Corrosion of API XL 52 steel in presence of <i>Clostridium celerecrescens</i>

Monroy, O. A. Ramos; Gayosso, M. J. Hernández; Ruiz‐Ordaz, Nora; Olivares, Gerardo Zavala; Ramírez, Cleotilde Juárez

doi:10.1002/maco.200905582

Cited by 15 publications

(7 citation statements)

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“…Clostridia of the genus Clostridium were best represented in this enrichment’s metagenome after four transfers (36%, Supplementary Figure S5 ) and persisted in the enrichments after 11 transfers. Clostridium includes several species of CO 2 -reducing acetogens that use H 2 as an electron donor ( Bengelsdorf et al, 2018 ) but also electrodes ( Nevin et al, 2011 ) and Fe 0 ( Monroy et al, 2011 ). Besides, Clostridium was previously enriched on Fe 0 from environments such as scraped bicycles thrown in a Dutch channel ( Philips et al, 2019 ), rice paddies ( Kato et al, 2015 ), or oilfield production waters ( Ma et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

2021

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Diverse physiological groups congregate into environmental corrosive biofilms, yet the interspecies interactions between these corrosive physiological groups are seldom examined. We, therefore, explored Fe0-dependent cross-group interactions between acetogens and methanogens from lake sediments. On Fe0, acetogens were more corrosive and metabolically active when decoupled from methanogens, whereas methanogens were more metabolically active when coupled with acetogens. This suggests an opportunistic (win–loss) interaction on Fe0 between acetogens (loss) and methanogens (win). Clostridia and Methanobacterium were the major candidates doing acetogenesis and methanogenesis after four transfers (metagenome sequencing) and the only groups detected after 11 transfers (amplicon sequencing) on Fe0. Since abiotic H2 failed to explain the high metabolic rates on Fe0, we examined whether cell exudates (spent media filtrate) promoted the H2-evolving reaction on Fe0 above abiotic controls. Undeniably, spent media filtrate generated three- to four-fold more H2 than abiotic controls, which could be partly explained by thermolabile enzymes and partly by non-thermolabile constituents released by cells. Next, we examined the metagenome for candidate enzymes/shuttles that could catalyze H2 evolution from Fe0 and found candidate H2-evolving hydrogenases and an almost complete pathway for flavin biosynthesis in Clostridium. Clostridial ferredoxin-dependent [FeFe]-hydrogenases may be catalyzing the H2-evolving reaction on Fe0, explaining the significant H2 evolved by spent media exposed to Fe0. It is typical of Clostridia to secrete enzymes and other small molecules for lytic purposes. Here, they may secrete such molecules to enhance their own electron uptake from extracellular electron donors but indirectly make their H2-consuming neighbors—Methanobacterium—fare five times better in their presence. The particular enzymes and constituents promoting H2 evolution from Fe0 remain to be determined. However, we postulate that in a static environment like corrosive crust biofilms in lake sediments, less corrosive methanogens like Methanobacterium could extend corrosion long after acetogenesis ceased, by exploiting the constituents secreted by acetogens.

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Section: Resultsmentioning

confidence: 99%

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

2021

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“…Members of Clostridium genus were also identi ed in our analyses. They have been reported in environmental corrosive bio lms by other studies as well [21,39]. Monroy and colleagues [39] detected lower corrosion rates for Clostridium celerecrescens than that corrosion rate for SRB.…”

Section: Discussionmentioning

confidence: 74%

Corrosion of Weld Heat-Affected Zones Submersed in Seawater with Indigenous Microorganisms

et al. 2023

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The corrosion behaviors of different weld heat-affected zones (HAZs) were investigated, comprising an API 5L X65 steel immersed in seawater with an abundant native microbial community. Before tests, HAZ physical simulations were performed at the peak temperatures of 700°C, 860°C, 1000°C, and 1350°C. Throughout 14 days of immersion, various planktonic bacterial groups associated with microbiologically in uenced corrosion were metabolically active in the seawater. Desulfovibrio members were present in a considerable number of OTUs. Scanning electron microscopy showed an intense microbial adhesion and corrosion product deposits in all HAZ sub-regions and the control base metal coupon (BM). The electrochemical tests revealed that the corrosion behavior of all HAZ sub-regions and BM were indistinguishable. However, topography analysis showed different behaviors of pitting corrosion. The results revealed that the pitting resistance of the microstructure composed of cementite grains (700°C HAZ) was superior to that of other HAZ sub-regions. On the other hand, the microstructure composed of martensite and austenite grains (860°C HAZ) exhibited the worst pitting resistance of the weld joint, leading to a maximum pit penetration of 2.3 times greater than that pit on the BM. The changes in the HAZ sub-region sensitivity to pitting corrosion were attributed to variations in the microstructural composition.

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“…7a) and the best coverage of the Wood-Ljungdahl pathway for autotrophic acetogenesis (Fig.7, Table 1). Clostridium includes several species of autotrophs that use H 2 as electron donor (Bengelsdorf et al, 2018) but also electrodes (Nevin et al, 2011) and Fe 0 (Monroy et al, 2011). Besides, Clostridium was previously enriched on Fe 0 from environments such as scraped bicycles thrown in a Dutch channel (Philips et al, 2019), rice paddies (Kato and Yumoto, 2015) or oilfield production waters (Ma et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Opportunistic interactions on Fe⁰ between methanogens and acetogens from a climate lake

Rotaru

2019

Preprint

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10Urban environments are webbed with iron-steel structures above and belowground. Underground, in non-11 sulfidic environments, it has been suggested that interspecies interactions cause Fe 0 corrosion. Particularly, 12Methanosarcinales were assumed to interact syntrophically with acetogenic bacteria during Fe 0 corrosion. 13Here we challenge this assumption and show that a community of methanogens (38% Methanosarcinales) 14 prospers on Fe 0 especially after the demise of the acetogens. Acetogens were mainly represented by 15Clostridium (81% of Bacteria). Methanogens were however more diverse including Methanosarcina (22% of 16 Archaea), Methanosaeta (17% of Archaea) and Methanothermobacter (22% of Archaea) as key groups. 17 Surprisingly, acetogens started using electrons from Fe 0 immediately, unchallenged by competing 18 methanogens. Acetogens were expected to be outcompeted by energy efficient methanogens with 19 comparatively lower H2-uptake thresholds. However, acetogens prevailed, perhaps because in contrast to 20 methanogens they contain [FeFe]-hydrogenases (encoded in the lake-Clostridia metagenome).[FeFe]-21 hydrogenases from Clostridium were previously shown effective at retrieving electrons from Fe 0 for proton 22

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Corrosion of API XL 52 steel in presence of Clostridium celerecrescens

Cited by 15 publications

References 3 publications

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

Corrosion of Weld Heat-Affected Zones Submersed in Seawater with Indigenous Microorganisms

Opportunistic interactions on Fe⁰ between methanogens and acetogens from a climate lake

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Corrosion of API XL 52 steel in presence of Clostridium celerecrescens

Cited by 15 publications

References 3 publications

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

Corrosion of Weld Heat-Affected Zones Submersed in Seawater with Indigenous Microorganisms

Opportunistic interactions on Fe0 between methanogens and acetogens from a climate lake

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Opportunistic interactions on Fe⁰ between methanogens and acetogens from a climate lake