Corrigendum to “Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases” [Synth Syst Biotechnol 5 (2) (2020) 62–80]

Drufva, Erin E.; Hix, Elijah G.; Bailey, Constance B.

doi:10.1016/j.synbio.2020.07.007

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“…In this review, we comprehensively compile and discuss the results of these studies, primarily focusing on excised KRs in vitro. As PKSs are complex enzymes with multiple nuanced protein–protein interactions mediating their assembly, there is considerable interest in altering domain selectivity through methods that are minimally disruptive to these critical protein–protein interfaces [42] . Success has been observed with domain exchange [43,44] or whole module swapping as a means to alter stereochemical configuration in intact PKS modules, and admittedly, such an approach has been far more successful than site‐directed mutagenesis to alter KR stereocontrol in intact modules to date.…”

Section: Introductionmentioning

confidence: 99%

Site‐Directed Mutagenesis of Modular Polyketide Synthase Ketoreductase Domains for Altered Stereochemical Control

et al. 2020

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Bacterial modular type I polyketide synthases (PKSs) are complex multidomain assembly line proteins that produce a range of pharmaceutically relevant molecules with a high degree of stereochemical control. Due to their colinear properties, they have been considerable targets for rational biosynthetic pathway engineering. Among the domains harbored within these complex assembly lines, ketoreductase (KR) domains have been extensively studied with the goal of altering their stereoselectivity by site‐directed mutagenesis, as they confer much of the stereochemical complexity present in pharmaceutically active reduced polyketide scaffolds. Here we review all efforts to date to perform site‐directed mutagenesis on PKS KRs, most of which have been done in the context of excised KR domains on model diffusible substrates such as β‐keto N‐acetyl cysteamine thioesters. We also discuss the challenges around translating the findings of these studies to alter stereocontrol in the context of a complex multidomain enzymatic assembly line.

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Section: Introductionmentioning

confidence: 99%