Corrigendum to “Compositional and fluorescence characteristics of the giant diatom Ethmodiscus along a 3000 km transect (28°N) in the central North Pacific gyre”

Villareal, Tracy A.; McKay, R. Michael L.; Al-Rshaidat, Mamoon M. D.; Boyanapalli, Ramakrishna; Sherrell, Robert M.

doi:10.1016/j.dsr.2007.08.003

Cited by 10 publications

(14 citation statements)

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“…However, these differences can be largely explained if all organisms examined by all studies to date are classified as either neritic or oceanic. Prior to this study, 14 species of marine diatom had been examined for flavodoxin expression (La Roche et al 1995, Doucette et al 1996, McKay et al 2000, Davey and Geider 2001, Villareal et al 2007). Twelve of these produced flavodoxin when grown under iron-limited conditions and suppressed its expression completely when grown in an environment with sufficient iron.…”

Section: Resultsmentioning

confidence: 99%

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹

Pankowski

McMinn

2009

Journal of Phycology

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While the growth of Southern Ocean phytoplankton is often limited by iron availability, there are no comparable experiments on sea-ice algae. Here we assess the use of ferredoxin and flavodoxin to investigate the iron nutritional status of sea-ice algae and describe the development of a quantitative immunoassay for both proteins in marine diatoms. High-affinity monoclonal antibodies toward both proteins were produced from Cylindrotheca closterium (Ehrenb.) J. M. Lewin et Reimann, and these were used to develop Western blots. Western blots run on whole protein extracts detected both proteins with little cross-reactivity toward other proteins. The two proteins could be successfully quantitated when applied to gels at between 5 and 50 ng in a volume of 25 μL (0.2-2 μg · mL(-1) ). Flavodoxin and ferrodoxin expression was examined in the Antarctic diatoms Entomoneis kjellmannii (Cleve) Poulin et Cardinal, Navicula directa (W. Sm.) Ralfs, Fragilariopsis curta (Van Heurck) Hust., Pseudo-nitzschia sp., Porosira glacialis (Grunow) E. G. Jørg., Fragilariopsis cylindrus (Grunow) Willi Krieg., Fragilariopsis sublinearis (Van Heurck) Heiden et Kolbe, C. closterium, Nitzschia lecointei Van Heurck, and the dinoflagellate Polarella glacialis Montresor, Procaccini et Stoecker. Two Arctic isolates were also examined, Nitzschia frigida (Grunow) and Fragilariopsis oceanica (Cleve) Hasle. Significant heterogeneity of protein expression was observed despite all cultures being grown in iron-replete f/2 medium. Only one species, F. cylindrus, displayed the expected expression of ferredoxin only in iron-replete medium. Four were observed to produce both proteins under iron-replete conditions. Ferredoxin was not detected at all in F. curta and Pseudo-nitzschia sp., but distinct flavodoxin bands were observed in both of these organisms. All species examined were observed to express either flavodoxin or ferredoxin or both of the proteins as determined by Western immunoblotting.

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Section: Resultsmentioning

confidence: 99%

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹

Pankowski

McMinn

2009

Journal of Phycology

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“…Ethmodiscus rex is a widely-distributed warm-water diatom characteristic of oligotrophic open ocean water [15,16] . Villareal et al [17] believe that they have the positive buoyancy and vertical migration capacity and belong to "shade flora". Ethmodiscus can grow in low illumination conditions and cells descend below the nutricline for nitrogen (and possibly other macro-and micro-nutrients) and then return to the euphotic zone for photosynthesis.…”

Section: The Category Of Mat-forming Diatomsmentioning

confidence: 99%

Vast laminated diatom mat deposits from the west low-latitude Pacific Ocean in the last glacial period

Zhai

Chang

et al. 2009

Sci. Bull.

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“…Compared with cell volumes of 10 3 –10 5 µm 3 for spring‐bloom diatoms, E . rex has much large cell volumes (up to 10 9 µm 3 ) dominated by a single vacuole (>99% of total volume) [ Villareal et al ., , ]. Whereas spring‐bloom diatoms favor well‐mixed, fertile surface waters, E .…”

Section: Introductionmentioning

confidence: 99%

“…Recent advances in our knowledge of the ecology and biochemistry of E . rex [ Villareal , ; Villareal and Carpenter , ; Villareal et al ., , ] have yielded two new models for the formation of giant diatom LDMs. In the “ocean stratification model,” E .…”

Section: Introductionmentioning

confidence: 99%

“…As a typical giant diatom species, Ethmodiscus rex shows distinct physiological and ecological features that distinguish it from small spring-bloom diatoms. Compared with cell volumes of 10 3 -10 5 μm 3 for springbloom diatoms, E. rex has much large cell volumes (up to 10 9 μm 3 ) dominated by a single vacuole (>99% of total volume) [Villareal et al, 1999a[Villareal et al, , 2007. Whereas spring-bloom diatoms favor well-mixed, fertile surface waters, E. rex reaches maximum abundance within stable and stratified, oligotrophic water masses [Villareal et al, 1999a;Kemp et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

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The silicon isotope composition of Ethmodiscus rex laminated diatom mats from the tropical West Pacific: Implications for silicate cycling during the Last Glacial Maximum

Xiong

Algeo

et al. 2015

Paleoceanography

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The cause of massive blooms of Ethmodiscus rex laminated diatom mats (LDMs) in the easternPhilippine Sea (EPS) during the Last Glacial Maximum (LGM) remains uncertain. In order to better understand the mechanism of formation of E. rex LDMs from the perspective of dissolved silicon (DSi) utilization, we determined the silicon isotopic composition of single E. rex diatom frustules (δ 30 Si E. rex ) from two sediment cores in the Parece Vela Basin of the EPS. In the study cores, δ 30 Si E. rex varies from À1.23‰ to À0.83‰ (average À1.04‰), a range that is atypical of marine diatom δ 30Si and that corresponds to the lower limit of reported diatom δ 30Si values of any age. A binary mixing model (upwelled silicon versus eolian silicon) accounting for silicon isotopic fractionation during DSi uptake by diatoms was constructed. The binary mixing model demonstrates that E. rex dominantly utilized DSi from eolian sources (i.e., Asian dust) with only minor contributions from upwelled seawater sources (i.e., advected from Subantarctic Mode Water, Antarctic Intermediate Water, or North Pacific Intermediate Water). E. rex utilized only~24% of available DSi, indicating that surface waters of the EPS were eutrophic with respect to silicon during the LGM. Our results suggest that giant diatoms did not always use a buoyancy strategy to obtain nutrients from the deep nutrient pool, thus revising previously proposed models for the formation of E. rex LDMs.

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Corrigendum to “Compositional and fluorescence characteristics of the giant diatom Ethmodiscus along a 3000 km transect (28°N) in the central North Pacific gyre”

Cited by 10 publications

References 0 publications

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹

Vast laminated diatom mat deposits from the west low-latitude Pacific Ocean in the last glacial period

The silicon isotope composition of Ethmodiscus rex laminated diatom mats from the tropical West Pacific: Implications for silicate cycling during the Last Glacial Maximum

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Corrigendum to “Compositional and fluorescence characteristics of the giant diatom Ethmodiscus along a 3000 km transect (28°N) in the central North Pacific gyre”

Cited by 10 publications

References 0 publications

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE1

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE1

Vast laminated diatom mat deposits from the west low-latitude Pacific Ocean in the last glacial period

The silicon isotope composition of Ethmodiscus rex laminated diatom mats from the tropical West Pacific: Implications for silicate cycling during the Last Glacial Maximum

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DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹

DEVELOPMENT OF IMMUNOASSAYS FOR THE IRON‐REGULATED PROTEINS FERREDOXIN AND FLAVODOXIN IN POLAR MICROALGAE¹