Cortical cultures coupled to Micro-Electrode Arrays: A novel approach to perform in vitro excitotoxicity testing

Frega, Monica; Pasquale, Valentina; Tedesco, Mariateresa; Marcoli, Manuela; Contestabile, Andrea; Nanni, Marina; Bonzano, Laura; Maura, Guido; Chiappalone, Michela

doi:10.1016/j.ntt.2011.08.001

Cited by 93 publications

(77 citation statements)

References 55 publications

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“…NMDA also caused a biphasic response in MFR (Fig. 3B), in agreement with published results (Frega et al, 2012).…”

Section: Effects Of Glufosinate On Nmdar: [ 3 H]cgp 39653 Binding Inhsupporting

confidence: 92%

Glufosinate binds N-methyl-d-aspartate receptors and increases neuronal network activity in vitro

et al. 2014

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“…NMDA also caused a biphasic response in MFR (Fig. 3B), in agreement with published results (Frega et al, 2012).…”

Section: Effects Of Glufosinate On Nmdar: [ 3 H]cgp 39653 Binding Inhsupporting

confidence: 92%

Glufosinate binds N-methyl-d-aspartate receptors and increases neuronal network activity in vitro

et al. 2014

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“…However, using specific agonists and antagonists of a receptor, including NMDAR, MEA technology can be used to measure evoked activity, including glutamatergic receptor function . For example it has been shown that MEA-coupled neuronal cortical networks are very sensitive to pharmacological manipulation of the excitatory ionotropic glutamatergic transmission (Frega et al, 2012). MEAs can also be applied in higher throughput platforms to facilitate screening of numerous chemical compounds (McConnell et al, 2012).…”

Section: How It Is Measured or Detectedmentioning

confidence: 99%

Adverse Outcome Pathway on binding of agonists to ionotropic glutamate receptors in adult brain leading to excitotoxicity that mediates neuronal cell death, contributing to learning and memory impairment

Sachana

Munn

Bal‐Price

2016

OECD Series on Adverse Outcome Pathways

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“…Plate neuronal cultures on MEA chips, as described 29 . The description of a similar procedure is also provided 9 and in the Discussion section.…”

Section: Preparation Of Neuronal Culture Over a Meamentioning

confidence: 99%

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

Tessadori

Chiappalone

2015

JoVE

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Information coding in the Central Nervous System (CNS) remains unexplored. There is mounting evidence that, even at a very low level, the representation of a given stimulus might be dependent on context and history. If this is actually the case, bi-directional interactions between the brain (or if need be a reduced model of it) and sensory-motor system can shed a light on how encoding and decoding of information is performed. Here an experimental system is introduced and described in which the activity of a neuronal element (i.e., a network of neurons extracted from embryonic mammalian hippocampi) is given context and used to control the movement of an artificial agent, while environmental information is fed back to the culture as a sequence of electrical stimuli. This architecture allows a quick selection of diverse encoding, decoding, and learning algorithms to test different hypotheses on the computational properties of neuronal networks.

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Cortical cultures coupled to Micro-Electrode Arrays: A novel approach to perform in vitro excitotoxicity testing

Cited by 93 publications

References 55 publications

Glufosinate binds N-methyl-d-aspartate receptors and increases neuronal network activity in vitro

Glufosinate binds N-methyl-d-aspartate receptors and increases neuronal network activity in vitro

Adverse Outcome Pathway on binding of agonists to ionotropic glutamate receptors in adult brain leading to excitotoxicity that mediates neuronal cell death, contributing to learning and memory impairment

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

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