Cortical neurogenesis and morphogens: diversity of cues, sources and functions

Tiberi, Luca; Vanderhaeghen, Pierre; Ameele, Jelle van den

doi:10.1016/j.ceb.2012.01.010

Cited by 93 publications

(73 citation statements)

References 81 publications

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“…Taking advantage of this attribute, we examined the mechanism of process formation in neurally induced mouse ESCs and found that the extension of cellular processes is dependent on the extrinsic apico-basal epithelial polarity of the host tissue, as well as on the intrinsic differentiation state of the grafted cells. The role of intrinsic and extrinsic factors in determining the behavior of neural progenitors has attracted researchers' interest [40]. As described in the ''Introduction'' section, the entire neurogenic process, from an undifferentiated stem cell to a terminally differentiated cortical neuron, can be tracked within the defined conditions of a culture dish [1,3,4], indicating that intrinsic pathways are sufficient for the overall differentiation of cortical neurons.…”

Section: Discussionmentioning

confidence: 99%

Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells

Nagashima

Suzuki

Shitamukai

et al. 2014

Stem Cells and Development

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Current stem cell technologies have enabled the induction of cortical progenitors and neurons from embryonic stem cells (ESCs) and induced pluripotent stem cells in vitro. To understand the mechanisms underlying the acquisition of apico-basal polarity and the formation of processes associated with the stemness of cortical cells generated in monolayer culture, here, we developed a novel in utero transplantation system based on the moderate dissociation of adherens junctions in neuroepithelial tissue. This method enables (1) the incorporation of remarkably higher numbers of grafted cells and (2) quantitative morphological analyses at single-cell resolution, including time-lapse recording analyses. We then grafted cortical progenitors induced from mouse ESCs into the developing brain. Importantly, we revealed that the mode of process extension depends on the extrinsic apicobasal polarity of the host epithelial tissue, as well as on the intrinsic differentiation state of the grafted cells. Further, we successfully transplanted cortical progenitors induced from human ESCs, showing that our strategy enables investigation of the neurogenesis of human neural progenitors within the developing mouse cortex. Specifically, human cortical cells exhibit multiple features of radial migration. The robust transplantation method established here could be utilized both to uncover the missing gap between neurogenesis from ESCs and the tissue environment and as an in vivo model of normal and pathological human corticogenesis.

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Section: Discussionmentioning

confidence: 99%

Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells

Nagashima

Suzuki

Shitamukai

et al. 2014

Stem Cells and Development

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“…Upon closer inspection, however, it was also clear that this was accompanied by a suppression of neurogenesis, in a manner reminiscent of the effect of activating Notch in this region (Gaiano et al, 2000;reviewed in Imayoshi et al, 2013;Tiberi et al, 2012;Pierfelice et al, 2011;Cau and Blader, 2009), which is discussed in detail below. Importantly, these studies highlighted that CNTN1 itself, often assumed to be a post-mitotic marker, in fact is normally expressed in proliferating cells of the cortical ventricular zone, though the precise identity and state of these cells has not been determined.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The role of Gpi-anchored axonal glycoproteins in neural development and neurological disorders

Gennarini

Bizzoca

Picocci

et al. 2017

Molecular and Cellular Neuroscience

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“…There were 123 genes in Cluster 2, including transcription factors correlated with neuron differentiation and axon guidance molecules. Gene members of the Notch, Fgf, Shh and Wnt signaling pathways, all of which are important for cortical development (Gaiano et al, 2000;Cornell and Eisen, 2005;Louvi and Artavanis-Tsakonas, 2006;Dong et al, 2012;Tiberi et al, 2012), were also listed in Cluster 2. Cluster 3 included 100 genes, those of which regulate cell motion, neuron migration and cell localization (Table 3 and 4).…”

Section: Genome-wide Transcriptome Analysis Revealed That Nfib Regulamentioning

confidence: 99%

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

Betancourt

Katzman

Chen

2013

J of Comparative Neurology

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During development of the cerebral cortex, neural stem cells divide to expand the progenitor pool and generate basal progenitors, outer radial glia and cortical neurons. As these newly born neurons differentiate, they must properly migrate toward their final destination in the cortical plate, project axons to appropriate targets, and develop dendrites. However, a complete understanding of the precise genetic mechanisms regulating these steps is lacking. Here we show that a member of the nuclear factor one (NFI) family of transcription factors, NFIB, is essential for many of these processes in mice. We performed a detailed analysis of NFIB expression during cortical development, and investigated defects in cortical neurogenesis, neuronal migration and differentiation in NfiB −/− brains. We found that NFIB is strongly expressed in radial glia and corticofugal neurons throughout cortical development. However, in NfiB −/− cortices, radial glia failed to generate outer radial glia, subsequently resulting in a loss of late basal progenitors. In addition, corticofugal neurons showed a severe loss of axonal projections, while late-born cortical neurons displayed defects in migration and ectopically expressed the early-born neuronal marker, CTIP2. Furthermore, gene expression analysis, by RNA-sequencing, revealed a misexpression of genes that regulate the cell cycle, neuronal differentiation and migration in NfiB −/− brains. Together these results demonstrate the critical functions of NFIB in regulating cortical development.

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Cortical neurogenesis and morphogens: diversity of cues, sources and functions

Cited by 93 publications

References 81 publications

Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells

Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells

The role of Gpi-anchored axonal glycoproteins in neural development and neurological disorders

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

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