Corrigendum to “PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs” [Biochem. Biophys. Res. Commun. 373 (2008) 104–108]

Kim, Young‐Ju; Kim, Hye-Joung; Im, Gun‐Il

doi:10.1016/j.bbrc.2008.08.133

Cited by 2 publications

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“…To improve cartilage formation, in addition to manipulating the mechanical environment, culture of the HAC aggregates within the acoustic field was investigated in the presence of PTHrP, a growth factor with defined roles in promoting chondrogenesis and inhibiting hypertrophy, especially in presence of mechanical stimulation. [28][29][30] Notably, 21 day constructs generated in the bioreactor in chondrogenic media supplemented with PTHrP, using a sweep repetition rate of 2 Hz were: i) appreciably larger in size, ii) exhibited distinct hyaline-like cartilage structure, iii) demonstrated robust expression of SOX-9 and Type II collagen, and, importantly, iv) displayed negligible expression of collagens Type I and Type X. Additionally, mechanical analysis of the cartilage tissue using nano-indentation showed the engineered human cartilage constructs to have stiffness similar to that of native human cartilage tissue.…”

Section: Discussionmentioning

confidence: 99%

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Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

et al. 2018

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Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.

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Section: Discussionmentioning

confidence: 99%

“…26 Furthermore, mechanical stimulation of chondrocytes results in the secretion of parathyroid hormone-related protein (PTHrP), a chemical factor that has been found to aid chondrogenesis and reduce hypertrophy. [28][29][30] Thus, there is clear evidence that biomechanical stimulation of chondrocytes promotes chondrogenesis.…”

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confidence: 99%

Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

et al. 2018

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“…Osteoarthritis is a common disease, clinically manifested by joint pain, swelling, and impairment of joint function, which leads to disability and the need for joint replacement. Levels of β-catenin and cyclooxygenase 2 (COX2) were increased in osteoarthritic and rheumatoid arthritic (RA) cartilage, suggesting that accumulation of β-catenin may contribute to the inflammatory responses of cartilage by inducing COX2 expression in chondrocytes of arthritis-affected cartilage (Kim et al 2002).…”

Section: Crosstalk Of Mapk and Wnt Signals In Cartilage Inflammation mentioning

confidence: 99%

Decellularized matrix and cartilage regeneration: A focus on developing a matrix with anti-inflammation and micromechanical properties

Zhang¹

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Osteoarthritis (OA) is predicted to be the fourth leading cause of disability worldwide by the year 2020. Stem cell-based therapy is a promising biological approach for the treatment of cartilage defects. However, adult stem cells tend to become replicatively senescent when they are expanded on conventional plastic flasks. Decellularized extracellular matrix (dECM) provides an expansion system to rejuvenate and maintain the chondrogenic phenotype of stem cells for cartilage repair. In addition, joints with cartilage defects normally have inflammation to some extent; several stimuli, such as Reactive Oxygen Species (ROS) and Interleukin-1 (IL-1), reportedly induce apoptosis of chondrocytes. The tolerance of transplanted cells to a harsh environment is a key determinant for successful cartilage repair. dECM expansion gives adult stem cells an ability to resist inflammation and oxidants. This anti-inflammatory effect was further optimized by the combined use of small molecules in inflammation-related signaling pathways. Furthermore, dECM is a three-dimensional nanofiber structure with unique micromechanical properties. The influence of dECM elasticity on expanded stem cells' chondrogenic differentiation has been studied. Our long-term goal is to develop an in vitro stem cell matrix with optimized micromechanical properties to enhance stem cell proliferation and boost chondrogenic differentiation in order to treat cartilage defects and OA.

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Corrigendum to “PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs” [Biochem. Biophys. Res. Commun. 373 (2008) 104–108]

Cited by 2 publications

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Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

Decellularized matrix and cartilage regeneration: A focus on developing a matrix with anti-inflammation and micromechanical properties

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