Corrigendum to “Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants” [Acta Biomaterialia 69 (2018) 120–130]

Jangamreddy, Jaganmohan Reddy; Haagdorens, Michel; Islam, Mohammad Mirazul; Lewis, Philip N.; Samanta, Ayan; Liszka, Aneta; Ljunggren, Monika Kozak; Buznyk, Oleksiy; Alarcon, Emilio I.; Zakaria, Nadia; Meek, Keith Michael Andrew; Griffith, May

doi:10.1016/j.actbio.2018.10.009

Cited by 4 publications

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“…In our experiments, surface modification of RHC I did not influence pattern of cell proliferation, which might be attributed to the inherent biocompatibility of RHC [18–21, 27, 31, 32, 57, 58]. Even though surface modification of CLP DMTMM hydrogels influenced the pattern of cell proliferation, the rate of outgrowth and the genotype and phenotype of cultured cells were not significantly altered.…”

Section: Discussionmentioning

confidence: 49%

“…Interestingly, our previous data indicate that CLP-12 EDC might be a suitable acellular alternative to conventional corneal transplantion [27]. In the work of Jangamreddy et al, we noted that suboptimal growth of iHCECs at days 1 and 2 on CLP-12 EDC hydrogels does not necessarily translate into inferior in vivo performance [27]. After all, acellular CLP-12 EDC corneas supported functional reepithelialization when implanted in pigs.…”

Section: Discussionmentioning

confidence: 90%

“…CLP-12 EDC, however, was the only tested carrier material for which primary limbal epithelial cultures did not meet quality standards due to low cell yield. Interestingly, our previous data indicate that CLP-12 EDC might be a suitable acellular alternative to conventional corneal transplantion [27]. In the work of Jangamreddy et al, we noted that suboptimal growth of iHCECs at days 1 and 2 on CLP-12 EDC hydrogels does not necessarily translate into inferior in vivo performance [27].…”

Section: Discussionmentioning

confidence: 95%

“…CLP and RHC I both originate from xeno-free sources and are optically transparent, mechanically stable, and biocompatible in vivo [27, 28]. CLP-12 EDC hydrogels have shown great promise as an acellular corneal construct in vivo [27, 28]. However, EDC/NHS crosslinking is too fast to allow successful casting and production of thin hydrogels (<300 μ m thick) [52].…”

Section: Discussionmentioning

confidence: 99%

“…As a synthetic peptide, CLP makes room for ready and scaled-up production. When tested as a corneal construct in an animal model, CLP proved to be functionally equivalent to RHC III, except for mechanical strength for which CLP underperformed [27, 28]. These reports provide a proof-of-principle and indicate that it is worthwhile exploring the versatility of CLP hydrogels as a scaffold for LESC cultivation.…”

Section: Introductionmentioning

confidence: 93%

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In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

Haagdorens

Cėpla

Melsbach

et al. 2019

Stem Cells International

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Purpose. To investigate the efficacy of recombinant human collagen type I (RHC I) and collagen-like peptide (CLP) hydrogels as alternative carrier substrates for the cultivation of limbal epithelial stem cells (LESC) under xeno-free culture conditions. Methods. Human LESC were cultivated on seven different collagen-derived hydrogels: (1) unmodified RHC I, (2) fibronectin-patterned RHC I, (3) carbodiimide-crosslinked CLP (CLP-12 EDC), (4) DMTMM- (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium-) crosslinked CLP (CLP-12), (5) fibronectin-patterned CLP-12, (6) “3D limbal niche-mimicking” CLP-12, and (7) DMTMM-crosslinked CLP made from higher CLP concentration solution. Cell proliferation, cell morphology, and expression of LESC markers were analyzed. All data were compared to cultures on human amniotic membrane (HAM). Results. Human LESC were successfully cultivated on six out of seven hydrogel formulations, with primary cell cultures on CLP-12 EDC being deemed unsuccessful since the area of outgrowth did not meet quality standards (i.e., inconsistence in outgrowth and confluence) after 14 days of culture. Upon confluence, primary LESC showed high expression of the stem cell marker ΔNp63, proliferation marker cytokeratin (KRT) 14, adhesion markers integrin-β4 and E-cadherin, and LESC-specific extracellular matrix proteins laminin-α1, and collagen type IV. Cells showed low expression of differentiation markers KRT3 and desmoglein 3 (DSG3). Significantly higher gene expression of KRT3 was observed for cells cultured on CLP hydrogels compared to RHC I and HAM. Surface patterning of hydrogels influenced the pattern of proliferation but had no significant effect on the phenotype or genotype of cultures. Overall, the performance of RHC I and DMTMM-crosslinked CLP hydrogels was equivalent to that of HAM. Conclusion. RHC I and DMTMM-crosslinked CLP hydrogels, irrespective of surface modification, support successful cultivation of primary human LESC using a xeno-free cultivation protocol. The regenerated epithelium maintained similar characteristics to HAM-based cultures.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

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In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

Haagdorens

Cėpla

Melsbach

et al. 2019

Stem Cells International

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Response to Letter to Editor “Comment on “Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants” by Jangamreddy JR et al.”

Griffith

2019

Acta Biomaterialia

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Towards rationally designed biomanufacturing of therapeutic extracellular vesicles: impact of the bioproduction microenvironment

Patel

Santoro

Born

et al. 2018

Biotechnology Advances

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Extracellular vesicles (EVs), including exosomes, microvesicles, and others, have emerged as potential therapeutics for a variety of applications. Pre-clinical reports of EV efficacy in treatment of non-healing wounds, myocardial infarction, osteoarthritis, traumatic brain injury, spinal cord injury, and many other injuries and diseases demonstrate the versatility of this nascent therapeutic modality. EVs have also been demonstrated to be effective in humans, and clinical trials are underway to further explore their potential. However, for EVs to become a new class of clinical therapeutics, issues related to translation must be addressed. For example, approaches originally developed for cell biomanufacturing, such as hollow fiber bioreactor culture, have been adapted for EV production, but limited knowledge of how the cell culture microenvironment specifically impacts EVs restricts the possibility for rational design and optimization of EV production and potency. In this review, we discuss current knowledge of this issue and delineate potential focus areas for future research towards enabling translation and widespread application of EV-based therapeutics.

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Corrigendum to “Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants” [Acta Biomaterialia 69 (2018) 120–130]

Cited by 4 publications

References 0 publications

In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

Response to Letter to Editor “Comment on “Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants” by Jangamreddy JR et al.”

Towards rationally designed biomanufacturing of therapeutic extracellular vesicles: impact of the bioproduction microenvironment

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