Corrigendum: Stoichiometric Shifts in Soil C:N:P Promote Bacterial Taxa Dominance, Maintain Biodiversity, and Deconstruct Community Assemblages

The Burning of Biocrusts Facilitates the Emergence of a Bare Soil Community of Poorly-Connected Chemoheterotrophic Bacteria With Depressed Ecosystem Services

“…Bacterial communities were evaluated from a composite surface soil sample from three subsamples (2 cm width × 2 mm depth) with a soil corer (2 fire treatments × 2 biocrust microsites × 3 time points × 3 replicates = 36 samples). Subsamples were: composited by biocrust location and treatment combinations within a plot, flash frozen in the field with liquid N, and stored at −20 • C until DNA analysis We extracted genomic DNA from 0.5 g of soil using the PowerSoil DNA Isolation Kit (MoBio, Carlsbad, CA) and amplified the V4-V5 region of the 16S rRNA gene using the bacterial specific primer set 515F and 806R with a 12-nt error correcting Golay barcodes (Aanderud et al, 2019). We used the following thermal cycle for PCR reactions: an initial denaturation step at 94 • C for 3 min followed by 35 cycles of denaturation at 94 • C for 45 s, annealing at 55 • C for 30 s, and an extension at 72 • C for 90 s. The amplified DNA was purified (Agencourt AMPure XP PCR Purification, Beckman Coulter Inc., Brea, CA, USA), pooled at approximately equimolar concentrations (Quant-iT PicoGreen dsDNA Kit, Invitrogen Corporation, Carlsbad, CA, USA), and sequenced at the Brigham Young University DNA Sequencing Center (http:// dnasc.byu.edu/) using a 454 Life Sciences Genome Sequence FLX (Roche, Branford, CT, USA).…”