Cortical Hemodynamic Responses Under Focused Ultrasound Stimulation Using Real-Time Laser Speckle Contrast Imaging

Yuan, Yi; Zhao, Yanchao; Jia, Haikun; Li, Mengyang; Hu, Shuo; Li, Yingwei; Li, Xiaoli

doi:10.3389/fnins.2018.00269

Cited by 20 publications

(16 citation statements)

References 34 publications

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“…Meanwhile, the differentiation of motor responses throughout the step-wise scanning may also indicate a brain-region specific effect by tFUS. Besides the aforementioned studies evaluating the ultrasound evoked motor-related responses, very recently, 2.9 MHz focused ultrasound (FUS) was shown to also alter the local cerebral blood flow (CBF), monitored through laser speckle contrast imaging [41]. This cortical hemodynamic alteration by FUS provides further evidence in support of targeted activation through ultrasound.…”

Section: Tfus Directly Activates Local Brain Tissuementioning

confidence: 99%

“…Here we present three potential interpretations for how the brain responds to ultrasound energy deposition (Figure 1). Given the extensive electrophysiological [20,21,30,32], neurovascular [24,29,34,41], motor [14,16–18,22,23] and cognitive [32,35,36] evidences in support of tFUS-induced direct neural effects, we further examine whether the auditory pathway in the small brain volumes of rodent or mouse models dictates or impacts the observed activation patterns.…”

Section: Introductionmentioning

confidence: 99%

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On the neuromodulatory pathways of the in vivo brain by means of transcranial focused ultrasound

Niu

2018

Current Opinion in Biomedical Engineering

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For last decade, low-intensity transcranial focused ultrasound (tFUS) has been rapidly developed for a myriad of successful applications in neuromodulation. tFUS possesses high spatial resolution, focality and depth penetration as a noninvasive neuromodulation tool. Despite the promise, confounding activation can be observed in rodents when stimulation parameters are not selected carefully. Here we summarize the existing classes of observations for ultrasound neuromodulation: ultrasound directly activates a localized area, or ultrasound indirectly activates auditory pathways, which further propagates to other cortical networks. We also present control in vivo animal studies, which suggest that underlying tFUS brain modulation is characterized by localized activation independent of auditory networks activations.

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Section: Tfus Directly Activates Local Brain Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

On the neuromodulatory pathways of the in vivo brain by means of transcranial focused ultrasound

Niu

2018

Current Opinion in Biomedical Engineering

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“…The LIPUS system was similar to that used in our previous paper (Yuan et al, 2018). In the LIPUS group, the fundamental frequency (FF), pulsed repetition frequency (PRF), duty cycle (DC), and stimulation duration (SD) of the pulsed ultrasound were 500 kHz, 500 Hz, 50% and 30 s, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies used LIPUS to stimulate different regions of the brain of rodent animals, monkeys, and humans. They found that LIPUS can modulate neuronal activity (Tyler et al, 2008; Tufail et al, 2010), neural network connections (Yu et al, 2016), cerebral hemodynamics (Yang et al, 2018; Yuan et al, 2018), and water molecular diffusion (Yuan et al, 2017). Previous studies have also shown that LIPUS can cause a reduction in the occurrence of epileptic EEG bursts and severity of epileptic behavior and can also result in fewer spontaneous recurrent seizures and improved performance in behavioral tasks assessing sociability and depression in the chronic period of epilepsy (Min et al, 2011; Hakimova et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Stimulation Modulates the Nonlinear Dynamics of Local Field Potentials in Temporal Lobe Epilepsy

Yang

Yan

et al. 2019

Front. Neurosci.

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Low-intensity pulsed ultrasound stimulation (LIPUS) can inhibit seizures associated with temporal lobe epilepsy (TLE), which is the most common epileptic syndrome in adults and accounts for more than half of the cases of intractable epilepsy. Electroencephalography (EEG) signal analysis is an important method for studying epilepsy. The nonlinear dynamics of epileptic EEG signals can be used as biomarkers for the prediction and diagnosis of epilepsy. However, how ultrasound modulates the nonlinear dynamic characteristics of EEG signals in TLE is still unclear. Here, we used low-intensity pulsed ultrasound to stimulate the CA3 region of kainite (KA)-induced TLE mice, simultaneously recorded local field potentials (LFP) in the stimulation regions before, during, and after LIPUS. The nonlinear characteristics, including complexity, approximate entropy of different frequency bands, and Lyapunov exponent of the LFP, were calculated. Compared with the control group, the experimental group showed that LIPUS inhibited TLE seizure and the complexity, approximate entropy of the delta (0.5–4 Hz) and theta (4–8 Hz) frequency bands, and Lyapunov exponent of the LFP significantly increased in response to ultrasound stimulation. The values before ultrasound stimulation were higher ∼1.87 (complexity), ∼1.39 (approximate entropy of delta frequency bands), ∼1.13 (approximate entropy of theta frequency bands) and ∼1.46 times (Lyapunov exponent) than that after ultrasound stimulation ( p < 0.05). The above results demonstrated that LIPUS can alter nonlinear dynamic characteristics and provide a basis for the application of ultrasound stimulation in the treatment of epilepsy.

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“…Cerebral hemodynamics, in particular, attracts tremendous interests as it is spatially and temporally regulated with neural activity through neurovascular coupling. The previous studies of optical neural activity imaging mainly use the optical methods such as laser speckle contrast imaging (LSCI) [1][2][3][4][5], laser doppler flowmetry (LDF) [6][7][8], near-infrared (NIR) spectroscopy [9,10], and optical coherence tomography [11][12][13][14][15][16][17][18] to detect the vascular responses to brain such as the changes in cerebral blood flow (CBF) [1,5], cerebral blood volume (CBV) [3,4], deoxyhemoglobin concentration (HbR) and oxyhemoglobin (Hb-O2) [1,2,4]. These hemodynamic-related changes are used as the markers to reveal the neural response regions.…”

Section: Introductionmentioning

confidence: 99%

Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography

Tang¹,

Li²,

Rakymzhan³

et al. 2020

Biomed. Opt. Express

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We describe a method to measure tissue dynamics in mouse barrel cortex during functional activation via phase-sensitive optical coherence tomography (PhS-OCT). The method measures the phase changes in OCT signals, which are induced by the tissue volume change, upon which to localize the activated tissue region. Phase unwrapping, compensation and normalization are applied to increase the dynamic range of the OCT phase detection. To guide the OCT scanning, intrinsic optical signal imaging (IOSI) system equipped with a green light laser source (532 nm) is integrated with the PhS-OCT system to provide a full field time-lapsed images of the reflectance that is used to identify the transversal 2D localized tissue response in the mouse brain. The OCT results show a localized decrease in the OCT phase signal in the activated region of the mouse brain tissue. The decrease in the phase signal may be originated from the brain tissue compression caused by the vasodilatation in the activated region. The activated region revealed in the cross-sectional OCT image is consistent with that identified by the IOSI imaging, indicating the phase change in the OCT signals may associate with the changes in the corresponding hemodynamics. In vivo localized tissue dynamics in the barrel cortex at depth during whisker stimulation is observed and monitored in this study.

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Cortical Hemodynamic Responses Under Focused Ultrasound Stimulation Using Real-Time Laser Speckle Contrast Imaging

Cited by 20 publications

References 34 publications

On the neuromodulatory pathways of the in vivo brain by means of transcranial focused ultrasound

On the neuromodulatory pathways of the in vivo brain by means of transcranial focused ultrasound

Low-Intensity Pulsed Ultrasound Stimulation Modulates the Nonlinear Dynamics of Local Field Potentials in Temporal Lobe Epilepsy

Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography

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