Corrigendum to “Biochemical characterization of SARS-CoV-2 nucleocapsid protein”

Zeng, Weihong; Liu, Guangfeng; Ma, Huan; Zhao, Dan; Yu, Yang; Liu, Muziying; Mohammed, Ahmed; Zhao, Chi; Yang, Yun; Xie, Jiajia; Ding, Changming; Ma, Xiaoling; Weng, Jianping; Gao, Yong; He, Hongliang; Jin, Tengchuan

doi:10.1016/j.bbrc.2022.05.058

Cited by 1 publication

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“…Briefly, the spike protein (S) has S1 and S2 subunits, which recognizes the human receptor ACE-2 and mediates the viral membrane fusion with the host plasma membrane 4,5 . Whereas, the nucleocapsid protein (N) is phosphorylated and highly basic in nature, whose primarily function is associated with the packaging of viral genomic RNA 6,7 . The CoV's N protein contain two RNA-binding domains: the N-terminal domain and the C-terminal domain, linked by a serine/arginine-rich domain (SRD) [8][9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

An insight into SARS-CoV-2 membrane protein interaction with spike, envelope, and nucleocapsid proteins

Kumar

Garg

et al. 2021

Journal of Biomolecular Structure and Dynamics

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Intraviral protein-protein interactions are crucial for replication, pathogenicity, and viral assembly. Among these, virus assembly is a critical step as it regulates the arrangements of viral structural proteins and helps in the encapsulation of genomic material. SARS-CoV-2 structural proteins play an essential role in the self-rearrangement, RNA encapsulation, and mature virus particle formation. In SARS-CoV, the membrane protein interacts with the envelope and spike protein in Endoplasmic Reticulum Golgi Intermediate Complex (ERGIC) to form an assembly in the lipid bilayer, followed by membrane-ribonucleoprotein (nucleocapsid) interaction. In this study, we tried to understand the interaction of membrane protein's interaction with envelope, spike, and nucleocapsid proteins using protein-protein docking. Further, simulation studies performed up to 100 ns to examine the stability of protein-protein complexes of Membrane-Envelope, Membrane-Spike, and Membrane-Nucleocapsid. Prime MM-GBSA showed high binding energy calculations than the docked complex. The interactions identified in our study will be of great importance, as it provides valuable insight into the protein-protein complex, which could be the potential drug targets for future studies.

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