Corrinoid‐Dependent Methyl Transfer Reactions in<i>Sporomusa ovata</i>

Stupperich, Erhard; Konle, Ralph; Lehle, Michaela

doi:10.1002/9783527612192.ch10

Cited by 6 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some reports showed O-demethylation by oxygenase or by tetrahydrofolate (THF)-dependent methyltransferase. [15][16][17][18][19][20][21] The importance of THF and ATP in the anaerobic O-demethylation was reported. 22) Here, we investigated effects of THF and ATP on demethylation of arctigenin by E. sp.…”

mentioning

confidence: 99%

Isolation and Characterization of a Human Intestinal Bacterium, Eubacterium sp. ARC-2, Capable of Demethylating Arctigenin, in the Essential Metabolic Process to Enterolactone

Jin

Zhao

Nakamura

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Plant lignans, such as pinoresinol diglucoside, secoisolariciresinol diglucoside and arctiin, are metabolized to mammalian lignans, enterolactone or enterodiol, by human intestinal bacteria. Their metabolic processes include deglucosylation, ring cleavage, demethylation, dehydroxylation and oxidation. Here we isolated an intestinal bacterium capable of demethylating arctigenin, an aglycone of arctiin, to 2,3-bis(3,4-dihydroxybenzyl)butyrolactone (1) from human feces, and identified as an Eubacterium species (E. sp. ARC-2), which is similar to Eubacterium limosum on the basis of morphological and biochemical properties and 16S rRNA gene sequencing. By incubating with E. sp. ARC-2, arctigenin was converted to 1 through stepwise demethylation. Demethylation of arctigenin by E. sp. ARC-2 was tetrahydrofolate-and ATP-dependent, indicating that the reaction was catalyzed by methyltransferase. Moreover, E. sp. ARC-2 transformed secoisolariciresinol to 2,3-bis(3,4-dihydroxybenzyl)-1,4-butanediol by demethylation.

show abstract

mentioning

confidence: 99%