Corrigendum: Scalable and Selective β‐Hydroxy‐α‐Amino Acid Synthesis Catalyzed by Promiscuous l‐Threonine Transaldolase ObiH

Abstract

Compound 22 was incorrectly drawn as the isomer being derived from 1-napthaldehyde, when it was instead derived from 2-napthaldehyde. Compound 15 should also be drawn as a carboxylate, and not a carboxylic acid. A corrected Figure 2 is provided. A correction to the corresponding text has also been made on Page 4, paragraph 2: "Instead, to further probe the limits of the ObiH active site, we sought to challenge the enzyme with sterically bulky aromatic aldehydes to generate napth-1-ylserine ( 22) and biphenylserine (23) (Figure 2)." Should state: "Instead, to further probe the limits of the ObiH active site, we sought to challenge the enzyme with sterically bulky aromatic aldehydes to generate napth-2-ylserine (22) and biphenylserine (23) (Figure 2).

Investigation of β‐Substitution Activity of O‐Acetylserine Sulfhydrolase from Citrullus vulgaris

“…All NMR chemical shifts were referenced either to a residual solvent peak or TMS internal standard. Spectra recorded using DMSO-d 6 were referenced to the residual DMSO signal at 2.5 ppm for 1 H and 39.52 ppm for 13 C NMR spectroscopy. Signal positions were recorded in ppm with the abbreviations s, d, t, q, dd, br, m, and app denoting singlet, doublet, triplet, quartet, doublet of doublets, broad, multiplet, and apparent respectively.…”

“…Enzymes operate under mild conditions with no protecting groups and can be combined in cascades to build complex molecules from simple precursors in a regio-and stereoselective fashion. [11][12][13] Identifying and characterizing new classes of enzymes that can meet the demands of preparativescale synthesis will provide additional tools for the facile synthesis of desirable nsAAs.…”

Engineered Biocatalytic Synthesis of β‐N‐Substituted‐α‐Amino Acids

“…[16,17] More recently, biocatalytic systems have been leveraged to synthesize ncAAs that avoid protecting group manipulations and a variety of CÀ C bond forming transformations have been developed (vida infra). [18][19][20][21][22][23][24][25][26][27] Despite these successes, methods that yield N-containing sidechains have been limited. The potential reversibility of CÀ N bond formation presents a distinct challenge and, if it were overcome, would enable access to new classes of densely functionalized ncAAs.…”