Cortical and Papillary Micropuncture Examination of Chloride Transport in Segments of the Rat Kidney during Inhibition of Prostaglandin Production

Higashihara, Eiji; Stokes, John B.; Kokko, Juha P.; Campbell, William B.; DuBose, Thomas D.

doi:10.1172/jci109583

Cited by 99 publications

(26 citation statements)

References 18 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The left femoral artery was cannulated to monitor blood pressure and for collection of arterial blood samples. The left kidney was exposed by a flank incision and prepared for micropuncture of the renal cortex as previously described (8)(9)(10).…”

Section: Methodsmentioning

confidence: 99%

“…This was accomplished by collecting paired samples of tubule fluid from an early and late segment ofthe same PCT. Tubule fluid samples were collected for timed intervals (2.5-6 min) using sharpened 8-10-,gm OD glass pipettes as previously described (8,9). In each tubule, the late proximal segment was punctured before the early proximal segment to prevent leakage of tubule fluid.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Ammonia transport by early and late proximal convoluted tubule of the rat.

Good¹,

DuBose²

1987

J. Clin. Invest.

View full text Add to dashboard Cite

Free-flow micropuncture experiments were performed to examine ammonia transport separately in early and late proximal convoluted tubule (PCT) of the rat. In control rats, ammonia was secreted along the early PCT but was reabsorbed along the late PCT. In rats with chronic metabolic acidosis, ammonia secretion along the early PCI was increased compared with controls, and ammonia absorption by the late PCT was converted to small net ammonia secretion. In the acidotic rats, ammonia secretion rate in the early PCT was six times higher than that in the late PCT. Thus, most or all of ammonia secretion by the PCI occurred along its early portion. In control and acidotic rats, luminal NH3 concentration in the early PCT was significantly higher than that in the late PCI, indicating that ammonia is not in diffusion equilibrium throughout the renal cortex. It is proposed that differences in ammonia transport rate in early vs. late PCI? may be due to differences in ammonia production rate and/or to differences in the rate of an ammonia backflux that detracts from net ammonia secretion.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ammonia transport by early and late proximal convoluted tubule of the rat.

Good¹,

DuBose²

1987

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Although it has been suggested that the site(s) for this process might include juxtamedullary proximal tubules, the loops of Henle, or more distal segments (6) (12,13). Transit times > 13 s or a mean arterial blood pressure < 100 mm Hg were cause for rejection at this point.…”

Section: Introductionmentioning

confidence: 92%

“…Acid-base status of the rat was monitored and carefully maintained exactly as previously described (14,15). Micropuncture of the desired cortical or papillary nephron segment was accomplished with sharpened micropipettes containing Sudan-colored mineral oil exactly as previously described in detail (12,13). Descending or ascending vasa recta samples were collected in some of the groups noted below by the technique described by Lacy (17) immediately after collection as previously described (14).…”

Section: Introductionmentioning

confidence: 99%

Effect of carbonic anhydrase inhibition on superficial and deep nephron bicarbonate reabsorption in the rat.

DuBose¹,

Lucci²

1983

J. Clin. Invest.

View full text Add to dashboard Cite

“…In rat micropuncture experiments, Higashihara et al (41) have demonstrated a decrease in NaCl delivery to distal tubule sites and an increase in papillary NaCl PGE2 Effects on Cl-Absorption in mTALH content following prostaglandin synthesis inhibition; they concluded that prostaglandins might inhibit NaCl transport in the TALH. Complementary studies conducted by Kaukker (42), using micropuncture techniques, and by Stokes (43), using the isolated, perfused rabbit mTALH, have also been consistent with the notion that PGE2 inhibits NaCl absorption by the TALH.…”

mentioning

confidence: 98%

Interactions among prostaglandin E2, antidiuretic hormone, and cyclic adenosine monophosphate in modulating Cl- absorption in single mouse medullary thick ascending limbs of Henle.

Culpepper¹,

Andreoli²

1983

J. Clin. Invest.

109

View full text Add to dashboard Cite

A B S T R A C T This paper describes the inhibitory effect of prostaglandin E2 (PGE2) on antidiuretic hormone (ADH)-stimulated net Cl-absorption and spontaneous transepithelial voltage (Ve) in single medullary thick ascending limbs of Henle (TALH, thick ascending limb; mTALH, medullary segment; cTALH, cortical segment) obtained from mouse kidney. The experimental data indicate that PGE2 reduced the ADHdependent values of net Cl-absorption (Je,', eq cm-2 s-') and Ve (mV) in a dose-dependent manner; that increasing concentrations of peritubular ADH reversed the PGE2-mediated reductions in the ADH-dependent moiety of Ve in the mouse mTALH; that PGE2 had no effect on cyclic AMP-stimulated increments in Ve in the mouse mTALH; and that PGE2 had no In the renal medulla, this process enriches medullary osmolality, thereby contributing to urinary concentrating power (13,14). In certain species, like the mouse, salt absorption (11) and adenyl cyclase activity (15,16) in the water permeability of mammalian collecting ducts.In the isolated mouse mTALH, NaCl absorption involves the coupled entry of Cl-, and possibly K+ (17, 18), to Na+ entry across apical plasma membranes. In other words, Cl-transport in this nephron segment is a secondary active transport process, driven in part by the Na+ gradient between luminal fluid and cell interior (11,17). Conservative NaCl absorption in this nephron segment is enhanced considerably, and in a dose-dependent manner, by ADH, operating through the second messenger cyclic (c)AMP (11). Conversely, conservative NaCl absorption in the mouse mTALH is suppressed significantly by increases in peritubular osmolality independently of transepithelial osmotic pressure gradients (19).The mouse mTALH, like the rabbit cTALH (6, 20) and mTALH (7), behaves as a hybrid epithelium: the mouse mTALH is electrically leaky (11, 17) and rather permeable to Na+ and to 17), yet is virtually water impermeable either in the presence or absence of ADH (9-11). Consequently, as medullary peritubular salt concentrations vary, there occur varying degrees of dissipative NaCl backleak from interstitium to lumen through the paracellular pathway. Thus, for the mouse mTALH, maintenance of diluting power in the presence of varying NaCl concentrations in peritubular media depends, at least in part, on the interplay between ADH and peritubular osmolality in modifying conservative NaCl transport (11, 19). We will argue in this paper that PGE2 represents yet another factor that modulates the latter in the mouse mTALH.In the amphibian urinary bladder, Orloff et al. (21) found that prostaglandins inhibited the hydro6smotic effect of ADH but did not change the response of the epithelium to cAMP. They inferred that prostaglandins had acted at a locus proximal to hormone-dependent accumulation of cAMP within the cell, with little or no discernible prostaglandin action on transport events beyond cAMP accumulation. Based on in vitro studies in the isolated rabbit collecting duct, Grantham and Orloff (22) also suggested that prosta...

show abstract

Cortical and Papillary Micropuncture Examination of Chloride Transport in Segments of the Rat Kidney during Inhibition of Prostaglandin Production

Abstract: A B S T R A C T Prostaglandins

Cited by 99 publications

References 18 publications

Ammonia transport by early and late proximal convoluted tubule of the rat.

Ammonia transport by early and late proximal convoluted tubule of the rat.

Effect of carbonic anhydrase inhibition on superficial and deep nephron bicarbonate reabsorption in the rat.

Interactions among prostaglandin E2, antidiuretic hormone, and cyclic adenosine monophosphate in modulating Cl- absorption in single mouse medullary thick ascending limbs of Henle.

Contact Info

Product

Resources

About