Cortical alveolus breakdown in the eggs of the freshwater teleost <i>Rhodeus ocellatus ocellatus</i>

Ohta, Tatsuo; Isobe, Takashi; Tanaka, Masako; Yoshimoto, Yasuaki

doi:10.1002/ar.1092270412

Cited by 19 publications

(18 citation statements)

References 32 publications

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“…In teleosts, the simultaneous presence in an oocyte of ultrastructurally different cortical alveoli has been demonstrated (Ohta et al, 1990). In swordfish, on the basis of affinity to Pas reaction and abSal-Zrp immunoreactivity, at least two types of cortical alveoli seem to be present.…”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)

Corriero

Acone²,

Desantis³

et al. 2009

Eur J Histochem

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The paper reports a histological and immunohistochemical description of oocyte growth and ultrastructural aspects of zona radiata (ZR) formation as well as the relationship between plasma estradiol-17b‚ (E2) levels and ovarian development in swordfish (Xiphias gladius L.) from the Mediterranean Sea. Ovaries were inactive during March to mid April; maturation occurred during late April to June and spawning in June and July. Zona radiata formation starts, as Pas positive material, in oocytes at the lipid stage. In this stage a deposit of electrondense material between oolemma and follicular cells appears. In the cortical alveoli stage and through the early vitellogenic stage, the deposition of a moderately electrondense material occurred on the inner side of the ZR. Finally, in late vitellogenic oocytes a third layer, made of microfibrillar material, appeared. The immunohistochemical analyses revealed that the initial internalisation of hepatic zona radiata proteins (Zrp) in the swordfish oocyte starts before the uptake of vitellogenin (Vtg) and that it is associated with the low previtellogenic E2 plasma levels, while a significant E2 increase in plasma is associated with the beginning of Vtg uptake. This would appear to confirm the hypothesis that the differential and sequential induction of zonagenesis and vitellogenesis may reflect a general feature of teleost oogenesis

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Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)

Corriero

Acone²,

Desantis³

et al. 2009

Eur J Histochem

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“…Recent studies using lectins of different specificities revealed that the content of cortical alveoli in several species of notothenioid fish is variable (Motta et al 2005). In addition to species-specific variations in cortical alveoli content, the composition of cortical vesicles may also vary during oocyte development within a single (Ohta et al 1990). It is known that during fertilization, cortical alveoli fuse with the plasma membrane, releasing their content into the perivitelline space, thereby creating a barrier to polyspermy in fish (Yamamoto, 1956).…”

Section: B C D E Fmentioning

confidence: 99%

“…The same feature could be observed in our hybrids, since the rupture of some cortical alveoli (cortical reaction) and the formation of a small perivitelline space were detected at the moment of extrusion and prior the fertilization. According to Ohta et al (1990), oocytes become activated by a fertilizing spermatozoon or by the addition of freshwater. Some reports indicate, either explicitly or implicitly, that the cortical reaction should be the first detectable response in the oocyte to spermato-zoon fusion (Selman and Wallace, 1989).…”

Section: B C D E Fmentioning

confidence: 99%

Structural and ultrastructural characterization of the embryonic development of Pseudoplatystoma spp. hybrids

Faustino

Nakaghi²,

Marques³

et al. 2010

Int. J. Dev. Biol.

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The hybrid fish Pseudoplatystoma spp. has been raised on a large scale by several fish farmers, despite the fact that little is known about its biology. This is because it presents a number of zootechnical advantages over the parental species. In order to provide information about the early morphology of this important species, we analyzed the fertilization and embryonic development of the hybrid between spotted females and barred males of sorubim specimens by light microscopy and by scanning (SEM) and transmission electron microscopy (TEM) after induced spawning. Samples were collected at pre-established moments up to larval hatching. Seven distinct stages of hybrid embryonic development were identified: zygote, cleavage, morula, blastula, gastrula, histogenesis and organogenesis, and hatching. Under SEM, we observed spermatozoa at the micropyle entrance, the formation of a fertilization cone in the eggs, the differentiation of cephalic and caudal regions, the neural tube and embryo growth along the cephalo-caudal axis, as well as rudimentary optic vesicle and barbels. Under light microscopy, cytoplasmic movement was apparent with the consequent formation of animal and vegetative poles in eggs, in addition to epiboly movements and a small notochord portion. Under TEM, the oocyte chorion and eggs presented a sieve-like aspect in transversal cuts, coupled with the rupture of cortical alveoli and chorion elevation, thus enlarging the perivitelline space. Several mitochondria in the cortical cytoplasm were detected in both oocytes and eggs. Overall, we observed that the larvae hatched without visible morphological alterations, and seemed to be as viable in captive systems as they are in the natural environment.

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“…wave occurs in an egg, which is then followed by a cortical reaction (Ohta et al 1990;Lee et al 1999;Coward et al 2002;_ Zarski et al 2012b). However, the mechanism which triggers this Ca 2?…”

Section: Discussionmentioning

confidence: 99%

Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs

et al. 2013

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Within the study, the in vitro fertilization (ivF) in crucian carp, Carassius carassius (L.), with the use of three activating solutions (AS) [reverse osmosis water (RO), Woynarovich (WS) and Billard (BS) solutions] as well as the period of the capacity of eggs to be fertilized (up to 180 s post-egg-activation) in those solutions. Moreover, CASA analysis of sperm motility was conducted with each AS. In control groups (0 s), the highest (P \ 0.05) fertilization rate (93.2 %) was observed with WS. The application of RO and BS affected lower (P \ 0.05) embryo survival (63.5 and \5 %, respectively). High fertilization rate (over 90 %) was recorded up to 30 (RO) and 90 s (WS) post-egg-activation. Progressive sperm motility (pMOT) was high (over 48 %) in all treatment groups up to 30 s. In RO and WS groups, pMOT significantly decreased (P \ 0.05) after 45 s post activation. BS affected pMOT over 10 % up to the 165 s. No differences (P [ 0.05) between the groups were found considering the curvilinear velocity (VCL) 15 s postsperm-activation. Between 30 and 135 s, the lowest (P \ 0.05) VCL after application of RO was noted. The highest VCL (P \ 0.05) up to 135 s was noted after application of BS and WS. All AS activated crucian carp sperm. The lowest fertilization rate was noted when BS was used, despite the high pMOT and VCL. It suggest that the AS which activates the sperm properly may not activate the eggs probably due to high osmolality of BS (262 mOsm kg -1 ). Because eggs retained the longest period of activity in WS, this AS is suggested for ivF in crucian carp.

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Cortical alveolus breakdown in the eggs of the freshwater teleost Rhodeus ocellatus ocellatus

Cited by 19 publications

References 32 publications

Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)

Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)

Structural and ultrastructural characterization of the embryonic development of Pseudoplatystoma spp. hybrids

Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs

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