Corrigendum: Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study

Miguel-Pastor, Laura; Satué, Katy; Chicharro, Déborah; Torres-Torrillas, Marta; Romero, Ayla Del; Peláez, Pau; Carrillo, José M.; Cuervo, Belén; Sopena, Joaquín; Cerón, José J.; Rubio, Mónica

doi:10.3389/fvets.2022.954516

Cited by 1 publication

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Feline samples collected in sodium citrate tubes were immediately centrifugated at room temperature in a PRGF ® -Endoret ® System IV centrifuge (BTI Biotechnology Institute S.L.) under a single centrifugation protocol of 265 g for 10 min as described by Miguel-Pastor et al ( 33 ).…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of both GFs in both plasma fractions (PPP and PRP) were determined using an ELISA kit of development with antibodies to human (Human TGF-beta1 DuoSet ELISA de R&D Systems DY240-05 and Human PDGF-BB DuoSet ELISA de R&D Systems DY220, respectively), following the methodology previously published by Miguel-Pastor et al ( 33 ). To the knowledge of the authors, there are no commercial kits for GF determination in cats, so human kits were used for GFs determination as described by other researchers ( 27 , 51 ).…”

Section: Methodsmentioning

confidence: 99%

“…Although different protocols for obtaining PRP in cats have been described ( 27 – 32 ), the use of PRGF ® -Endoret ® technology in this species has only been reported in a previous study carried out by the same researchers. Due to the cellular characteristics of the autologous blood product obtained following centrifugation, it was classified as a P-PRP product ( 33 ). Despite this, there is no clinical evidence of its application, and therefore future research is needed in this field of feline internal medicine ( 28 , 29 , 33 ).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the cellular characteristics of the autologous blood product obtained following centrifugation, it was classified as a P-PRP product ( 33 ). Despite this, there is no clinical evidence of its application, and therefore future research is needed in this field of feline internal medicine ( 28 , 29 , 33 ).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the fact that GFs promote wound healing or tissue regeneration in musculoskeletal injuries, such as osteoarthritis in dogs ( 20 – 22 ), horses ( 23 ), rabbits ( 26 ), and humans ( 49 , 50 ), the authors considered that GFs could be an advantageous therapeutic option in cats with FeLV. For this reason, the objective of the study was to determine PLT concentrations and two specific types of GFs, such as Platelet-Derived Growth Factor-BB (PDGF-BB) and Transforming Growth Factor-ß1 (TGF-ß1), in both PPP and PRP fractions following the PRGF ® -Endoret ® protocol previously standardized for its application in cats by these same researchers ( 33 ). The authors hypothesize that a P-PRP can be obtained in FeLV cats following PRGF ® -Endoret ® methodology as in other species or in healthy cats.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of Platelet-Rich Plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-ß1 valuation

et al. 2023

Self Cite

View full text Add to dashboard Cite

IntroductionFeline leukemia virus (FeLV) is a chronic disease that leads to the weakening of a cat's immune system. Platelet-rich plasma (PRP) offers therapeutic effects for multiple diseases, the use of PRP and growth factors (GFs) determination could be an alternative treatment to improve the quality of life in these patients. The objectives of this study were to determine and compare the concentration of platelets (PLTs), red blood cells (RBCs) and white blood cells (WBCs) between samples of whole blood (WB), PRP and platelet-poor plasma (PPP) fractions, and to evaluate the concentration of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in both fractions in FeLV cats using a PRGF®-Endoret® protocol previously standardized in this species.MethodsWB was collected from 11 asymptomatic FeLV-positive cats. PRP and PPP was obtained following PRGF®-Endoret® technology according to centrifugation at 265 g for 10 min. Cellular components, RBCs, WBCs, PLTs, and the PDGF-BB and TGF-β1 concentrations in PRP and PPP fractions were determined.ResultsPLT in the PRP fraction was statistically higher than WB and PPP fraction, with no statistical differences between WB and PPP. PLT concentration increased 1.4 times in PRP fraction compared to WB. Mean platelet volume (MPV) did not differ significantly between the WB, PRP, and PPP fractions. Compared to WB, the absolute numbers of RBCs and WBCs were decreased by 99% and more than 95% in the PRP and PPP fractions, respectively. TGF-ß1 concentrations increased in PRP vs. PPP, with no changes in PDGF-BB.DiscussionBased on the degree of PLT enrichment and the absence of RBCs and WBCs, this blood product could be classified as a Pure Platelet-Rich Plasma (P-PRP). The presence of GFs in PRP and PPP samples suggests that the PRGF®-Endoret® methodology is suitable for obtaining PRP in FeLV cats, despite future studies are necessary to optimize the technique, standardize the results and assess clinical efficacy.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%