Corrigendum to Rare codon priority and its position specificity at the 5′ of the gene modulates heterologous protein expression in Escherichia coli

Thangadurai, Chinnathambi; Suthakaran, Pichaimuthu; Barfal, Pankaj; Balaiah, Anandaraj; Pradhan, Satya Narayan; Buniya, Harith K.; Ramalingam, Subramanian; Murugan, Vadivel

doi:10.1016/j.bbrc.2021.05.001

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“…The transcription of genes is regulated by the binding of transcription factors to cisacting elements present in the promoter region. Previous studies on promoters of genes involved in starch biosynthesis have revealed that these cis-elements are active in the presence of specific signaling pathways [31,[44][45][46][47]. Analysis of the Bt1 gene promoter has predicted the presence of various cis-acting elements, suggesting its involvement in regulatory mechanisms (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…The most common and straightforward method to obtain antibodies is to immunize animals with antigens and extract the antiserum produced by them [29,30]. E. coli was used as a host due to its clear biological genetic information, high efficiency, simple living conditions, and importance in bacterial expression systems [31,32]. The pGEX-6p-ZmBT1-C prokaryotic expression vector was successfully constructed through RT-PCR, cloning, and double restriction enzyme digestion, and it could express high-quality ZmBT1-C recombinant protein.…”

Section: Discussionmentioning

confidence: 99%

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Preparation of Polyclonal Antibody against ZmBT1 Protein and Its Application in Hormone-Regulated Starch Synthesis

et al. 2023

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In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze the expression and distribution of ZmBT1 in various maize tissues, we prepared a polyclonal antibody. Specifically, we successfully expressed the recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) and purified Gst-ZmBT1-C as the antigen for antibody preparation. Our results confirmed that the ZmBT1 protein in maize tissues can be specifically recognized by the ZmBT1 antibody. Through Western blotting, we observed that the expression protein of ZmBT1 varied by tissues, with the highest content in the grain and endosperm. Furthermore, we employed a combination of Western blotting and quantitative real-time PCR to show that the expression level of ZmBT1 can be influenced by plant hormones. This finding suggests that ZmBT1 plays a critical role in the accumulation of starch and opens up new avenues for functional studies of this protein.

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