as neurons in primary motor cortex project to the putamen [46]. The lesions we observed could also be attributed to damage of other corticopontine fibres such as the reticulospinal tract, known to be involved in hand movement and reaching in humans and macaques [47].…”

Section: Discussionsupporting

confidence: 89%

A Reproducible New Model of Focal Ischemic Injury in the Marmoset Monkey: MRI and Behavioural Follow-Up

Friec

Desmoulin

Demain

et al. 2020

Transl. Stroke Res.

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Section: Discussionsupporting

confidence: 89%

A Reproducible New Model of Focal Ischemic Injury in the Marmoset Monkey: MRI and Behavioural Follow-Up

Friec

Desmoulin

Demain

et al. 2020

Transl. Stroke Res.

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“…This test determines the dominant paw of the rat. This test was validated in previous studies by the group (Demain et al, 2015;Davoust et al, 2017;Cirillo et al, 2018). All rats underwent the strength test (3 trials/paw/day) on 3 consecutive days, and a mean score was calculated for each week, before and after the injury.…”

Section: Grip Strength Testmentioning

confidence: 99%

Long-Term Intranasal Nerve Growth Factor Treatment Favors Neuron Formation in de novo Brain Tissue

Colitti

Desmoulin

Friec

et al. 2022

Front. Cell. Neurosci.

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ObjectiveTo date, no safe and effective pharmacological treatment has been clinically validated for improving post-stroke neurogenesis. Growth factors are good candidates but low safety has limited their application in the clinic. An additional restraint is the delivery route. Intranasal delivery presents many advantages.Materials and MethodsA brain lesion was induced in twenty-four rats. Nerve growth factor (NGF) 5 μg/kg/day or vehicle was given intranasally from day 10 post-lesion for two periods of five weeks, separated by a two-week wash out period with no treatment. Lesion volume and atrophy were identified by magnetic resonance imaging (MRI). Anxiety and sensorimotor recovery were measured by behavior tests. Neurogenesis, angiogenesis and inflammation were evaluated by histology at 12 weeks.ResultsRemarkable neurogenesis occurred and was visible at the second and third months after the insult. Tissue reconstruction was clearly detected by T2 weighted MRI at 8 and 12 weeks post-lesion and confirmed by histology. In the new tissue (8.1% of the lesion in the NGF group vs. 2.4%, in the control group at 12 weeks), NGF significantly increased the percentage of mature neurons (19% vs. 7%). Angiogenesis and inflammation were not different in the two groups. Sensorimotor recovery was neither improved nor hampered by NGF during the first period of treatment, but NGF treatment limited motor recovery in the second period.InterpretationThe first five-week period of treatment was very well tolerated. This study is the first presenting the effects of a long treatment with NGF and has shown an important tissue regeneration rate at 8 and 12 weeks post-injury. NGF may have increased neuronal differentiation and survival and favored neurogenesis and neuron survival through subventricular zone (SVZ) neurogenesis or reprogramming of reactive astrocytes. For the first time, we evidenced a MRI biomarker of neurogenesis and tissue reconstruction with T2 and diffusion weighted imaging.

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“… 31 As such, many experiments are still possible without the use of geometrically optimized or contoured coils, with standard surface/volume coils often an excellent option for presurgical mapping, diagnostics, and ex vivo sequences. Across all MRI systems, systemic administration (or even local injections) of contrast agents such as manganese can be used to boost the contrast of specific proton relaxivities, 60 , 61 thus improving the acquired MR signal.…”

Section: Marmoset Imaging Across Mri Platformsmentioning

confidence: 99%

Magnetic Resonance Imaging of Marmoset Monkeys

et al. 2020

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The use of the common marmoset monkey (Callithrix jacchus) for neuroscientific research has grown markedly in the last decade. Magnetic resonance imaging (MRI) has played a significant role in establishing the extent of comparability of marmoset brain architecture with the human brain and brains of other preclinical species (eg, macaques and rodents). As a non-invasive technique, MRI allows for the flexible acquisition of the same sequences across different species in vivo, including imaging of whole-brain functional topologies not possible with more invasive techniques. Being one of the smallest New World primates, the marmoset may be an ideal nonhuman primate species to study with MRI. As primates, marmosets have an elaborated frontal cortex with features analogous to the human brain, while also having a small enough body size to fit into powerful small-bore MRI systems typically employed for rodent imaging; these systems offer superior signal strength and resolution. Further, marmosets have a rich behavioral repertoire uniquely paired with a lissencephalic cortex (like rodents). This smooth cortical surface lends itself well to MRI and also other invasive methodologies. With the advent of transgenic modification techniques, marmosets have gained significant traction as a powerful complement to canonical mammalian modelling species. Marmosets are poised to make major contributions to preclinical investigations of the pathophysiology of human brain disorders as well as more basic mechanistic explorations of the brain. The goal of this article is to provide an overview of the practical aspects of implementing MRI and fMRI in marmosets (both under anesthesia and fully awake) and discuss the development of resources recently made available for marmoset imaging.

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Corticospinal Tract Tracing in the Marmoset with a Clinical Whole-Body 3T Scanner Using Manganese-Enhanced MRI

Cited by 6 publications

References 41 publications

A Reproducible New Model of Focal Ischemic Injury in the Marmoset Monkey: MRI and Behavioural Follow-Up

A Reproducible New Model of Focal Ischemic Injury in the Marmoset Monkey: MRI and Behavioural Follow-Up

Long-Term Intranasal Nerve Growth Factor Treatment Favors Neuron Formation in de novo Brain Tissue

Magnetic Resonance Imaging of Marmoset Monkeys

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