Cortical Aquaporin-4 in relation to brain oedema and neurological function of cortical cryo-injured mice

Gandham, Edmond Jonathan; Prabhakaran, Vasudevan; Moorthy, Ranjith K; Narasimhan, Kesavan; Murthy, M. Shankara; Rebekah, Grace; Rajshekhar, Vedantam

doi:10.1016/j.jocn.2017.05.001

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…Aquaporin 4 belonged to the aquaporin water channel family (Jung et al, 1994;Beitz and Schultz, 1999) and mainly abundant in brain astrocytes, capillary endothelial cells, and ependymal cells, and was prominently expressed in brain area including the supraoptic nucleus, hippocampal dentate gyrus, and cerebellum (Yool, 2007;Vandebroek and Yasui, 2020). AQP4 played an important role in regulating water balance in brain tissue, contributed to the reabsorption of cerebrospinal fluid and osmotic equilibrium, and closely related to the occurrence of cerebral edema (Papadopoulos et al, 2002;Gandham et al, 2017;Jullienne et al, 2018;Hindeya Gebreyesus and Gebrehiwot Gebremichael, 2020). Previous results showed that the water permeability of astrocytes was significantly decreased after the knockout of AQP4 and reduced the occurrence of cerebral edema (Manley et al, 2000;Papadopoulos and Verkman, 2005;Fu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Serum Metabolomics Associating With Circulating MicroRNA Profiles Reveal the Role of miR-383-5p in Rat Hippocampus Under Simulated Microgravity

et al. 2020

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Microgravity impacts various aspects of human health. Yet the mechanisms of spaceflight-induced health problems are not elucidated. Here, we mapped the fusion systemic analysis of the serum metabolome and the circulating microRNAome in a hindlimb unloading rat model to simulate microgravity. The response of serum metabolites and microRNAs to simulated microgravity was striking. Integrated pathway analysis of altered serum metabolites and target genes of the significantly altered circulating miRNAs with Integrated Molecular Pathway-Level Analysis (IMPaLA) software was mainly suggestive of modulation of neurofunctional signaling pathways. Particularly, we revealed significantly increased miR-383-5p and decreased aquaporin 4 (AQP4) in the hippocampus. Using rabies virus glycoprotein-modified exosomes, delivery of miR-383-5p inhibited the expression of AQP4 not only in rat C6 glioma cells in vitro but also in the hippocampus in vivo. Using bioinformatics to map the crosstalk between the circulating metabolome and miRNAome could offer opportunities to understand complex biological systems under microgravity. Our present results suggested that the change of miR-383-5p level and its regulation of target gene AQP4 was one of the potential molecular mechanisms of microgravity-induced cognitive impairment in the hippocampus.

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